|Table of Contents|

Analysis of Lignin Content and Synthesis Gene CAD Expression in Head-splitting-prone and Head-splitting-tolerant Cabbage

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年24
Page:
1-7
Research Field:
Publishing date:

Info

Title:
Analysis of Lignin Content and Synthesis Gene CAD Expression in Head-splitting-prone and Head-splitting-tolerant Cabbage
Author(s):
DING Xiaoya1; 2; YUE Zhichen2; ZHAO Yanting2; LI Biyuan2; ZANG Yunxiang1; TAO Peng2
(1.College of Horticulture Science，Zhejiang A&F University，Hangzhou，Zhejiang 311300；2.Institute of Vegetables，Zhejiang Academy of Agricultural Sciences，Hangzhou，Zhejiang 310021)
Keywords:
head cabbage; head-splitting; lignin; CAD; gene expression
PACS:
S 635.1
DOI:
10.11937/bfyy.20242255
Abstract:
Taking head-splitting-prone and head-splitting-tolerant cabbage as the test materials,the relationship between the head-splitting phenotype and lignin content was studied by using a combination of phloroglucinol staining and transcriptomics,in order to uncover the potential role of lignin in the process of cabbage head splitting.The results showed that the degree of lignification in the petioles of head-splitting-prone cabbage and the lignin content in the leaves were slightly higher than those of head-splitting-tolerant cabbage.Cinnamyl Alcohol Dehydrogenases (CAD)，the key genes for lignin biosynthesis，were identified across the entire genome of head cabbage.A total of 10 CAD genes were obtained and distributed on 6 chromosomes of head cabbage；there were significant differences in exon-intron structure between CAD genes in head cabbage；the expression of most CAD genes were detected in leaves of both types of head cabbage.Five CAD genes ([STBX]BoCAD4，BoCAD7-LF，BoCAD7-MF2a，BoCAD7-MF2b，and BoCAD9-MF2[STBZ]) showed higher expression in head-splitting-prone cabbage than in head-splitting-tolerant cabbage，which corresponding to lignin assay results.The results of this study showed that there was no significant correlation between lignin content and head-splitting phenotype in head cabbage.Lignin was not the main factor affecting head-splitting phenotype in head cabbage.

References:

［1］杨丽梅,方智远,张扬勇,等.“十三五” 我国甘蓝遗传育种研究进展［J］.中国蔬菜,2021(1):15-21.［2］苏彦宾.结球甘蓝耐裂球性状遗传效应分析及QTL定位［D］.北京：中国农业科学院，2012.［3］苏彦宾.结球甘蓝高密度遗传图谱构建及耐裂球、球色、球形基因定位［D］.北京:中国农业大学,2015.［4］尹婧,范伟强,张红,等.卫青萝卜糠心发生与相关成分变化规律研究［J］.北方园艺,2022(16):97-106.［5］黄汉唐.木质素代谢相关基因在柚果实发育过程中的作用机理初探［D］.福州:福建农林大学,2020.［6］GALL D L，RALPH J,DONOHUE T J,et al.Biochemical transformation of lignin for deriving valued commodities from lignocellulose［J］.Current Opinion in Biotechnology,2017,45:120-126.［7］耿飒,徐存拴,李玉昌.木质素的生物合成及其调控研究进展［J］.西北植物学报,2003,23(1):171-181.［8］胡文冉,郝晓燕,赵准,等.棉花CAD基因家族的生物信息学分析［J］.生物技术进展,2023,13(3):412-424.［9］JANNOEY P,CHANNEI D,KOTCHARERK J,et al.Expression analysis of genes related to rice resistance against brown planthopper,Nilaparvata lugens［J］.Rice Science,2017,24(3):163-172.［10］SIBOUT R,EUDES A,MOUILLE G,et al.CINNAMYL ALCOHOL DEHYDROGENASE-C and-D are the primary genes involved in lignin biosynthesis in the floral stem of Arabidopsis［J］.The Plant Cell,2005,17(7):2059-2076.［11］KIM S，KIM K，CHO M，et al.Expression of cinnamyl alcohol dehydrogenases and their putative homologues during Arabidopsis thaliana growth and development:Lessons for database annotations［J］.Phytochemistry，2007，68(14)：1957-1974.［12］LIU W,JIANG Y,WANG C,et al.Lignin synthesized by 〖STBX〗CmCAD2 〖STBZ〗and 〖STBX〗CmCAD3 〖STBZ〗in oriental melon (Cucumis melo L.) seedlings contributes to drought tolerance［J］.Plant Molecular Biology,2020,103(6):689-704.［13］王倩,余坤江,王显亚,等.木质纤维素合成与调控机制:从模式植物到油菜［J］.中国油料作物学报,2024,46(2):303-311.［14］ZHAO Q,DIXON R A.Altering the cell wall and its impact on plant disease:From forage to bioenergy［J］.Annual Review of Phytopathology,2014,52:69-91.［15］TAKAYUKI H，KAZUYUKI W.Role of the plant cell wall in gravity resistance［J］.Phytochemistry,2015，112：84-90.［16］杨为海,曾辉,邹明宏,等.裂果发生与果皮细胞壁修饰的关系研究进展［J］.热带作物学报,2011,32(10):1995-1999.［17］WANG Y,LI Q,ZHANG G,et al.Mechanism of tolerance to head-splitting of cabbage (Brassica oleracea L.var.capitata L.):A review of current knowledge and future directions［J］.Horticulturae,2023,9(2):251.［18］曾爱松,刘玉梅,方智远,等.甘蓝耐裂球性与叶表面微形态及细胞组织结构的关系研究［J］.华北农学报,2009,24(S2):41-45.［19］王丽娟,秦智伟.春甘蓝裂球性的解剖特征［C］.杭州:中国园艺学会首届青年学术讨论会,1994.［20］WANG J,GAO X,MA Z,et al.Analysis of the molecular basis of fruit cracking susceptibility in Litchi chinensis cv.Baitangying by transcriptome and quantitative proteome profiling［J］.Journal of Plant Physiology,2019,234:106-116.［21］LIU Y L,CHEN S Y,LIU G T,et al.Morphological,physiochemical,and transcriptome analysis and 〖STBX〗CaEXP4 〖STBZ〗identification during pepper (Capsicum annuum L.) fruit cracking［J］.Scientia Horticulturae,2022,297:110982.［22］张钰佳,崔凯文,段力升,等.海岛棉CAD和CAD-Like基因家族的鉴定、表达及其对大丽轮枝菌的响应［J］.中国农业科学,2023,56(19):3759-3771.［23］DONG N Q,LIN H X.Contribution of phenylpropanoid metabolism to plant development and plant-environment interactions［J］.Journal of Integrative Plant Biology,2021,63(1):180-209.［24］隋娟娟,邓红祥,杨京霞,等.香椿〖STBX〗TsCAD1〖STBZ〗基因的克隆与非生物胁迫下的表达特性［J］.基因组学与应用生物学,2019,38(10):4617-4625.

Memo

Memo:
-
Last Update: 2025-01-07