|Table of Contents|

Identification of Pathogen Causing Anthracnose of Pepper (Capsicum annuum L.) in Gansu Province

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年15
Page:
1-7
Research Field:
Publishing date:

Info

Title:
Identification of Pathogen Causing Anthracnose of Pepper (Capsicum annuum L.) in Gansu Province
Author(s):
JING Zhuoqiong1; 2; GUO Zhijie1; 2; SUN Qian1; 2; XU Shengjun1; 2
(1.Institute of Plant Protection，Gansu Academy of Agricultural Sciences，Lanzhou，Gansu 730070；2.Scientific Observing and Experimental Station of Crop Pests in Tianshui，Ministry of Agriculture and Rural Affairs，Tianshui，Gansu 741200)
Keywords:
anthracnose of pepper; isolation and identification; multilocus phylogenetic analysis; Colletotrichum fioriniae
PACS:
S 436.418
DOI:
10.11937/bfyy.20240808
Abstract:
Taking diseased chili fruits with typical anthracnose symptoms collected from Anning district，Lanzhou city，Gansu Province as the test materials，taking tissue isolation method to isolate pathogens，a multi gene combined phylogenetic tree，and combining morphology and Koch′s rule were constructed to identify the fungal species causing pepper anthracnose in Lanzhou city,in order to provide reference for the diagnosis and prevention and control of this disease.The results showed that 13 strains with the same culture characteristics were obtained from typical anthracnose pepper fruits.The assay of Koch′s postulates confirmed that CA1902 was the causal agent of pepper anthracnose that were identified as Colletotrichum fioriniae by morphology and multilocus phylogenies from combined datasets of ITS，TUB2,ACT,CHS-1,HIS3 and GAPDH.The isolate CA1902 clustered with C.fioriniae，having 100% bootstrap support.According to the above,the pathogen causing anthracnose of pepper (Capsicum annuum L.) was Colletotrichum fioriniae in Anning district,in Lanzhou,Gansu Province.This study was the first report of C.fioriniae causing anthracnose on pepper in Gansu Province，China.Considering the economic value of pepper，this identification maight aid in the selection of appropriate management tactics for this disease.

References:

［1］邹学校,朱凡.辣椒的起源、进化与栽培历史［J］.园艺学报,2022,49(6):1371-1381.［2］王培先,李正南,张磊,等.辣椒种质抗炭疽病水平鉴定及 炭疽病菌药剂敏感性分析［J］.北方园艺,2022(21):1-8.［3］LAKSHMESHA K K,LAKSHMIDEVI N,MALLIKARJUNA S A.Changes in pectinase and cellulase activity of Colletotrichum capsicimutants and their effect on anthracnose disease on capsicum fruit［J］.Archives of Phytopathology and Plant Protection,2005,38(4):267-279.［4］DIAO Y Z,ZHANG C,LIU F,et al.Colletotrichum species causing anthracnose disease of chili in China［J］.Persoonia,2017,38:20-37.［5］XU X,ZHANG Z,WANG Y,et al.First report of Colletotrichum fioriniae causing anthracnose disease of Rhus chinensis in China［J］.Plant Disease,2023,107(12):4034.［6］CHANG J,ZHAI F,ZHANG Y,et al.Identification and characterization of Colletotrichum fioriniae and C.fructicola that cause anthracnose in pecan［J］.Frontiers in Plant Science,2022(13):1043750.［7］HU S,PENG L,DING H,et al.First report of Colletotrichum fioriniae causing anthracnose on Rhododendron delavayi in China［J］.Plant Disease,2022,106(11):2995.［8］XU S J,JING Z Q,GUO Z J,et al.First report of anthracnose disease caused by Colletotrichum fioriniae on barbary wolfberry (Lycium barbarum L.) in Gansu Province,China［J］.Plant Disease,2022,106(9):2523.［9］LAI J L,BAI C H,PAN J M,et al.Colletotrichum horii and Colletotrichum fioriniae causing anthracnose on star anise (Illicium verum) in China［J］.Plant Disease,2023,107(10):3294.［10］WANG Q H,KUANG S Y,ZENG Y C,et al.First report of Colletotrichum fioriniae causing leaf spot on Schima superba in the world［J］.Plant Disease,2022,106(9):2524.［11］LIU H,LI D,ZHANG T,et al.First report of anthracnose on Cinnamomum camphora (camphor tree) caused by Colletotrichum fioriniae and Colletotrichum siamense in China［J］.Plant Disease,2022,106(5):1519.［12］但雨柔,刘铭,崔馨燕,等.多花黄精炭疽病病原菌鉴定［J］.植物保护,2023,49(2):288-293.［13］DAMM U,CANNON P F,WOUDENBERG J H C,et al.The Colletotrichum acutatum species complex［J］.Studies in Mycology,2012,73:37-113.［14］张翠文,魏立娟,杨成德.甘肃省辣椒炭疽病菌的分离鉴定及生物学特性［J］.西北农业学报,2023,32(10):1627-1636.［15］SREENIVASAPRASAD S,TALHINHAS P.Genotypic and phenotypic diversity in Colletotrichum acutatum,a cosmopolitan pathogen causing anthracnose on a wide range of hosts［J］.Molecular Plant Pathology,2005,6(4):361-378.［16］SHIVAS R G,TAN Y P.A taxonomic re-assessment of Colletotrichum acutatum，introducing C.fioriniae comb.et stat.nov.and C.simmondsii sp.nov［J］.Fungal Diversity,2009,39:111-122.［17］MACKENZIE S J,PERES N A,BARQUERO M P,et al.Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida［J］.Phytopathology,2009,99(5):620-631.［18］POLASHOCK J J,OUDEMANS P V,CARUSO F L,et al.Population structure of the North American cranberry fruit rot complex［J］.Plant Pathology,2009,58:1116-1127.［19］IVIC D,VONCINA D,SEVER Z,et al.Identification of Colletotrichum species causing bitter rot of apple and pear in Croatia［J］.Journal of Phytopathology,2013,161(4):284-286.［20］MUNDA A.First report of Colletotrichum fioriniae and C.godetiae causing apple bitter rot in Slovenia［J］.Plant Disease,2014,98(9):1282.［21］NODET P,BARONCELLI R,FAUGRE D,et al.First report of apple bitter rot caused by Colletotrichum fioriniae in Brittany,France［J］.Plant Disease,2016,100(7):1497.［22］TASHIRO N,URAKAWA A,SHOJI K,et al.Postharvest anthracnose on Satsuma mandarin orange caused by Colletotrichum fioriniae［J］.Journal of General Plant Pathology,2018,84(5):381-385.［23］LING J F,PENG A T,JIANG Z D,et al.First report of anthracnose fruit rot caused by Colletotrichum fioriniae on litchi in China［J］.Plant Disease,2021,105(4):1225.［24］NIGAR Q,CADLE-DAVIDSON L,GADOURY D M,et al.First report of Colletotrichum fioriniae causing grapevine anthracnose in New York［J］.Plant Disease,2022,1077(1):223.［25］崔治家,邵晶,马毅,等.甘肃省道地药材枸杞子资源现状及产业发展对策研究［J］.中国现代中药,2023,9(1):15-21.［26］马琪,祁伟伟,杨盈,等.天水市苹果产业发展现状及对策［J］.北方果树,2023(3):50-51,59.

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Last Update: 2024-08-23