|Table of Contents|

Genetic Diversity Analysis of Pugionium Gaertn. Resources Based on SSR and SRAP Markers

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年2
Page:
1-7
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of Pugionium Gaertn. Resources Based on SSR and SRAP Markers
Author(s):
MEN Yan1; YANG Zhongren1; HUANG Xiumei1; ZHANG Xiaohui2; ZHANG Fenglan1; HAO Lizhen1
(1.College of Horticulture and Plant Protection，Inner Mongolia Agricultural University，Hohhot，Inner Mongolia 010000；2.Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Beijing 100081)
Keywords:
Pugionium Gaertn.; genetic diversity; phylogenetic; SSR marker; SRAP marker
PACS:
S 949.748.3
DOI:
10.11937/bfyy.20242066
Abstract:
Taking the leaves of Pugionium Gaertn. plants and parts of brassicaceae plants as experimental materials,the genetic diversity and phylogenetic relationships of Pugionium Gaertn. were studied by using SSR and SRAP molecular marker techniques，in order to provide reference for the application Pugionium Gaertn..The results showed that the average number of effective alleles,the average percentage of polymorphic loci,the average values of Nei′s and Shannon′s detected by 7 pairs of primers were 1.260 7,54.88%,0.159 3 and 0.264 4，respectively；75.18% of genetic differentiation occurs within Pugionium cornutum (L.) Gaertn.and within various types of Pugionium dolabratum Maxim，while 24.82% occurs between species and within various types of Pugionium dolabratum Maxim.The SRAP amplification results showed that the average number of effective alleles,the average percentage of polymorphic loci,the average values of Nei′s and Shannon′s were 1.703 2，70.32%，0.234 2 and 0.353 3，respectively；80.18% of the genetic differentiation occurs within the types and 19.82% was between types.SSR analysis showed that the genetic diversity level of the Pugionium cornutum (L.) Gaertn.was lower than that of the Pugionium dolabratum Maxim.The genetic diversity level of the special types was the highest，followed by the broadwing type，calcanthus type，crown type and axe type.SRAP analysis of the phylogenetic relationship between the Pugionium Gaertn.plants and parts of the Brassicaceae family showed that the Pugionium Gaertn.had a relatively distant phylogenetic relationship with the tested Brassica,Raphanus,Clerodendrum,Orychophragmus and Arabidopsis.

References:

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Last Update: 2025-02-08