|Table of Contents|

Screening of Culture Substrates and Exploration of Living Condition of Hohenbuehelia serotina

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年23
Page:
105-113
Research Field:
Publishing date:

Info

Title:
Screening of Culture Substrates and Exploration of Living Condition of Hohenbuehelia serotina
Author(s):
WANG Xin1; 2; JING Ruiyong1; 2; XIA Yuqiao1; 2; WU Nan1; LI Ximei1; 2; WANG Liyan1; 2
(1.College of Life Science and Biotechnology，Heilongjiang Bayi Agricultural University/Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Region，Daqing，Heilongjiang 163319；2.Key Laboratory of Low-Carbon Green Agriculture in Northeast Plain，Ministry of Agriculture and Rural Affairs，Daqing，Heilongjiang 163319)
Keywords:
Hohenbuehelia serotina; straw partial substitution for wood chips; degradation rate of lignocellulose; extracellular enzyme activity; living condition
PACS:
S 646
DOI:
10.11937/bfyy.20240986
Abstract:
Taking Hohenbuehelia serotina as the test material，culture substrates of Hohenbuehelia serotina were selected by replacing partly (40%) wood chips with 5 kinds of crop straws，the single factor test method was used，effects of different culture conditions on the mycelial growth rate，extracellular enzyme activity，degradation rate of lignin，cellulose and hemicellulose，substrate weight loss rate of Hohenbuehelia serotina were studied,in order to provide reference for cultivating Hohenbuehelia serotina with crop straw partial substitution for wood chips.The results showed that among the five kinds of crop straws，Hohenbuehelia serotina mycelial growth rate were no significant difference between soybean straw，corn straw and all wood chips treatment.Soybean and corn straw can replace part of wood chips according to weight loss rate，degradation rate of cellulose，hemicellulose and lignin，and activity of 5 kinds of extracellular enzymes.In the experiment of exploring the living conditions of Hohenbuehelia serotina with single factor，the ratio of carbon to nitrogen was 27∶1，pH 7.0，the ratio of material to water was 1∶1.8 g·mL-1 and the temperature of 22-25 ℃ by analyzing all indexes measured.Correlation analysis showed that there was a strong coupling between mycelial growth rate and extracellular enzyme activity，lignocellulose degradation rate and weight loss rate.In conclusion，optimal crop straw partial substitution for wood chips were corn and soybean straw，the optimum living conditions of Hohenbuehelia serotina are carbon-nitrogen ratio of 27∶1，pH value of 7.0，material-water ratio of 1∶1.8 g·mL-1 and temperature of 22-25 ℃.

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Last Update: 2024-12-16