|Table of Contents|

Effects of Melatonin Soaking on Seed Germination and Physiological Indexes of Cowpea Under Low Temperature Stress

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年20
Page:
8-15
Research Field:
Publishing date:

Info

Title:
Effects of Melatonin Soaking on Seed Germination and Physiological Indexes of Cowpea Under Low Temperature Stress
Author(s):
ZENG ChuntaoZHANG ShuyueQIU XuewenWANG NanLIANG JunjieWU Caijun
(College of Agriculture,Jiangxi Agricultural University,Nanchang,Jiangxi 330045)
Keywords:
cowpeaseedmelatonincold stressphysiological characteristics
PACS:
S 643.4
DOI:
10.11937/bfyy.20241006
Abstract:
Taking the seeds of the cowpea variety ‘Huagan Lyuxiu’ as the test materials,under the conditions of 0,50,100 and 200 μmol·L-1 melatonin (MT) concentration,undergoing a low temperature environment of 10 ℃,the germination rate,antioxidant enzyme activity,superoxide anion (O2) production rate,content of osmotic adjustment substances,and MDA content of cowpea seeds were studied under the treatment of melatonin,in order to provide reference for the application of cowpea in facility production.The results showed that,1) germination of cowpea seeds was inhibited under low-temperature stress,whereas the application of exogenous melatonin improved seed germination under low-temperature stress,whereas the germination rate was still lower than that of the control.2) Both peroxidase (POD) and catalase (CAT) activities in cowpea seeds reached a maximum on 4th day of the 200 μmol·L-1MT treatment,superoxide dismutase (SOD) activity peaked on 6th day of the 200 μmol·L-1MT treatment,whereas the MDA content peaked on 6th day of the 100 μmol·L-1MT treatment,and the rate of superoxide anion (O2) production reached a minimum on 6th day of the 50 μmol·L-1 MT treatment.3) The free proline content reached a maximum on 6th day of the 50 μmol·L-1MT treatment,the soluble protein content reached a maximum on 4th day of the 100 μmol·L-1MT treatment,and the soluble sugar content reached a maximum on 2nd day of the 200 μmol·L-1MT treatment.It was found that the application of exogenous melatonin under low-temperature stress conditions could increase the germination rate of seeds,enhance the activity of antioxidant enzymes,reduce the content of MDA,slow down the production rate of superoxide anion (O2),and promote the accumulation of osmotic-regulating substances,which could effectively alleviate the damage caused by low-temperature stress to cowpea seeds during germination,enhance their tolerance to low temperatures,and restore their normal growth ability.The effect was better when a concentration of 200 μmol·L-1was applied.

References:

[1]ZHANG H,ZHU J,GONG Z,et al.Abiotic stress responses in plants[J].Nature Reviews.Genetics,2022,23(2):104-119.[2]CAO Y,FENG J,HWARARI D,et al.Alterations in population distribution of Liriodendron chinense (hemsl.) sarg.and Liriodendron tulipifera linn.caused by climate change[J].Forests,2022,13(3):488.[3]于奇,曹亮,金喜军,等.低温胁迫下褪黑素对大豆种子萌发的影响[J].大豆科学,2019,38(1):56-62.[4]ZHANG B,TIEMAN D M,JIAO C,et al.Chilling-induced tomato flavor loss is associated with altered volatile synthesis and transient changes in DNA methylation[J].Proceedings of the National Academy of Sciences of the United States of America,2016,113(44):12580-12585.[5]TAN D X,MANCHESTER L C,TERRON M P,et al.One molecule,many derivatives:A never-ending interaction of melatonin with reactive oxygen and nitrogen species?〖KG-*4〗[J].Journal of Pineal Research,2007,42(1):28-42.[6]吕家龙.蔬菜栽培学各论[M].北京:中国农业出版社,1980.[7]王明友,张红,李士平.低温胁迫对不同豇豆品种抗寒性生理特性的影响[J].安徽农学通报,2016,22(24):60-62.[8]郭经纬,商桑,穆大伟,等.外源NO对低温胁迫下豇豆幼苗生长和生理特性的影响[J].热带作物学报,2015,36(12):2179-2183.[9]张献英,唐力生,犹昌艳,等.低温对豇豆种子萌发和出苗的影响[J].南方农业学报,2013,44(11):1785-1790.[10]赵兆.不同长豇豆品种(系)耐冷性综合评价及NO和GSH对其缓解效应的研究[D].雅安:四川农业大学,2022.[11]李颖,鱼小军,赵一珊,等.水杨酸和脱落酸浸种对低温下扁蓿豆种子萌发和幼苗生长的影响[J].草地学报,2021,29(1):174-181.[12]项洪涛,李琬,何宁,等.苗期低温胁迫下烯效唑对红小豆根系抗寒生理及产量的影响[J].草业学报,2019,28(7):92-102.[13]SHAHANI A A A,ABBAS A,HAMEED R,et al.Melatonin in plants:A pleiotropic molecule for abiotic stresses and pathogens infection[J].Scientia Horticulturae,2023,322:112387.[14]ARNAO M B,HERNNDEZ-RUIZ J.Melatonin in flowering,fruit set and fruit ripening[J].Plant Reproduction,2020,33(2):77-87.[15]HERNNDEZ-RUIZ J,ARNAO M.Relationship of melatonin and salicylic acid in biotic/abiotic plant stress responses[J].Agronomy,2018,8(4):33.[16]TAN X L,FAN Z Q,KUANG J F,et al.Melatonin delays leaf senescence of Chinese flowering cabbage by suppressing ABFs-mediated abscisic acid biosynthesis and chlorophyll degradation[J].Journal of Pineal Research,2019,67(1):e12570.[17]HOSSEINI M S,SAMSAMPOUR D,ZAHEDI S M,et al.Melatonin alleviates drought impact on growth and essential oil yield of lemon verbena by enhancing antioxidant responses,mineral balance,and abscisic acid content[J].Physiologia Plantarum,2021,172(2):1363-1375.[18]SINGH A,BANERJEE A,ROYCHOUDHURY A.Fluoride tolerance in rice is negatively regulated by the ‘stress-phytohormone’ abscisic acid (ABA),but promoted by ABA-antagonist growth regulators,melatonin,and gibberellic acid[J].Protoplasma,2022,259(5):1331-1350.[19]赵海亮,左璐,张璐,等.低温胁迫下外源褪黑素对番茄幼苗光抑制的缓解效应[J].应用生态学报,2023,34(1):151-159.[20]WU P,MA Y,AHAMMED G J,et al.Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumber[J].Frontiers in Plant Science,2022(13):1029854.[21]CAO Q,LI G,CUI Z,et al.Seed priming with melatonin improves the seed germination of waxy maize under chilling stress via promoting the antioxidant system and starch metabolism[J].Scientific Reports,2019(9):15044.[22]董倩.N-乙酰-L-半胱氨酸、褪黑素引发对低温胁迫下杂交水稻种子萌发和幼苗生长及基因表达的影响[D].杭州:浙江大学,2020.[23]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.[24] 高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.[25]张荣萍,马均,蔡光泽,等.开花期低温胁迫对四川攀西稻区水稻开花结实的影响[J].作物学报,2012,38(9):1734-1742.[26] 蔡子文,王宏霞,蔡子平,等.引发处理对不同温度下菘蓝(Isatis indigotica Fort.)种子萌发与幼苗生长的生态响应[J].北方园艺,2023(20):102-109.[27]SARKAR R K,BHOWMIK M,BISWAS SARKAR M,et al.Comprehensive characterization and molecular insights into the salt tolerance of a Cu,Zn-superoxide dismutase from an Indian Mangrove,Avicennia marina[J].Scientific Reports,2022(12):1745.[28]JI C Y,JIN R,XU Z,et al.Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato[J].BMC Plant Biology,2017,17(1):139.[29]罗太敏,向梅,须文.维生素B6浸种对低温胁迫下辣椒种子萌发和幼苗生理特性的影响[J].北方园艺,2023(18):9-17.[30]杨晓旭,李梦娣,刘大军,等.外源褪黑素对低温胁迫下菜豆种子萌发及抗性的影响[J].中国农学通报,2022,38(33):34-38.[31]DAT J,VANDENABEELE S,VRANOV E,et al.Dual action of the active oxygen species during plant stress responses[J].Cellular and Molecular Life Sciences:CMLS,2000,57(5):779-795.[32]王楚侨,黄一梅,罗弦,等.外源ABA对低温胁迫下火龙果苗活性氧代谢的影响[J].西北植物学报,2023,43(8):1344-1351.[33]SATO Y,MASUTA Y,SAITO K,et al.Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene,OsAPXa[J].Plant Cell Reports,2011,30(3):399-406.[34]SAMANTA S,BANERJEE A,ROYCHOUDHURY A.Melatonin application differentially modulates the enzymes associated with antioxidative machinery and ascorbate-glutathione cycle during arsenate exposure in indica rice varieties[J].Plant Biology,2021,23(Suppl 1):193-201.[35]DARVIZHEH H,ZAHEDI M,ABBASZADEH B,et al.Changes in some antioxidant enzymes and physiological indices of purple coneflower (Echinacea purpurea L.) in response to water deficit and foliar application of salicylic acid and spermine under field condition[J].Scientia Horticulturae,2019,247:390-399.[36]李贺,姜欣悦,陈忠诚,等.外源褪黑素对低温胁迫下大豆V1期幼苗光合荧光及抗氧化系统的影响[J].中国油料作物学报,2020,42(4):640-648.[37]HMMAM I,ALI A E M,SALEH S M,et al.The role of salicylic acid in mitigating the adverse effects of chilling stress on “seddik” mango transplants[J].Agronomy,2022,12(6):1369.[38]陈素玉.外源褪黑素对盐胁迫下大豆幼苗生长的影响[D].大庆:黑龙江八一农垦大学,2023.[39]ZHAO Q,CHEN S,WANG G,et al.Exogenous melatonin enhances soybean (Glycine max (L.) Merr.) seedling tolerance to saline-alkali stress by regulating antioxidant response and DNA damage repair[J].Physiologia Plantarum,2022,174(4):e13731.[40]LI Z G,GONG M.Mechanical stimulation-induced chilling tolerance in tobacco suspension cultured cells and its relation to proline[J].Russian Journal of Plant Physiology,2013,60(1):149-154.[41]LI Z G,YUAN L X,WANG Q L,et al.Combined action of antioxidant defense system and osmolytes in chilling shock-induced chilling tolerance in Jatropha curcas seedlings[J].Acta Physiologiae Plantarum,2013,35(7):2127-2136.[42]江登辉,刘晓颖,陈乾,等.干旱胁迫对福建山樱花和日本樱花叶片渗透调节物质的影响[J].福建农林大学学报(自然科学版),2020,49(6):772-778.[43]蒋健.外源褪黑素对低温胁迫下花椰菜种子萌发及幼苗生长的影响[D].泰安:山东农业大学,2023.[44]和秋兰,张航,王正维,等.外源褪黑素对低温胁迫下马铃薯幼苗抗氧化系统的影响[J].华北农学报,2022,37(1):103-111.[45]蒋朝维,吴俊杰,姜浩,等.低温胁迫下烤烟幼苗对喷施外源褪黑素的生理响应[J].江苏农业科学,2023,51(21):62-66.

Memo

Memo:
-
Last Update: 2024-10-29