|Table of Contents|

Genetic Diversity Analysis and Molecular ID Construction of Twenty-three Strains of Lyophyllum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年4
Page:
8-14
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis and Molecular ID Construction of Twenty-three Strains of Lyophyllum
Author(s):
LIU NaSONG YingZHANG MinLYU LitaoLI YueHUANG Lei
(Edible Fungi Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)
Keywords:
Lyophyllumgenetic diversitySSR molecular markersDNA molecular ID
PACS:
S 646
DOI:
10.11937/bfyy.20242986
Abstract:
Twenty-three Lyophyllum strains were used as test materials,the genetic diversity of germplasm resources and the construction of molecular identity were studied by using the analysis of mycelial growth morphology and SSR molecular marker technology,in order to provide reference for germplasm identification and efficient utilization of Lyophyllum.The results showed that the 23 strains showed significant differences in mycelial growth rate on PDA integrated medium,ranging from 1.138 mm·d-1 to 2.577 mm·d-1,and most of the mycelium showed radial pattern with weak margins.Polymorphic loci were abundant,ten pairs of SSR primers were amplified to obtain 39 polymorphic loci in 23 Lyophyllum strains and with the number of polymorphic loci ranging from 2 to 7 for each pair of primers.The genetic phase divergence coefficient of the test strains was between 0.14 and 0.82,and when the phase divergence coefficient was at 0.54,the strains could be divided into 6 groups.The QR code molecular ID of 23 Lyophyllum strains were constructed based on clear bands amplified from 10 pairs of SSR primers.

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Last Update: 2025-02-28