|Table of Contents|

Isolation and Identification of Pathogenic Bacteria Causing Postharvest Rot Disease in Green Walnuts

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年4
Page:
74-80
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Pathogenic Bacteria Causing Postharvest Rot Disease in Green Walnuts
Author(s):
ZHANG Qin1; KANG Mingli 1; ZHAO Ru1; ZHU Zhiqiang2; 3; HE Aimin4; JI Yangyang4
(1.School of Food and Biology，Hebei University of Science and Technology,Shijiazhuang，Hebei；2.National Engineering Technology Research Center for Preservation of Agriculture Products (Tianjin)，Key Laboratory of Agricultural Products Storage and Preservation，Ministry of Agriculture and Rural Affairs,Tianjin；3.Institute of Agricultural Products Preservation and Processing Technology，Tianjin Academy of Agricultural Sciences,Tianjin；4.Hebei Lyuling Kangwei food Co.Ltd.,Xingtai,Hebei)
Keywords:
green walnut; postharvest disease; pathogen identification; ITS sequences analysis
PACS:
S 664.1
DOI:
10.11937/bfyy.20233560
Abstract:
Taking green walnut as the test material,pathogenic bacteria were isolated using tissue isolation method,and their pathogenicity was determined based on Koch′s rule.The morphological characteristics of the pathogenic bacteria and rDNA-ITS sequence analysis were combined to identify the types of pathogenic bacteria,in order to provide reference for the prevention and control measures of post harvest rot disease in green walnut.The results showed that the post harvest diseases of green walnut were anthracnose and black spot,and five dominant pathogenic bacteria were isolated.Based on the morphology of the pathogens and rDNA-ITS sequence analysis,it was confirmed that HT73 and HT55 were Colletotrichum siamense,HT12 was Colletotrichum gloeosporioides,HL35 was Fusarium sp,and HP92 was Botryospaeria dothidea.This study identified the main pathogenic bacteria causing green walnut rot disease,which could provide a basis for effectively preventing postharvest rot disease in green walnuts.

References:

［1］丁真真,夏娜,刘艳全,等.两种天然保鲜剂对气调包装青皮核桃贮藏期品质的影响［J］.食品安全质量检测学报,2023,14(2):271-278.［2］赵凡睿.核桃抗氧化肽改善学习和记忆功能障碍的分子机制研究［D］.长春：吉林农业大学,2021.［3］ADARMANABADI S M H H，GILAVAND H K，TAHERKHANI A，et al.Pharmacotherapeutic potential of walnut (Juglans spp.) in age-related neurological disorders［J］.IBRO Neuroscience Reports，2022（14）：1-20.［4］E.Reduction of bitterness in green walnuts by conventional and ultrasound-assisted maceration［J］.Ultrasonics Sonochemistry，2020，66:105094.［5］RON P,AMNON L,LEON A，et al.Postharvest losses of fruit and vegetables during retail and in consumers′ homes：Quantifications，causes，and means of prevention［J］.Postharvest Biology and Technology,2018,139：135-149.［6］林珊宇,朱桂宁,贤小勇,等.广西石漠化地区核桃主要病害调查及病原菌鉴定［J］.西南农业学报,2021,34(10):2158-2166.［7］徐小文,李金仓,海都,等.核桃炭疽菌携带病毒种类鉴定及多样性分析［J］.生物技术通报,2023,39(3):278-289.［8］王一峰,王明霞,赵淑玲,等.贮藏期间不同品种(系)青皮核桃的品质变化［J］.经济林研究,2020,38(3):136-144.［9］OYEDEJI E O,AROGUNDADE O,TAIRU F M,et al.Identification and characterization of fungi pathogen causing fruit rot disease of watermelon (Citrullus lanatus)［J］.Archives of Phytopathology and Plant Protection,2022,55(3):344-354.［10］曲文文.山东省核桃（Juglans regia）主要病害病原鉴定［D］.泰安：山东农业大学,2011.［11］PAN J,MENG J,ZHANG D,et al.Genome sequence resource of Botryosphaeria dothidea strain XNHG241，a causal agent of peach gummosis［J］.Plant Disease,2023,107(7):2205-2208.［12］张海军,陈春艳,谢映平,等.核桃腐烂病病原菌的分离与鉴定［J］.中国植保导刊,2018,38(9):17-20.［13］雷霁卿,曾傲敏,刘颖,等.“贵长”猕猴桃蒂腐病病原菌多样性及致病力测定［J］.北方园艺,2022(21):31-37.［14］毕司进.黑核桃腐烂病病原鉴定和生物特性研究及其防控药剂筛选［D］.乌鲁木齐：新疆农业大学,2019.［15］LIU H,LI Y,LI X,et al.First report of tobacco anthracnose caused by Colletotrichum nymphaeae in China［J］.Plant disease,2023,107(8):2279-2571.［16］NUEZ-GARCA P R，CARRILLO-FASIO J A，MRQUEZ-LICONA G，et al．First report of Colletotrichum tropicale causing anthracnose on pitahaya fruit in Mexico［J］．Plant Disease，2023,107(7):1968-2271.［17］CHRISTERE N M L，MODESTE L S，SEVERIN N T，et al.Molecular identification of fungal pathogens associated with post-harvest yam tubers rot in mbam et kim division (cameroon) with emphasis on Penicillium monomenatosum (Frisvad，Filt.& Wicklow) as a first report［J］.American Journal of Microbiological Research，2020，8(2)：73-78.［18］MEDIC A,SOLAR A,HUDINA M，et al.Phenolic response to walnut anthracnose (Ophiognomonia leptostyla) infection in different parts of juglans regia husks，using HPLC-MS/MS［J］.Agriculture,2021,11(7):659.［19］郜玉钢,魏雪,臧埔,等.天麻萌发菌源病原菌的分离鉴定及其拮抗菌株筛选［J］.北方园艺,2022(20):104-111.［20］王伟超.甘蔗梢腐病病原菌鉴定及其遗传多样性分析［D］.南宁：广西大学,2022.［21］虞凡霜,强遥,秦双林,等.一株侵染黄桃果实的葡萄座腔菌菌株鉴定［J］.江西农业大学学报,2021,43(3):566-573.［22］WANG Y,CHEN J,XIE L X,et al.First report of shoot and leaf blight caused by Botryosphaeria dothidea on Taxus × media in Sichuan province,China［J］.Plant Disease,2023,107(9):2579-2897.［23］薄淑文,喻红稠,覃悦,等.炭疽属植物病原真菌分泌蛋白的预测及比较分析［J］.福建农林大学学报(自然科学版)，2023,52(6):747-754.［24］谢安娜,徐浩飞,张志林,等.致病镰刀菌的研究进展［J］.湖北工程学院学报,2020,40(6):37-41.［25］XIN L，ZHANG R，WANG X，et al.Extracellular and intracellular infection of Botryosphaeria dothidea and resistance mechanism in apple cells［J］.Horticultural Plant Journal,2023,9(2):209-223.［26］汪筱雪,韦继光,杨振德,等.广西西北部核桃真菌性病害调查及核桃炭疽病防治试验［J］.南方农业学报,2018,49(8):1531-1540.［27］CHANG J,ZHAI F Y,ZHANG Y B，et al.Identification and characterization of Colletotrichum fioriniae and C.fructicola that cause anthracnose in pecan［J］.Frontiers in Plant Science,2022(13):1043750.

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Last Update: 2024-03-15