|Table of Contents|

Establishment and Optimization of Tissue Culture System of Four Hosta plantagina Varieties

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年18
Page:
49-58
Research Field:
Publishing date:

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Title:
Establishment and Optimization of Tissue Culture System of Four Hosta plantagina Varieties
Author(s):
ZHU Chunyao1; SUN Xiaogang1; SUN Hongtong1; SHI Junmeng2; SONG Jiawang1
(1.College of Forestry and Grass Science,Jilin Agricultural University,Changchun,Jilin 130000;2.Jilin Hongyuan Landscape Design Engineering Co.Ltd.,Changchun,Jilin 130000)
Keywords:
Hosta plantagina; tissue culture; plant growth regulators; proliferation and differentiation; seedling transplanting
PACS:
S 682
DOI:
10.11937/bfyy.20240563
Abstract:
Taking the young shoot explants of four Hosta plantagina varieties as the test materials,the effects of different sterilisation times,phytohormone ratios and concentrations on the induction,proliferation and rooting of adventitious shoots,as well as the effects of different substrate formulations on seedling transplantation were investigated,in order to provide reference for enriching the resources of Hosta plantagina in the Northeast China,and for enriching the market demand as well as the factory production of Hosta plantagina.The results showed that the optimal sterilization method for explants was 75% alcohol 30 seconds+0.1% mercuric chloride solution for 8 minutes.The optimal formulations of clumping bud induction medium were H.‘Indigo Leaf’ MS+6-BA 3.5 mg·L-1+NAA 0.1 mg·L-1,H.‘Golden Tiara’ MS+6-BA 2.5 mg·L-1+NAA 0.2 mg·L-1,H.‘Flos Lotus’ and H.‘Blue Cadet’ MS+6-BA 2.5 mg·L-1+NAA 0.1 mg·L-1; H.‘Flos Lotus’and H.‘Blue Cadet’ showed high value-added of 4.06 and 6.71 times,respectively,under the induction of MS+6-BA 3.0 mg·L-1+NAA 0.1 mg·L-1,‘Golden Tiara’ showed 4.23 times under the induction of MS+6-BA 4.0 mg·L-1+NAA 0.1 mg·L-1.H.‘Indigo Leaf’ could proliferate 4.25-fold under the induction of MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1,and the optimal rooting medium was H.‘Indigo Leaf’ 1/2MS+IBA 0.5 mg·L-1,H.‘Golden Tiara’ and H.‘Blue Cadet’ 1/2MS+NAA 0.05 mg·L-1,and H.‘Flos Lotus’,1/2MS+NAA 0.1 mg·L-1.In the seedling refining test,the most suitable substrate for transplanting H.‘Flos Lotus’ and H.‘Blue Cadet’ tissue culture seedlings was the substrate of upper sand and lower soil (thickness 1∶1),and the survival rate was 100%.The H.‘Indigo Leaf’ and H.‘Golden Tiara’ were transplanted with sand∶soil (1∶1) mixed substrate for transplanting.

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Last Update: 2024-10-11