|Table of Contents|

Study on Extraction and Purification of Flavonoids From Hippophae rhamnoides Leaves

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年23
Page:
88-96
Research Field:
Publishing date:

Info

Title:
Study on Extraction and Purification of Flavonoids From Hippophae rhamnoides Leaves
Author(s):
LIU Ruoqi; LIU Rui; MA Fulin; MA Yuhua
(College of Agriculture and Animal Husbandry，Qinghai University，Xining，Qinghai 810016)
Keywords:
leaf flavonoids; Hippophae rhamnoides; response surface methodology; extraction; purification
PACS:
S 793.6
DOI:
10.11937/bfyy.20242263
Abstract:
The mature leaves of Hippophae rhamnoides subsp.sinensis Rousi collected from Menyuan county，Qinghai Province，were used as the experimental material.A single-factor experiment and response surface methodology were employed to investigate and optimize the extraction and purification process of flavonoids from Hippophae rhamnoides leaves and to enhance the extraction efficiency and purification outcomes,in order to provide reference for the effective utilization of flavonoid compounds from Hippophae rhamnoides leaves.The results showed that under the conditions of 75% ethanol，an extraction temperature of 81 ℃，an extraction time of 110 minutes，and a material-to-liquid ratio of 1∶8 mg·mL-1，the theoretical yield of flavonoids was 60.56 mg·g-1，with an actual measured yield of (60.52±0.18)mg·g-1 after two ethanol extractions.Using D101 macroporous resin as the adsorbent，systematic optimization of parameters such as sample loading，concentration，pH environment，eluent volume，elution rate，and eluent concentration was conducted.The D101 macroporous resin exhibited an adsorption rate of 91.19%±0.32% and a desorption rate of 92.27%±0.75%，resulting in nearly a 2.95-fold increase in flavonoid content，with the adsorption rate surpassing previous reports.

References:

［1］陈学林，马瑞君，孙坤，等．中国沙棘属种质资源及其生境类型的研究［J］．西北植物学报，2003，23(3)：451-455．［2］WANG H，LIU H，YANG M，et al．Phylogeographic study of Chinese seabuckthorn (Hippophae rhamnoides subsp．sinensis Rousi) reveals two distinct haplotype groups and multiple microrefugia on the Qinghai-Tibet Plateau［J］．Ecology and Evolution，2014，4(22)：4370-4379．［3］MA X，LAAKSONEN O，ZHENG J，et al．Flavonol glycosides in berries of two major subspecies of sea buckthorn (Hippophae rhamnoides L．) and influence of growth sites［J］．Food Chemistry，2016，200：189-198．［4］CRISTE A，URCAN A C，BUNEA A，et al．Phytochemical composition and biological activity of berries and leaves from four Romanian Sea buckthorn (Hippophae rhamnoides L．) varieties［J］．Molecules，2020，25(5)：1170．［5］GUO R，GUO X，LI T，et al．Comparative assessment of phytochemical profiles，antioxidant and antiproliferative activities of Sea buckthorn (Hippophae rhamnoides L．) berries［J］．Food Chemistry，2017，221：997-1003．［6］侯霄．沙棘黄酮药理作用的研究进展［J］．国际沙棘研究与开发，2009，7(2)：5-7，11．［7］曹群华,瞿伟菁,黄晓青,等.沙棘籽渣和果渣中黄酮抗脂质过氧化、清除自由基作用［J］.中成药,2003(8):68-71.［8］于凤,许凯歌,许秋双,等.大孔吸附树脂分离纯化白子菜总黄酮工艺优化［J］.中成药,2023,45(9)：2827-2831.［9］魏增云,陈金娥,张海容.沙棘的活性化学成分与医疗应用［J］.忻州师范学院学报,2010,26(5):46-48.［10］田建华,张春媛,魏璐.沙棘果渣总黄酮提取工艺优化及抗氧化活性研究［J］.天然产物研究与开发,2021,33(1):65-72.［11］刘莹.从沙棘中提取黄酮类化合物的研究［J］.化学与生物工程,2005,22(7):50-51.［12］王树林.沙棘叶黄酮提取工艺研究［J］.食品研究与开发,2008(8):110-113.［13］王佳琪,李敏敏,范蓓,等.大孔树脂纯化滇黄精黄酮工艺优化及纯化前后抗氧化活性比较［J］.饲料研究,2024(7):74-81.［14］杜国军,洪婉婷,李占锋,等.大孔树脂吸附法纯化酸枣仁总黄酮的工艺研究［J］.中国调味品,2024,49(1):100-106.［15］曹雪丽,韩笑,李屿恺,等.响应面法优化泰山女儿茶总黄酮提取工艺及其抗氧化活性研究［J］.广东化工,2024,51(6):7-10.［16］高德艳,朱双全,王超萍,等.玫瑰花渣中黄酮的提取与纯化工艺研究［J］.中国果菜,2024,44(2):35-40.［17］蔡晓霞,李伯超,海来约布,等.唐古碎米荠总黄酮的富集纯化工艺及体外抗氧化活性研究［J］.中华中医药杂志,2022,37(10):5923-5828.［18］谭颂严,杨志伟.苦丁茶总黄酮纯化工艺及其黄嘌呤氧化酶抑制活性研究［J］.食品研究与开发,2022,43(9):124-131.［19］上官宇晨,黄丽媛,黄熊,等.大孔树脂纯化仙草黄酮及成分测定［J］.中国调味品,2021,46(6):149-152.［20］田洪霞,牛生洋.响应面法优化麦冬黄酮纯化工艺及其对运动疲劳的影响［J］.粮食与油脂,2023,36(12):84-88.［21］杨喜花,陈敏.沙棘叶中总黄酮的提取和纯化［J］.山西医科大学学报,2007(11):1005-1009.［22］李淑珍,武飞,陈月林,等.沙棘叶黄酮纯化工艺优化［J］.安徽师范大学学报(自然科学版),2015,38(6)：567-571.［23］张程慧,祁玉霞,冯叙桥.沙棘黄酮分离纯化方法的研究进展［J］.保鲜与加工,2019,19(4):201-206.［24］吴翠芳,刘雅宣,李宇辉,等.沙棘中黄酮类化合物提取及抗氧化活性［J］.安徽科技学院学报,2024,38(1):88-96.［25］苏慧慧,许菲,吴定坤,等.响应面优化三叶木通总黄酮的提取工艺及其抗氧化活性［J］.北方园艺,2022(21):100-105.［26］王军宪,王启祥,李星海,等.沙棘叶黄酮甙元类成分研究初报［J］.沙棘,1997(3):33-34.［27］谭诗涵,雷雅婷,张也,等.HPD100与D101大孔吸附树脂纯化禾本科芦笋总黄酮与总酚酸研究［J］.中国药师,2020,23(5):839-844.［28］高海立,郁吉锋,黄路瑶,等.铁皮石斛叶总黄酮的大孔树脂纯化工艺及抗氧化活性［J］.浙江理工大学学报(自然科学版),2019,41(3):380-386.［29］汤如莹,王玉杰,李伟,等.D101型大孔吸附树脂分离纯化沙苑子总黄酮工艺研究［J］.中草药,2017,48(16):3342-3346.［30］丁伟杰,胡盈,陈娈,等.D101型大孔吸附树脂分离纯化红蓼总黄酮工艺研究［J］.中国医院药学杂志，2014,34(15):1259-1262.［31］黄莎,刘燕梅,昌琴,等.D-101大孔吸附树脂纯化沙棘叶黄酮的特性研究［J］.食品安全导刊,2022,357(28):156-159.［32］王玉,刘琦,耿杰.沙棘黄酮的分离纯化及其抗运动性疲劳作用［J］.食品工业科技,2020,41(23):169-174.

Memo

Memo:
-
Last Update: 2024-12-16