|Table of Contents|

Optimisation of Culture Media for Wild and Mutant Strains of Stropharia rugosoannulata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年6
Page:
104-110
Research Field:
Publishing date:

Info

Title:
Optimisation of Culture Media for Wild and Mutant Strains of Stropharia rugosoannulata
Author(s):
FU Luqin1; WANG Xinyue1; LUAN Qianqian2; YU Haili3; QIAN Lei1; BAN Litong1
(1.College of Agronomy and Resources Environment，Tianjin Agricultural University，Tianjin 300384;2.Gansu Academy of Agricultural Engineering Technology，Lanzhou，Gansu 730030;3.Wuwei Academy of Agricultural Sciences,Wuwei，Gansu 733000)
Keywords:
wild Stropharia rugosoannulata; mutant strain; antagonistic test; mother culture medium
PACS:
S 646
DOI:
10.11937/bfyy.20243772
Abstract:
The wild burgundy strain of Stropharia rugosoannulata and its white and yellow mutant strains were used as the test materials to screen the most suitable mother culture medium using one-way and orthogonal tests，in order to provide reference for the utilization of excellent strains of Stropharia rugosoannulata.The results showed that there was a significant antagonistic reaction among the three strains，and the one-way test indicated that the optimal carbon source for the burgundy strain and the white mutant was sucrose，the optimal nitrogen source was peptone，and the optimal inorganic salts were calcium chloride and potassium sulphate.The optimal carbon sources for the yellow mutant strain were soluble starch and sucrose，the optimal nitrogen sources were bran and yeast paste，the optimal inorganic salt was potassium sulphate，and the optimal vitamin was vitamin B.The yellow mutant strain had the fastest growing mycelium，which was vigorous，white and dense.Orthogonal tests determined that the optimal medium formulation for the yellow mutant strain was 200 g·L-1 potato，100 g·L-1 dried rice straw，15 g·L-1 sucrose，5 g·L-1 soluble starch，1 g·L-1 yeast paste，1.5 g·L-1 bran，1 g·L-1 potassium sulphate，and 10 mg·L-1 vitamin B，and the mycelial growth rate was 2.27 mm·d-1.There were some differences in mycelial growth of all three strains on different media，and the rice straw extract promoted mycelial growth，and the incubation time of the yellow mutant strain was shortened by 6 days compared with that of the PDA medium.

References:

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Last Update: 2025-04-03