|Table of Contents|

Salt-tolerant Gene Mapping of Flax Using BSA-Seq Analysis

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年3
Page:
27-34
Research Field:
Publishing date:

Info

Title:
Salt-tolerant Gene Mapping of Flax Using BSA-Seq Analysis
Author(s):
ZHANG Yanping1; YE Chunlei1; QI Yanni2; CHEN Jun1; ZHAO Wei2; CHAI Zongwen3
(1.Institute of Biotechnology，Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070；2.Crop Research Institute，Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070；3.Gansu Provincial Agricultural Technology Extension Station，Lanzhou,Gansu 730020)
Keywords:
flax; BSA-Seq; salt resistance gene
PACS:
S 853.75
DOI:
10.11937/bfyy.20242807
Abstract:
The salt-tolerant STS and salt-sensitive DYMS and their hybrid offspring RIL populations of flax were used as the test materials,the BSA-Seq method was used，QTLs related to flax salt resistance were studied and screened，in order to provide reference for innovative cultivation of salt-tolerant germplasm and molecular marker-assisted breeding of flax.The results showed that 14 SNPS related to flax salt tolerance were selected after a series of analyses.8 SNP loci were screened on chromosome 2，2 SNP loci were screened on chromosome 3，and 1 SNP loci was screened on chromosome 5，6，9 and 13,respectively.Overexpression verified by real-time fluorescence quantitative PCR showed that the Lus_GLEAN_10024365 gene was a salt-tolerance candidate gene.It was on chromosome 2，which was scaffold208 on loci 5 913 with a length of 4 981 bp.It was found that Lus_GLEAN_10024365 was related to the synthesis of plasmodesmata callose (β-1，3-glucose polymer) binding protein which was deposited during salt stress.The correlation between this gene and the synthesis and metabolism of callose will be further investigated.

References:

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Last Update: 2025-02-13