|Table of Contents|

Screening,Biocontrol Mechanism of Antagonistic Bacterial Against Gray Mold of Paeonia lactiflora

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年7
Page:
57-66
Research Field:
Publishing date:

Info

Title:
Screening,Biocontrol Mechanism of Antagonistic Bacterial Against Gray Mold of Paeonia lactiflora
Author(s):
LIU Ping; WANG Zuhua; MA Yinhao; WANG Yudie; WEI Daowei; YANG Ruixian
(Department of Environmental Engineering and Chemistry，Luoyang Institute of Science and Technology，Luoyang,Henan 471023)
Keywords:
Paeonia lactiflora; gray mold; endophytic bacteria; Bacillus amyloliquefaciens; bio-control mechanisms
PACS:
S 436.8
DOI:
10.11937/bfyy.20233651
Abstract:
The typical leaves of peony grey mold disease were used as experimental materials，the pathogens were isolated by the tissue isolation method.Plate confrontation method was used to screen endophytic bacteria against peony gray mold pathogen.Based on morphological characteristics and molecular biology identification，the pathogens and antagonistic strains were identified,respectively.By inoculating the isolated leaves，the control efficacy of antagonistic strains on peony gray mold was tested,the types of pathogens causing peony gray mold disease in Luoyang area was clarified and endophytic bacterial strains for controlling peony gray mold disease was obtained,in order to provide reference for the prevention and control of gray mold disease in peony.The results showed that the pathogen causing the gray mold disease of peony in Luoyang area was Botrytis cinerea.Three endophytic bacteria SY5，SY15 and SY30 with significant antagonistic effects against Botrytis cinerea were selected.Among them，strain SY5 had a significant antibacterial effect，with an inhibition rate of 84.44%.The results of the control of isolated leaves of peony showed that all three strains had good control effects on peony gray mold，and the control effect of strain SY5 could reach 67.66%.Strains SY5，SY15 and SY30 were identified as Bacillus amyloliquefaciens.Preliminary exploration of the biocontrol mechanisms of three strains showed that three strains could synthesize lipopeptides and had strong teratogenic effects on the mycelium of Botrytis cinerea.Among them，the lipopeptides of SY5 showed the highest inhibition rate of 88.89%.

References:

［1］姜瑶,葛金涛,宁传龙,等.芍药病害种类及其品种感病性调查［J］.江苏农业科学,2013,41(1):125-127.［2］陈士宁.设施栽培芍药灰霉病和根腐病的初步研究［D］.北京:北京林业大学,2011.［3］蓝莹.芍药灰霉病病原鉴定及生物学的研究［J］.南京林业大学学报(自然科学版),1987,11(1):8-14.［4］马佳,李颖,胡栋,等.芽胞杆菌生物防治作用机理与应用研究进展［J］.中国生物防治学报,2018,34(4):639-648.［5］高芸.生防芽孢杆菌及假单胞菌拮抗植物微生物病害研究进展［J］.北方园艺,2021(2):131-136.［6］SHAFI J,TIAN H,JI M.Bacillus species as versatile weapons for plant pathogens:A review［J］.Biotechnology & Biotechnological Equipment,2017,31(3):446-459.［7］王莉,米佳雯,池明,等.解淀粉芽胞杆菌DS-1菌剂的研制及田间应用效果［J］.植物保护,2020,46(5):64-69.［8］ZABIHULLAH S,杨娜,张静,等.棉花内生解淀粉芽孢杆菌489-2-2对棉花黄萎病的防效研究［J］.核农学报,2021,35(1):41-48.［9］周维,田丹丹,李朝生,等.香蕉内生细菌G9R-3分离鉴定及其拮抗香蕉枯萎病菌活性评价［J］.西南农业学报,2020,33(7):1493-1498.［10］杨瑞先,刘萍,王祖华,等.洛阳市牡丹灰霉病病原菌的鉴定［J］.植物保护学报,2017,44(4):623-629.［11］刘萍,王祖华,张七,等.金黄色葡萄球菌拮抗菌株的筛选鉴定及其抗菌物质分析［J］.微生物学通报,2020,47(5):1544-1551.［12］沈萍,陈向东.微生物学实验［M］.4版.北京:高等教育出版社,2007.［13］王海霞,郑成忠,东保柱,等.燕麦内生细菌YN-J3的分离鉴定及防病促生作用研究［J］.中国生物防治学报,2022,38(2):447-457.［14］吴力红,李润根,廖振军,等.龙牙百合灰霉病病原菌灰葡萄孢菌的分离与鉴定［J］.湖南农业科学,2021(7):7-10.［15］李鸿浩,徐超,林智慧,等.烟草灰霉病菌对3种杀菌剂的敏感性［J］.福建农林大学学报(自然科学版),2021,50(6):741-745.［16］李文艺,李洁维,王发明,等.广西猕猴桃春季病害鉴定［J］.湖北民族大学学报(自然科学版),2021,39(4):367-371.［17］张双艳,孟静,韩洋琳,等.云南地区月季灰霉病病原菌的分离鉴定［J］.浙江大学学报(农业与生命科学版),2019,45(6):692-698.［18］崔蕴刚,张华敏,李延龙,等.韭菜灰霉病病原鉴定及其生物学特性［J］.北方园艺,2020(4):14-19.［19］MUOZ G,CAMPOS F,SALGADO D,et al.Molecular identification of Botrytis cinerea,Botrytis paeoniae and Botrytis pseudocinerea associated with gray mould disease in peonies (Paeonia lactiflora Pall.) in Southern Chile［J］.Revista Iberoamericana De Micologia,2016,33(1):43-47.［20］BLAKE C,CHRISTENSEN M N,KOVCS  T.Molecular aspects of plant growth promotion and protection by Bacillus subtilis［J］.Molecular Plant Microbe Interaction,2021,34(1):15-25.［21］程敏,徐秋芳.解淀粉芽孢杆菌植物亚种CGMCC 11640对山核桃干腐病菌的抑制机制［J］.浙江农林大学学报,2017,34(2):326-331.［22］LYU J,DA R,CHENG Y,et al.Mechanism of antibacterial activity of Bacillus amyloliquefaciens C-1 lipopeptide toward anaerobic Clostridium difficile［J］.BioMed Research International,2020,2020:3104613.［23］敖静,李杨,刘晓辉,等.一株黄瓜枯萎病拮抗细菌的筛选鉴定及抑菌作用初探［J］.云南农业大学学报(自然科学),2022,37(3):429-434.［24］李永丽,源春彦,周洲,等.解淀粉芽孢杆菌菌株P-r21抑菌广谱性及对番茄灰霉病的防治［J］.中国农学通报,2023,39(6):105-110.［25］朱録媛,崔国兵,孙文达,等.甘蔗内生解淀粉芽孢杆菌CGB15的分离、鉴定及生防活性［J］.微生物学报,2022,62(5):1698-1710.［26］张强强,任苗苗,顾沛雯,等.四种复配芽孢菌剂对葡萄霜霉病的防治［J］.北方园艺,2021(6):29-35.

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Last Update: 2024-05-10