|Table of Contents|

Microbial Diversity Analysis of Trichosanthes kirilowii Maxim. Fruits and Identification of Pathogenic Pathomycete

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年18
Page:
100-107
Research Field:
Publishing date:

Info

Title:
Microbial Diversity Analysis of Trichosanthes kirilowii Maxim. Fruits and Identification of Pathogenic Pathomycete
Author(s):
XING Lingkai1CAO Shun2LI Weiwen3CHENG Youyu4ZHAO Wei2XIE Dongmei1
(1.College of Pharmacy,Anhui University of Chinese Medicine,Hefei,Anhui 230012;2.Institute of Plant Protection and Agro-products Safety,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230031;3.Institute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230031;4.Anhui You Yu Kua Yue Gua Lou Food Development Co.Ltd.,Qianshan,Anhui 246300)
Keywords:
Trichosanthes kirilowii Maxim.pathomycetenext generation sequencingmolecular identification
PACS:
S 567.9
DOI:
10.11937/bfyy.20240552
Abstract:
Taking healthy and diseased fruits of Trichosanthes kirilowii as the test material,next generation sequencing (NGS) technology and molecular identification method were used to analyze the healthy and diseased fruits to explore the species of pathomycete of T.kirilowii fruits,in order to provide reference for scientific prevention and control of diseases in Trichosanthes kirilowii.The results showed that a total of 8 phyla,24 orders,54 classes,133 families,206 genera,and 286 species were obtained from all samples.Among them,the top five genera were Diaporthe,Cladosporium,Stagonosporopsis,Colletotrichum and Hannaella.There was a high concentration of microorganisms on the diseased fruits,and the microbial community structure was significantly different from the healthy group.The microorganisms with high abundance were mainly Diaporthe and Colletotrichum.The fungus of Trichosanthes kirilowii Maxim. were isolated and purified.Four typical strains were selected for DNA molecular identification.The results showed that the fungi were Colletotrichum,Diaporthe,which verified the results of microbial diversity analysis.In summary,Diaporthe and Colletotrichum fungi were identified as the pathomycete causing the disease of T.kirilowii in Qianshan city.

References:

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Last Update: 2024-10-11