|Table of Contents|

Investigation and Pathogen Identification of Watermelon Virus Disease in Typical Hilly Area of South Taihang

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年11
Page:
35-39
Research Field:
Publishing date:

Info

Title:
Investigation and Pathogen Identification of Watermelon Virus Disease in Typical Hilly Area of South Taihang
Author(s):
LI Xiuqi; JIA Baohua; HAO Haohao; MA Chaoxi; XIAO Xingzhong
(Jiyuan Academy of Agricultural Sciences，Jiyuan，Henan 459002)
Keywords:
watermelon; viral disease; pathogen identification; South Taihang
PACS:
S 651
DOI:
10.11937/bfyy.20233447
Abstract:
The field occurrence and symptoms of watermelon virus disease were used as test materials and were investigated in the open field and small arch shed planted in the typical hilly area of South Taihang，and the pathogen of watermelon virus disease was studied by small RNA sequencing and RT-PCR，in order to provide reference for the development of watermelon virus disease prevention and control strategy in this area.The results showed that the watermelon virus disease in the field generally started from early to middle of May，and the symptoms were mainly mosaic and wrinkling.A total of 7 viruses were detected in 14 disease samples using 16 virus primers.They were watermelon mosaic virus (WMV)，zucchini yellow mosaic virus (ZYMV)，papaya malformed mosaic virus (PLDMV)，melon aphid yeltization virus (MABYV)，watermelon ruffle associated virus 2 (WCLaV2)，watermelon crypto-virus (CiLCV) and melon mixed virus 1 (CMAV1).The detection rate of WMV，CiLCV and CMAV1 virus was 100%，and the detection rate of MABYV virus was the lowest (28.6%).In the field，watermelon virus disease occurred in complex infection，and the disease samples were generally infected with 4-7 different kinds of viruses.Further consistency comparison and analysis of the full-length genomic nucleotide sequence of WMV showed that WMV isolates from this region had high genetic diversity，indicating that the virus may have multiple initial infection sources in this region.

References:

［1］王娟娟,李莉,尚怀国.我国西瓜甜瓜产业现状与对策建议［J］.中国瓜菜,2020,33(5):69-73.［2］张琳,杨艳涛,吴敬学.新形势下中国西瓜甜瓜产业发展的战略思考［J］.北方园艺,2014(19):187-190.［3］杨玉莹,毛世平.河北、河南、山东三省农户绿色防控技术采纳行为研究［J］.北方园艺,2022(10):137-143.［4］尹国红,李秀启,黄利华,等.南太行济源地区西瓜病毒病发生原因及综合防治对策［J］.长江蔬菜,2022(3):58-60.［5］彭斌.中国葫芦科作物病毒的分布、多样性及进化研究［D］.武汉:华中农业大学,2020.［6］宇振荣,杨新民,陈雅杰.河南省南太行地区山水林田湖草生态保护与修复［J］.生态学报,2019,39(23):8886-8895.［7］洪帅.南太行地区精准化旅游扶贫问题的研究［J］.农业与技术,2019,39(23):150-154.［8］马泉来,卫华鹏,王小玉,等.河南省南太行地区土地利用及生态服务价值变化［J］.水土保持通报,2020,40(5):234-241.［9］李秀启,尹国红,郝浩浩等.南太行特色西瓜产业发展现状、存在问题及对策:以南太行地区济源示范区为例［J］.长江蔬菜,2021(22):4-6.［10］KUMAR S,STECHER G,LI M,et al.MEGA X:Molecular evolutionary genetics analysis across computing platforms［J］.Molecular Biology and Evolution,2018,35(6):1547-1549.［11］吴洋,李俊香,彭斌,等.河南、甘肃和新疆西瓜甜瓜病毒检测［J］.中国蔬菜,2017(8):31-35.［12］宋顺华,吴萍,徐荗,等.北京顺义西瓜病毒病的检测［J］.北方园艺,2018(14):13-17.［13］赵立群,邱艳红,张晓飞,等.TaqMan探针法实时荧光定量PCR检测西瓜潜隐病毒［J］.中国农业科学,2021,54(20):4337-4347.

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Last Update: 2024-06-27