|Table of Contents|

Isolation and Identification of Pathogenic Bacteria Causing Rot in Small Watermelon Fruits and Study on the Antibacterial Effect of ε-polylysine

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年7
Page:
99-106
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Pathogenic Bacteria Causing Rot in Small Watermelon Fruits and Study on the Antibacterial Effect of ε-polylysine
Author(s):
LI Ting1; 2; WANG Yunxiang2; 3; 4; WANG Baogang2; 3; 4; ZHOU Jiahua2; 3; 4; XU Ranran2; 3; 4; WANG Zhengrong1
(1.College of Life Science and Food Engineering，Hebei University of Engineering，Handan,Hebei 056107;2.Institute of Agricultural Products Processing and Food Nutrition，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097;3.Beijing Key Laboratory of Preservation and Processing of Fruits and Vegetables and Agricultural Products，Beijing 100097;4.Key Laboratory of Postnatal Treatment of Vegetables，Ministry of Agriculture and Rural Affairs，Beijing 100097)
Keywords:
small thin-skinned watermelon; spoilage bacteria; ε-polylysine; bacteriostasis
PACS:
S 651
DOI:
10.11937/bfyy.20243709
Abstract:
Taking Beijing thin skinned watermelon as the test material，the main pathogenic bacteria on the naturally occurring fruit of thin skinned watermelon during storage were studied by the methods of isolation and purification，morphological observation and fungal rDNA its sequence analysis;on this basis，the inhibitory effect of chitosan/ε-polylysine composite membrane on pathogenic bacteria was further discussed.Plate counting method，cross measurement method，drying constant weight method，microplate reader method and other detection methods were used to study the effects of different ε-polylysine additions (3%，5%，7%，9%) on the mortality rate of spoilage bacteria，mycelium growth inhibition rate，mycelium dry weight，spoilage bacteria conductivity，nucleic acid and protein extravasation degree，clarify the main pathogenic bacteria species of post harvest decay of thin skinned watermelon and the inhibitory effect of ε-polylysine on pathogenic bacteria，and in order to provide reference for the storage of thin skinned watermelons in the later stage.The results showed that the main pathogens causing watermelon decay during storage were identified as Botrytis cinerea，Aspergillus niger and Fusarium incarnatum.Combined treatment with chitosan/ε-polylysine could increase the mortality of putrefactive bacteria，delay the growth rate of mycelium，destroy the cell structure of Botrytis cinerea，Aspergillus niger and Fusarium incarnatun，increase the electrical conductivity value，increase the dissolution rate of protein，increase the exosmosis of nucleic acids，and finally lead to the death of watermelon putrefactive bacteria.

References:

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Last Update: 2025-04-22