|Table of Contents|

Molecular Marker Detection of Resistance Genes in 105 Tomato Germplasm Resources

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年9
Page:
24-32
Research Field:
Publishing date:

Info

Title:
Molecular Marker Detection of Resistance Genes in 105 Tomato Germplasm Resources
Author(s):
FAN HuidongXU ShilinZHENG ShijinTIAN SongWANG YuweiGONG Qingrui
(Jilin Academy of Vegetable and Flower Sciences,Changchun,Jilin 130000)
Keywords:
tomatoresistance genefunctional markerbreeding for disease resistance
PACS:
S 641.2
DOI:
10.11937/bfyy.20233930
Abstract:
Taking 105 tomato germplasm resources as test materials and the resistance gene molecular marker assisted screening method was used to investigate the distribution of 8 resistance genes,included Mi-1,I-2,Cf-9,Ph-3,Ty-1,Ty-3,Tm-2a and Sw,in 105 germplasm resources,in order to provide reference for the development of resistance gene polymerization breeding.The results showed that 28 materials contained Mi-1,60 materials containing I-2,80 materials containing Cf-9,34 materials containing Ph-3,19 materials containing Ty-1,11 materials containing Ty-3,47 materials containing Tm-2a,and 7 materials containing Sw.Among 105 tomato materials,3 tomato materials did not contain any resistance gene.There were 78 materials containing less than 3 resistance genes,accounting for 74.3% of the total materials.There were 9 materials containing 4 resistance genes.There were 11 materials containing 5 resistance genes.There were 6 materials containing 6 resistance genes,namely XIHJ-21-27,DT-27-43,722-ZT77,722-ZT79,494,495.One of the materials containing 7 resistance genes was XLJ-226-41.No tomato material containing 8 resistance genes at the same time was detected.

References:

[1]李景富,崔亚男,姜景彬,等.抗旱番茄种质资源筛选及抗旱性评价[J].东北农业大学学报,2018,49(4):1-9.[2]董伟,张立平.蔬菜病虫害诊断与防治彩色图谱[M].北京:中国农业科学技术出版社,2012.[3]曲文姬.浅谈番茄抗病育种研究[J].农业开发与装备,2017(8):59.[4]张超越,韦建明,李云洲.番茄褪绿病毒研究进展[J].山地农业生物学报,2023,42(2):48-51.[5]向伟勇.番茄褐色皱纹果病毒对中国番茄产业的潜在威胁及预防措施[J].湖北农业科学,2021,60(S2):247-251.[6]冯建成.分子标记辅助选择技术在水稻育种上的应用[J].中国农学通报,2006,22(3):43-47.[7]魏偲,史倩倩,茆振川,等.Mi基因家族番茄对南方根结线虫的抗性鉴定及评价[J].中国蔬菜,2016(6):29-33.[8]胡明瑜,白文钦,潘晓雪,等.番茄抗晚疫病基因Ph-3的分子标记开发及应用[J].植物病理学报,2018,48(4):560-566.[9]郑于莉,刘燕,韩兰兰,等.抗番茄黄化曲叶病毒病番茄新品种“Ty191”的选育[J].北方园艺,2023(5):157-160.[10]FRASER R S S,GERWITZ A,BETTI L.Deployment of resistance genes:Implications from studies on resistance-bre-aking isolates of tobacco mosaic virus[C]//Proceedings of the Fourth International Plant Virus Epidemiology Wor-kshop.Montpellier:ISPP,1989.[11]王海娟,魏瑶,刘燕,等.番茄种质几种病害抗性基因的分子标记检测[J/OL].分子植物育种,(2021-09-06)[2023-05-05].https://kns.cnki.net/kns8s/defaultresult/index?crossids=YSTT4HG0%2CLSTPFY1C%2CJUP3MUPD.[12]陈昆松,李方,徐昌杰,等.改良CTAB法用于多年生植物组织基因组DNA的大量提取[J].遗传,2004,26(4):529-531.[13]孙亚林.番茄四个抗病基因的基因标记的创建与辅助选择[D].武汉:华中农业大学,2008.[14]李君明,宋燕,陈丽静,等.利用多重PCR反应同时筛选番茄Tm2~2和Mi基因[J].农业生物技术学报,2005,13(6):698-702.[15]SIMONS G,GROENENDIJK J,WIJBRANDI J,et al.Dissection of the Fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy[J].The Plant Cell,1998,10(6):1055-1068.[16]孙功.番茄斑萎病毒抗病基因Sw-5的分子标记及抗病种质筛选[D].石家庄:河北科技大学,2018.[17]蒋振,晋萍,程斐,等.番茄抗性基因Ty-1的PCR快速检测[J].山东农业科学,2014,46(3):5-8,45.[18]JI Y F,van BETTERAY B,SMEETS J,et al.Co-dominant SCAR makers,P6-25,for detection of the Ty-3,Ty-3a,and Ty-3b introgressions from three Solanum chilense at 25 cM of chromosome 6 of begomo virus resistant tomatoes.Rep[J].Tomato Genet Coop,2007,57:25-58.[19]刘放,魏小红,张小芳,等.不同番茄材料黄化曲叶病毒病抗性评价及抗性基因检测[J].西北农业学报,2020,29(12):1905-1912.[20]陈宝玲,甘桂云,王先裕,等.126份番茄材料的抗性基因分子标记检测[J].中国蔬菜,2016(6):34-41.[21]陈莉菁,刘子记,谢尚潜,等.5个抗病基因功能标记在234份番茄材料中的检测[J].热带作物学报,2021,42(6):1685-1693.[22]杨超沙,王珊珊,尹伟平,等.八十一份番茄种质资源的六种病害抗病基因检测[J].北方园艺,2022(20):1-9.[23]张文慧.陕南山区54个番茄地方品种抗病基因鉴定[J].陕西农业科学,2022,68(3):43-45.[24]龚照.抗黄化曲叶病毒病粉果番茄自交系评价和杂交组合的筛选[D].武汉:华中农业大学,2022.

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Last Update: 2024-05-21