|Table of Contents|

Effects of Heat Stress on Physiological Characteristics of Lentinus edodes Mycelium

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年22
Page:
95-100
Research Field:
Publishing date:

Info

Title:
Effects of Heat Stress on Physiological Characteristics of Lentinus edodes Mycelium
Author(s):
ZHANG Jun; JIANG Yu; SUN Shuijuan; DU Shipu
(Sanmenxia Agricultureal Science Research Institute,Sanmenxia,Henan 472000)
Keywords:
Lentinus edodes; heat stress; physiological characteristics; comprehensive evaluation
PACS:
S 646.1+2
DOI:
10.11937/bfyy.20241478
Abstract:
The fungus bags of ‘Yuxiang No.1’‘L18’‘Nanshan No.1’ were used as the test materials.Different high temperature stress intensities (30 ℃-12 h，33 ℃-24 h，and 36 ℃-36 h) were used to heat stress the fungus bags.The effects of heat stress on the physiological characteristics of the mycelium of the three Lentinus edodes varieties were studied by measuring the recovery growth rate of Lentinus edodes mycelium，malondialdehyde (MDA) content，hydrogen peroxide (H2O2) content，proline (Pro) content，anthranilate synthase (TrpE)，and laccase activity.The principal component analysis and factor analysis methods were used to comprehensively evaluate the heat resistance，in order to clarify the relationship between each index and the heat stress of Lentinus edodes mycelium，and provide reference for further screening of heat-resistant germplasm of Lentinus edodes.The results showed that with the increasing of stress intensity，the recovery growth rate of mycelium in three mushroom varieties significantly decreased,compared with the control,the malondialdehyde content increased. The malondialdehyde content of ‘Nanshan No.1’ and ‘Yuxiang No.1’ in C treatment was lower than that in B treatment.The hydrogen peroxide content of ‘L18’ in C treatment was lower than that in the control.The hydrogen peroxide content of the three mushroom varieties in other treatments was higher than that in the control.The proline content of ‘Nanshan No.1’ and ‘Yuxiang No.1’ was significantly higher than the control，showing a continuous upward trend，while the proline content of ‘L18’ was lower than the control.As the intensity of the treatment stress increased,the TrpE activity of ‘Nanshan No.1’ was gradually decreasing trend，‘L18’ was first increasing and then decreasing，and ‘Yuxiang No.1’ was showing a gradually increasing trend.The activity of laccase showed a trend of first increasing and then decreasing.Comprehensive evaluation using principal component analysis and factor analysis showed that the degree of stress was low,the heat resistance was in the order of ‘L18’>‘Yuxiang No.1’>‘Nanshan No.1’.As the stress intensifies，its heat resistance was ‘Nanshan No.1’>‘Yuxiang No.1’>‘L18’.

References:

［1］常婷婷,赵妍,杨焕玲,等.食药用菌高温胁迫应答研究进展［J］.食用菌学报,2021,28(1):124-134.［2］刘秀明,黄晨阳,陈强,等.外源海藻糖对高温胁迫下肺形侧耳氧化损伤的缓解效应［J］.园艺学报,2013,40(8):1501-1508.［3］ALLAN A C,FLUHR R.Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells［J］.The Plant Cell,1997,9(9):1559-1572.［4］WAHID A,GELANI S,ASHRAF M,et al.Heat tolerance in plants:An overview［J］.Environmental and Experimental Botany,2007,61(3):199-223.［5］PINHERO R G,RAO M V,PALIYATH G,et al.Changes in activities of antioxidant enzymes and their relationship to genetic and paclobutrazol-induced chilling tolerance of maize seedlings［J］.Plant Physiology,1997,114(2):695-704.［6］徐超.苗期高温对草莓生长发育和果实品质的影响机理及模拟研究［D］.南京:南京信息工程大学,2021.［7］夏建红,汪宏亮,郭菊梅,等.北方地区香菇棚栽越夏出菇技术［J］.北方园艺,2022(13):152-156.［8］韩蕊莲,李丽霞,梁宗锁.干旱胁迫下沙棘叶片细胞膜透性与渗透调节物质研究［J］.西北植物学报,2003,23(1):23-27.［9］刘君娣.杏树三品种抗旱性研究［D］.兰州:甘肃农业大学,2007.［10］林金科.水分胁迫对茶树光合作用的影响［J］.福建农业大学学报,1998,27(4):423-427.［11］BOUSLAMA M,SCHAPAUGH W T.Stress tolerance in soybeans.I.evaluation of three screening techniques for heat and drought tolerance［J］.Crop Science,1984,24(5):933-937.［12］魏良明,贾了然,胡学安,等.玉米抗旱性生理生化研究进展［J］.干旱地区农业研究,1997,15(4):66-71.［13］陆艾鲜,凌瑞,陈生煜,等.高温胁迫下8个绣球品种的生理生化响应［J］.热带作物学报,2022,43(4):816-828.［14］马超君.香菇耐高温相关邻氨基苯甲酸合酶基因功能分析［D］.武汉:华中农业大学,2018.［15］赵晶,李旭彤,梁学忠,等.陆地棉漆酶基因家族鉴定及在黄萎病菌胁迫下的表达分析［J］.作物学报,2019,45(12):1784-1795.［16］YE J,ZHONG T,ZHANG D,et al.The auxin-regulated protein ZmAuxRP1 coordinates the balance between root growth and stalk rot disease resistance in maize［J］.Molecular Plant,2019,12(3):360-373.［17］章毅,韦孟琪,孙欧文,等.不同绣球品种对干旱胁迫的生理响应及抗旱机制研究［J］.西北林学院学报,2018,33(1):90-97.

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Last Update: 2024-12-03