|Table of Contents|

Mating-type Molecular Identification of Phlebopus portentosus Homokaryotic Strains

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年22
Page:
101-108
Research Field:
Publishing date:

Info

Title:
Mating-type Molecular Identification of Phlebopus portentosus Homokaryotic Strains
Author(s):
CAO Yang1; 2; 3; LUO Shunzhen1; HE Chunmei1; JI Guangyan1; SHU Ping3; JI Kaiping1; 2
(1.Jinghong Hongzhen Agricultural Science and Technology Co.Ltd.，Jinghong，Yunnan 666100；2.Guizhou Hongzhen Fungus Industry Investment and Development Co.Ltd.，Zhenfeng，Guizhou 562200；3.Dali Comprehensive Inspection Centre of Quality and Technical Supervision，Dali，Yunnan 671000)
Keywords:
Phlebopus portentosus; mating-type; identification; SNP marker
PACS:
S 646.3
DOI:
10.11937/bfyy.20241213
Abstract:
Heterokaryotic parent strains and their offspring homokaryotic strains of Phlebopus portentosus were used as the test materials.β-flanking gene and ste3.2 gene，which respectively represent mating-type A and B genotype，was used to develop molecular markers to study the mating-type identification of P.portentosus homokaryotic strains,in order to provide reference for improving the work efficiency of its cross breeding.The results showed that 5 pairs the primers could amplify 91% of the β-flanking gene region，other 5 pairs could amplify 71% of the ste3.2 gene region.Amplification sequencing was successfully performed on 2 heterokaryotic parent strains using all primers above although the usable sequence lengths and SNP sites numbers were visibly different.Based on the results of first round PCR，primers beta2 and 3.22 were chosen for the second round PCR and mating-typing of HZ19110 homokaryotic strains.There were 5，4，4 and 4 homokaryotic strains of mating-type A1B1,A2B2，A1B2，and A2B1 respectively.Primers beta5 and 3.25 were chosen for the second round PCR and mating-typing of HZ20029 homokaryotic strains.There were 4，3，3 and 3 homokaryotic strains of mating-type A3B3,A4B4，A3B4，and A4B3 respectively.The mating-type identification of homokaryotic strains was consistent with the results of pairwise culture and clamp screening under microscope.Mating-type of P.portentosus homokaryotic strains from different heterokaryotic parent strains could be rapidly and accurately identified by using the molecular methods in this study.The breeding efficiency of this species could be consequently improved.

References:

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Last Update: 2024-12-03