|Table of Contents|

Study on Tissue Culture Rapid Propagation and Tissue Micro-cuttage Technology of Pyrus betulifolia

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年20
Page:
23-29
Research Field:
Publishing date:

Info

Title:
Study on Tissue Culture Rapid Propagation and Tissue Micro-cuttage Technology of Pyrus betulifolia
Author(s):
GUAN QiuzhuDONG XiaochangDONG RanJIAO HuijunWANG HongweiWEI Shuwei
(Shandong Fresh Pear Cultivation and Breeding Engineering Technology Research Center,Shandong Institute of Pomology,Tai′an,Shandong 271000)
Keywords:
Pyrus betulifolia Bungetissue cultureshoot proliferationrootingmicro-cutting
PACS:
S 661.2
DOI:
10.11937/bfyy.20241744
Abstract:
Tissue culture plantlets of 4 Pyrus betulifolia lines originated from seed were used as the test materials.The methods of plant tissue culture and tissue culture seedling micro-cutting were adopted.The effects of different concentrations of 6-BA singly or in combination with IBA on the in vitro shoot proliferation and the influence of different concentrations of IBA or NAA on rooting of micro-shoots by in vitro and ex vitro was studied,in order to provide reference for the establishment of the tissue culture and rapid proliferation system of Pyrus betulifolia.The results showed that the genotype and 6-BA concentration had a highly significant effect on the proliferation coefficient,and the IBA concentration had a significant effect on the proliferation coefficient.As for root induction,low concentration of auxin was better than high concentration.DL30 showed higher proliferation coefficient and rooting rate.The optimum medium for DL30 shoot proliferation was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 IBA,with a proliferation coefficient 3.4±0.35.For the in vitro rooting experiments,the highest rooting rate of DL30 was 60.0%±20.0% on the medium containing 1/2MS+0.2 mg·L-1 IBA.For the ex vitro rooting experiments,rooting of micro-shoots was significantly influenced by auxin applied as quick-dip method,and the highest rooting rate (46.7%±8.8%) was obtained in DL30 at the treatment of 1 000 mg·L-1 IBA.

References:

[1]杨军,曹玉芬,吴俊,等.杜梨实生繁殖群体遗传多样性的SSR分析[J].西北植物学报,2011,31(11):2172-2177.[2]赵明新,曹继礼,曹刚,等.梨新栽培模式发展中存在问题及对策分析[J].北方园艺,2023(17):146-152..[3]WERTHEIM S J.Rootstocks for European pear:A review[J].Acta Horticulturae,2002(596):299-309.[4]冀明辉,李龙飞,高丽娟,等.梨砧木研究进展[J].河北农业科学,2022,26(1):76-80,97.[5]闫帅,徐锴,袁继存,等.梨组织培养及遗传转化研究进展[J].中国果树,2017(S1):72-77.[6]THAKUR A,DALAL R,Navjot.Micropropagation of pear (Pyrus spp.):A review[J].Agricultural Reviews,2008(29):260-270.[7]RUIC〖JX-+0.8mm〗〖KG-*2〗',VUJOVIC〖JX-+0.8mm〗〖KG-*2〗' T,CEROVIC〖JX-+0.8mm〗〖KG-*2〗' R.In vitro multiplication of semi-dwarfing pear rootstock ‘Pyrodwarf’ in relation to cytokinin types[J].Acta Horticulturae,2016,1139:279-284.[8]孙清荣,关秋竹,孙洪雁.西洋梨品种‘红考密斯’的组织培养及离体叶片不定梢再生[J].山东农业科学,2018,50(11):28-32.[9]方明,欧春青,王斐,等.4种梨试管苗组织培养和生根体系的建立[J].中国南方果树,2019,48(5):74-78.[10]刘敏,苏乔,刘纪文.‘欧美杨107’组培苗瓶外生根[J].植物生理学通讯,2010,46(10):1050-1054.[11]叶伟,刘凉琴,史学正,等.基质中添加多孔改良剂对南方高丛蓝浆果品种‘蓝美1号’组培苗茎段瓶外生根的影响[J].植物资源与环境学报,2020,29(3):69-71.[12]李昌珠,SEDLAK J,JAN B.不同基因型欧洲梨离体繁殖研究[J].果树学报,2002,19(4):227-230.[13]IGLESIAS I,VILARDELL P,BONANY J,et al.Micropropagation and field evaluation of the pear (Pyrus communis L.) ‘IGE 2002’,a new selection of the cultivar dr.jules guyot[J].Journal of the American Society for Horticultural Science,2004,129(3):389-393.[14]AYGUN A,DUMANOGLU H.In vitro shoot proliferation and in vitro and ex vitro root formation of Pyrus elaeagrifolia Pallas[J].Frontiers in Plant Science,2015(6):225.[15]苗冉冉,乔月莲,吴沅洙,等.‘津香蜜’和‘巴梨’组织培养快速繁殖[J].分子植物育种,2019,17(7):2297-2302.[16]孙清荣,孙洪雁,刘庆忠.丰水梨的组培快繁研究[J].落叶果树,2001,33(4):4-5.[17]叶宇,欧春青,王斐,等.梨矮化砧木中矮4号组培快繁技术研究[J].中国果树,2023(9):11-15.[18]杨冠宇,王斐,张艳杰,等.杜梨砧木组培扩繁技术研究[J].中国果树,2022(6):59-63.[19]罗嘉亮,李凡,李俊豪,等.继代培养次数对杜梨生根能力和叶形态及其激素水平的影响[J].西北植物学报,2020,40(2):304-310.[20]赵亚楠,张懿,谭旭,等.杜梨组培苗两步生根技术体系优化[J].中国农业大学学报,2021,26(11):105-112.[21]卢明艳,闫兴凯,王强,等.抗寒耐盐碱梨砧木优系 “1-127” 增殖与生根技术研究[J].中国南方果树,2020,49(6):129-132.[22]RODRIGUEZ R,DAZ-SALA C,CUOZZO L,et al.Pear in vitro propagation using a double-phase culture system[J].HortScience,1991,26(1):62-64.

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Last Update: 2024-10-29