|Table of Contents|

Analysis of Salt-alkali Tolerance of Wild Type and Hair Root Gshdz4 Transformation in Trifolium pratense

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年5
Page:
74-82
Research Field:
Publishing date:

Info

Title:
Analysis of Salt-alkali Tolerance of Wild Type and Hair Root Gshdz4 Transformation in Trifolium pratense
Author(s):
TANG Jixiang1; ZHANG Junfeng2; LIU Mengyu1; LIU Yuhang2; LI Yanping2; CAO Lei1
(1.College of Horticulture and Landscape Architecture，Northeast Agricultural University，Harbin，Heilongjiang 150030；2.College of Geography and Tourism，Harbin University，Harbin，Heilongjiang 150030)
Keywords:
Leguminosae; Trifolium pratense; salt and alkali resistance; Gshdz4
PACS:
S 541+.2
DOI:
10.11937/bfyy.20242368
Abstract:
Taking seeds of Trifolium pratense as the test material，the methods of treating the seeds and seedlings of Trifolium pratense with 40，80，120，160，and 320 mmol·L-1 as well as NaHCO3 solutions at concentrations of 20，40，60，80,and 160 mmol·L-1 were used.Various growth parameters included germination potential and root length were examined under these stress conditions.Plant phenotypes were observed at different time points (0，4,8,24，32，44，48 hours and 52 hours),and the proportion of affected plants was determined,in order to provide insights into improving alkaline tolerance and breeding alkali-resistant chimera Trifolium pratense varieties.The results showed that the wild type could tolerate salt stress up to a concentration of 80 mmol·L-1and alkali stress up to a concentration of 40 mmol·L-1.The alkali-tolerant gene GSHDZ4-GFP was introduced into the root system of Trifolium pratense using Agrobacterium K599.The expression pattern of the Gshdz4 gene in Trifolium pratense roots was detected using laser confocal microscopy by observing green fluorescent protein signals.Super oxide dismutase (SOD) activity and malondialdehyde (MDA) contents were measured in both non-transgenic and transgenic roots.The results indicated that transplantation of Gshdz4 increased the tolerance of plant roots to alkali stress.

References:

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Last Update: 2025-03-14