|Table of Contents|

Effects of Potassium Salt Stress on Physiology and Corresponding Gene Expression in the Mycelia of Morchella sextelata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年3
Page:
18-26
Research Field:
Publishing date:

Info

Title:
Effects of Potassium Salt Stress on Physiology and Corresponding Gene Expression in the Mycelia of Morchella sextelata
Author(s):
LI Juan12SONG Jie12LIU Xiaohong12WANG Xu13LI Xiaohong12
(1.School of Agriculture,Liaodong University,Dandong,Liaoning 118003;2.Institute of Advanced Characteristic Agriculture Studies,Liaodong University,Dandong,Liaoning 118001;3.Institute of Yalu River Basin Studies,Liaodong University,Dandong,Liaoning 118001)
Keywords:
potassium salt stressMorchella sextelataantioxidant enzymesgene expression
PACS:
S 646.7
DOI:
10.11937/bfyy.20242646
Abstract:
The mycelium of Morchella sextelata were selected as the experimental material.Solid and liquid cultures were used,with KCl concentration at final concentrations of 0,0.1,0.2,0.3,0.4,0.5 mol·L-1,respectively.The effects of potassium salt stress on the mycelial growth characteristics,physiological indicators,and corresponding gene expression levels were studied,in order to provide reference for the study of salt tolerance physiology and optimization of cultivation conditions of Morchella spp..The results showed that with the increase of KCl concentration,the mycelial growth was inhibited,manifested as slender mycelium,increased branching,increased yellow pigment,inability to form fungal nuclei,and a decrease in growth rate.The inhibition rate of growth rate was 23.00% to 76.11%.With the increase of KCl stress concentration,the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) increased.The content of antioxidant active substances ascorbic acid (ASA) and glutathione (GSH) also showed an increasing trend.The activities of antioxidant enzymes SOD,CAT,GPX and GR showed a trend of first increasing and then decreasing,and reached the highest value under 0.3 mol·L-1 KCl concentration.The relative expression levels of genes MnSOD,CAT1,GPX,and GR showed a trend of first increasing and then decreasing,and reached the highest value under 0.3 mol·L-1 KCl concentration.Comprehensive analysis showed that potassium salt stress inhibited the mycelial growth and nucleation of M.sextelata,accelerates mycelial aging,and promoted the content of reactive oxygen.Under low concentration potassium salt stress,the mycelium reduced oxidative damage by regulating the expression of key genes involved in antioxidant enzymes and activating the antioxidant enzyme system.However,under high concentration potassium salt stress,the expression level of antioxidant enzyme genes decreases and enzyme activity was inhibited,and the mycelium mainly responds to oxidative damage by increasing the content of antioxidant substances ASA and GSH.

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Last Update: 2025-02-13