|Table of Contents|

Isolation and Identification of Pathogens of Actinidia arguta Rot and Antifungal Study of Garlic Crude Extract

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年17
Page:
34-42
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Pathogens of Actinidia arguta Rot and Antifungal Study of Garlic Crude Extract
Author(s):
QIAO Xinyue12DU Chunmei12LI Li3LI Lili12N.V.Skrypchenko24LIU Dejiang12
(1.School of Biology and Agriculture,Jiamusi University,Jiamusi,Heilongjiang 154007;2.China-Ukraine Agriculture & Forestry Technology Development and Application International Cooperation Joint Laboratory,Jiamusi,Heilongjiang 154007;3.Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan,Hubei 430074;4.M.M.Gryshko National Botanical Gardens of National Academy of Sciences of Ukraine,Kyiv,Ukraine 01014)
Keywords:
Actinidia argutarot pathogenisolation and identificationTalaromyces sp.garlic crude extract
PACS:
S 663.4
DOI:
10.11937/bfyy.20240660
Abstract:
Taking diseased Actinidia arguta fruit as the experimental materials,tissue isolation method,morphological and molecular biology identification methods were used to isolate and identify pathogens.The mycelium growth rate method was used to determine the inhibitory effect of garlic crude extract on pathogens,the fruit rot pathogens in Actinidia arguta in Jiamusi and the effects of garlic extract on these pathogens were investigated,in order to provide reference for scientific prevention and control of diseases.The results showed that seven kinds of pathogenic bacteria were isolated from the infected Actinidia arguta fruit,which were identified as Fusarium proliferatum,Penicillium sp.,Talaromyces sp.,Aspergillus niger,Pestalotiopsis sp.,Botrytis sp.and Neopestalotiopsis sp..Among which Neopestalotiopsis sp.had the strongest pathogenicity,and the decay of Actinidia arguta fruit caused by Talaromyces sp.was reported for the first time in China.The crude extract of garlic showed good antibacterial activity against 12 pathogenic fungi of Actinidia arguta fruit valsa canker,and the higher the concentration,the more obvious the inhibitory effect.When the concentration of garlic crude extract was 30 mg·mL-1,the inhibition rate of 12 pathogenic bacteria reached more than 60%,and the inhibition rate of Talaromyces sp.was up to 100%.

References:

[1]朴一龙,赵兰花.软枣猕猴桃研究进展[J].北方园艺,2008(3):76-78.[2]侯芳玉,陈飞,陆意,等.长白山产软枣猕猴桃茎多糖抗感染和抗肿瘤作用的研究[J].白求恩医科大学学报,1995,21(5):472-475.[3]刘延吉,刘金凤,田晓艳,等.软枣猕猴桃多糖降血糖降血脂活性研究[J].食品与生物技术学报,2012,31(1):86-89.[4]牛强,申健,刘悦,等.软枣猕猴桃主要活性成分及药理活性研究进展[J].食品工业科技,2019,40(3):333-338,344.[5]石浩,王仁才,王芳芳,等.猕猴桃软腐病主要病原菌的分离、鉴定及其生长特性研究[J].核农学报,2020,34(11):2425-2434.[6]李青芝,蒋亚涛,张莉娟,等.河南周口水果市场上猕猴桃软腐病主要病原真菌鉴定及防治研究[J].中国果树,2020(2):63-68.[7]尉莹莹,梁晨,赵洪海.软枣猕猴桃镰孢菌根腐病的病原[J].菌物学报,2017,36(10):1369-1375.[8]张路路.软枣猕猴桃内生真菌与腐败菌研究[D].佳木斯:佳木斯大学,2020.[9]熊萍萍.大蒜提取物的抗菌活性研究[D].南昌:南昌大学,2019.[10]程龙军,郭得平.葱蒜类作物中的蒜氨酸酶[J].植物生理学通讯,2001,37(5):471-474.[11]祁高展,李琼毅,李贞子,等.大蒜素对苹果青霉病的抑菌作用和防治效果[J].食品科技,2019,44(10):49-52.[12]张志敏,侯彦双,张兵,等.大蒜提取液对新鲜树莓保鲜效果的影响[J].食品工业科技,2021,42(9):299-305.[13]曾东方,陈玢,曾麟,等.大蒜素对三种农作物病原真菌的抑制作用[J].湖北农业科学,2011,50(11):2247-2249.[14]廖静静,刘屹湘,杨敏,等.大蒜挥发物和浸提液对辣椒疫霉菌的抑菌活性分析[J].云南农业大学学报(自然科学版),2014(3):337-346.[15]汤丽梅,周清,吴尚,等.大蒜素对猕猴桃果实致病菌的抑制活性及贮藏防效研究[J].中国南方果树,2014,43(4):15-18,33.[16]何敏.48种精油单组分的抗真菌作用规律及其对猕猴桃采后病害的控制效果[D].武汉:华中农业大学,2022.[17]苏文文,吴迪,韩振诚,等.贵州猕猴桃果实腐烂病病原菌鉴定及分析[J].河南农业科学,2021,50(3):97-102.[18]何海燕,付跃,秦文芳,等.原始森林高产纤维素酶刺器腐霉的筛选鉴定[J].食品研究与开发,2019,40(19):205-209.[19]刘丽珍,甄莉娜,崔乃忠,等.大蒜素的提取及抑菌效果研究[J].中国调味品,2021,46(2):79-82.[20]李梦玮,陈晓霞,补欢欢,等.花椒根腐病拮抗真菌的分离鉴定及其生防潜力探究[J].中国生物防治学报,2023,39(1):176-183.[21]孙剑秋,阮永明,金世宇,等.篮状菌属的重要性及其分类学研究概况[J].菌物研究,2021,19(2):83-93.[22]吴玉珠,陈娜,胡军华,等.青脆李枯萎病致病菌的分离鉴定及药剂筛选[J].湖南农业大学学报(自然科学版),2022,48(3):298-304.[23]WANG C W,AI J,QIN H Y,et al.First report of Sclerotinia nivalis causing Sclerotinia rot on hardy kiwifruit (Actinidia arguta) in China[J].Plant Disease,2016,100(9):1952.[24]KIM G H,CHOI D H,PARK S Y,et al.First report of anthracnose caused by Colletotrichum nymphaeae on kiwiberry in Korea[J].Plant Disease,2018,102(7):1455.[25]JIANG J W,LIANG P Y,CHENG S,et al.First report of damping-off in Actinidia argute (Tara Vine) caused by Fusarium proliferatum in China[J].Plant Disease,2020,104(2):578.[26]LIU J,GUO X,ZHANG H,et al.First report of postharvest fruit rot disease of hardy kiwifruit caused by Diaporthe eres in China[J].Plant Disease,2021,105(11):3745.[27]LI K,ZHANG C,WANG W,et al.First report of Neopestalotiopsis clavispora causing postharvest fruit rot on Actinidia arguta in Liaoning Province,China[J].Plant Disease,2023,107(1):217.[28]程小梅,彭亚军,杨玉,等.猕猴桃青霉病病原菌鉴定及中草药提取物对其抑菌效果[J].植物保护,2018,44(3):186-189,202.[29]李佩琪,江山,戴群,等.猕猴桃和青菜病原真菌的分离、鉴定及生物学特性[J].食品工业科技,2022,43(19):146-153.[30]裴艳刚,朱宇航,岁立云,等.四川猕猴桃灰霉病菌对4种杀菌剂的抗药性检测[J].植物保护,2021,47(4):180-185.[31]左盼盼,付苏,彭丽桃,等.猕猴桃采后软腐病病原菌鉴定及香芹酚对其控制效果[J].华中农业大学学报,2020,39(6):15-22.[32]韩盛,玉山江·麦麦提,潘俨,等.鲜杏采后病原菌鉴定及室内药剂筛选研究[J].新疆农业科学,2016,53(5):866-876.[33]赵淑艳,李喜宏,陈丽,等.桃采后病原菌种类及侵染规律研究[J].食品科学,2005,26(10):253-255.[34]李宁,关文强,段双科.葡萄采后致腐病原菌鉴定及侵染规律[J].保鲜与加工,2005,5(3):37-39.[35]MUKHTAR I,QUAN X,CHOU T,et al.First report of Talaromyces funiculosus causing fruit core rot of peach (Prunus persica) in China[J].Plant Disease,2019,103(8):2124.[36]马婷燕,李彦忠.32个紫花苜蓿品种的种带真菌种类及致病性研究[J].草业学报,2020,29(12):131-139.[37]马稳霞.黄花蒿精油、1,8-Cineole及Terpinen-4-ol对兰州百合采后病害防治研究[D].兰州:西北师范大学,2021.[38]苟亚峰,薛超,高圣风,等.斑兰叶叶部病害病原菌的分离鉴定[J].热带作物学报,2022,43(12):2527-2533.[39]张岱源,叶长文,李栋,等.湖北雪茄烟叶致霉菌分离鉴定及芽胞杆菌属拮抗菌株筛选[J].烟草科技,2023,56(6):8-16.[40]代惠芹,刘树泽,高琪,等.大蒜素处理对双孢菇保鲜效果的影响[J].北方园艺,2022(11):82-88.

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Last Update: 2024-09-24