|Table of Contents|

Screening，Isolation and Identification of Naematelia aurantialba Strains

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年18
Page:
108-113
Research Field:
Publishing date:

Info

Title:
Screening，Isolation and Identification of Naematelia aurantialba Strains
Author(s):
TIAN Shuang; ZHU Jianan; MA Qingfang; ZHANG Piqi; DAI Xiaodong; LIU Jianing
(Institute of Microbiology，Heilongjiang Academy of Sciences，Harbin，Heilongjiang 150010)
Keywords:
Naematelia aurantialba; mycelium growth; cultivation; isolation and identification
PACS:
S 646.6
DOI:
10.11937/bfyy.20240825
Abstract:
Taking 7 Naematelia aurantialba strains as the test materials，the mycelium growth of different strains in cPDA solid plate medium was compared，and the growth of different strains was studied through the cultivation test，and the production strains were separated and molecular identification,in order to provide reference for the selection of excellent strains during the cultivation of Naematelia aurantialba.The results showed that,according to the growth of the 7 production strains，it could be seen that the mycelium growth rate of ‘Naematelia aurantialba 1’‘Naematelia aurantialba 2’‘Naematelia aurantialba 5’ and ‘Naematelia aurantialba 7’ had higher mycelium growth rate.The cultivation test results showed that all the 7 strains of Naematelia aurantialba could produce ear，but ‘Naematelia aurantialba 1’ had the best comprehensive ear growth effect.It was preliminarily confirmed that ‘Naematelia aurantialba 1’ could be used as a production strain.Two fungi were isolated from ‘Naematelia aurantialba 1’ by spore separation and intrabasal mycelium separation.The two fungi were identified as Naematelia aurantialba and Stereum complicatum by ITS sequencing and evolutionary relationship analysis.

References:

［1］杨林雷,李荣春,曹瑶,等.金耳的学名及分类地位考证［J］.食药用菌,2020,28(4):252-255,276.［2］YUAN Q,ZHANG X,MA M,et al.Immunoenhancing glucuronoxylomannan from Tremella aurantialba Bandoni et Zang and its low-molecular-weight fractions by radical depolymerization:Properties,structures and effects on macrophages［J］.Carbohydrate Polymers,2020,238:116184.［3］DENG C,SUN Y,FU H,et al.Antioxidant and immunostimulatory activities of polysaccharides extracted from Tremella aurantialba mycelia［J］.Molecular Medicine Reports,2016,14(5):4857-4864.［4］FAN J,CHU Z,LI L,et al.Physicochemical properties and microbial quality of Tremella aurantialba packed in antimicrobial composite films［J］.Molecules,2017,22(3):500.［5］曹瑶,李荣春,杨林雷,等.工厂化栽培金耳的营养成分测定及品质评价［J］.食药用菌,2021,29(4):318-322.［6］佚名.我国多地发展金耳栽培［J］.食药用菌,2021,29(4):291.［7］李东梅,张思维,孙建云,等.不同产地金耳中氨基酸含量分析及营养价值评价［J］.食品安全质量检测学报,2024,15(1):237-244.［8］叶彬.金耳菌种的分子鉴定及出菇实验［D］.福州:福建农林大学,2012.［9］张俊波,刘绍雄,华蓉,等.金耳新品种‘中菌金耳4号’选育［J］.食用菌学报,2023,30(6):33-44.［10］岳万松,李雪松,刘绍雄,等.金耳栽培常见问题及对策探讨［J］.中国食用菌,2022,41(8):80-85.［11］杨学英.金耳液体制种及袋料栽培技术研究［D］.汉中:陕西理工大学,2021.［12］钟冬季,钟秀媚.金耳栽培技术［J］.食用菌,2008,30(4):50-51.［13］刘书畅,李荣春,马布平,等.金耳菌种生产技术研究［J］.中国食用菌,2019,38(9):94-99.［14］武倩.金耳有效菌种鉴定及制备技术研究［D］.保定:河北农业大学,2020.［15］徐玉妹,张润清.基于因子分析联合熵值法的河北省食用菌种植综合效益评价［J］.北方园艺,2022(3):138-144.［16］李建英,罗孝坤,刘春丽,等.金耳有效原种的配方和品种筛选［J］.生物灾害科学,2020,43(1):96-99.

Memo

Memo:
-
Last Update: 2024-10-11