|Table of Contents|

Plant Regeneration and Proliferation System Establish of Astragalus membranaceus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年6
Page:
93-100
Research Field:
Publishing date:

Info

Title:
Plant Regeneration and Proliferation System Establish of Astragalus membranaceus
Author(s):
ZUO Min1; LI Guangxin1; ZUO Jingjing1; WANG Huijie1; LEI Wei1; ZHANG Xiujun2
(1.College of Agriculture，Shanxi Agricultural University，Taiyuan，Shanxi 030031;2.Shanxi Yanzhou Agricultural Technology Development Co.Ltd.，Shouyang，Shanxi 030054)
Keywords:
Astragalus mongholicus; callus induction; regenerated plants; proliferation; germplasm resources
PACS:
S 853.75
DOI:
10.11937/bfyy.20233377
Abstract:
The root，leaf and hypocotyl of aseptic seedlings derived from Astragalus membranaceus seed were used as explants，and the growth-regulating hormones 6-BA，2,4-D，NAA were set at different concentrations，the effects of different explants on callus induction rate，in vitro plantlet increment and rooting were studied,in order to provide reference for callus induction，plant differentiation and proliferation of different tissue explants of Astragalus membranaceus.The results showed that root，hypocotyl and leaf were the best explants for callus induction，of which，hypocotyl was the best explants，with the highest callus induction rate of 97.92%，at 2，4-D 1.0 mg· L-1+6-BA 0.5 mg·L-1+NAA 0.5 mg·L-1+sucrose 3.0%，the best medium for plantlet proliferation at 1/2MS+6-BA 1.0 mg·L-1+KT 0.5 mg·L-1+sucrose 3.0%，the highest average differentiation rate of regenerated plantlets at 92.67%，and the optimum medium at 1/2MS+NAA 0.5 mg·L-1+sugar 3.0%.The system of plant regeneration and multiplication of Astragalus mongholicus was established in this experiment，which provided a powerful technical reference for its germplasm resources preservation，rapid seedling breeding and development，which has important popularizing value in the production practice of Astragalus mongholicus.

References:

［1］国家药典委员会.中华人民共和国药典(一部)［S］.北京:化学业出版社,2005.［2］崔彬,李苑松.黄芪多糖的药理研究进展［J］.黑龙江科技信息,2012(15):29.［3］魏廷邦,魏玉杰,杨振华,等.种植密度及氮肥对绿洲区蒙古黄芪有效成分和产量的影响［J］.核农学报,2022,36(8):1664-1675.［4］戴莹,杨世海,赵鸿峥,等.药用植物组织培养中褐化现象的研究进展［J］.中草药,2016,47(2):344-351.［5］杜新刚.中药黄芪化学对照品及质量标准研究［D］.北京:北京林业大学,2006.［6］郭生虎,陈虞超,李明,等.膜荚黄芪愈伤组织诱导及植株再生体系的建立［J］.分子植物育种,2020,18(18):6122-6126.［7］宋新华,梁凤英,许封菁,等.火神山医院新冠肺炎预防经典方中12种主成分的含量测定［J］.海军医学杂志,2022,43(2):166-171.［8］任晋宏,王永辉,薛慧清,等.蒙古黄芪中核糖核酸酶活性蛋白AmPR-10的纯化和性质研究［J］.中草药,2017,48(19):3945-3953.［9］薛鹏举.黄芪优质高产栽培技术［J］.现代农业,2012(10):3.［10］钱丹,黄璐琦,崔光红,等.黄芪种质资源的研究概况［J］.中国实验方剂学杂志,2009,15(3):86-89.［11］李佳,廖智铭,任建利,等.蒙古黄芪愈伤组织诱导及植株再生［J］.湖北农业科学,2016,55(19):5091-5094.［12］秦雪梅,李震宇,孙海峰,等.我国黄芪药材资源现状与分析［J］.中国中药杂志,2013,38(19):3234-3238.［13］霍晓兰,闫敏,孟全业,等.不同激素浓度对黄芪茎段组织培养的影响［J］.山西农业科学,2009,37(3):25-27.［14］王树斌,全雪丽,周玉红,等.膜荚黄芪子叶节植株再生体系的建立［J］.北方园艺,2012(6):177-178.［15］张强,于亚军,贾岭,等.膜荚黄芪(Astragalus membranaceus (fisch) bunge)组织培养再生体系的建立［J］.北京农学院学报,2014,29(3):18-20.［16］包英华,苏格尔.蒙古黄芪的组织培养研究［J］.韶关学院学报(自然科学版),2002，23(9):70-76.［17］田雨,高新新,张秀娟,等.蒙古黄芪与膜荚黄芪愈伤组织诱导及再生植株体系的建立［J］.分子植物育种,2022,20(15):5080-5087.［18］刘德旺,龚苏晓,朱雪瑜,等.蒙古黄芪药材、生饮片及其炮制品质量差异性研究［J］.中草药,2016,47(6):905-910.［19］步营,朱文慧,张宗申,等.黄芪愈伤组织中黄芪多糖含量影响因素的研究［J］.食品科技,2008,33(5):65-68.［20］喻娜,张晓峰,李华清,等.旱半夏不同外植体的愈伤组织诱导［J］.分子植物育种,2018,16(17):5757-5764.［21］庾韦花,石前,张向军,等.提高天门冬种子发芽率及育苗基质筛选研究［J］.种子,2020,39(6):83-86.［22］叶世青.浸种水温和时间对杂交籼稻种子吸水的影响［J］.杂交水稻,2018,33(1):31-33.［23］谭璐娜,彭嘉文,许环宇,等.紫花苜蓿胚性愈伤组织诱导与增殖的研究［J］.草地学报,2023,31(4):1057-1063.［24］FBREGAS N,FORMOSA-JORDAN P,CONFRARIA A,et al.Auxin influx carriers control vascular patterning and xylem differentiation in Arabidopsis thaliana［J］.PLoS Genetics,2015,11(4):e1005183.［25］牛西午,詹海仙,畅志坚,等.不同激素浓度对柠条茎段组织培养及植株再生的影响［J］.华北农学报,2005,20(1):35-37.［26］王浩,孙丽英.植物激素调控丛枝菌根发育的作用机制研究进展［J］.微生物学通报,2022,49(10):4448-4466.［27］XU B,WU R,GAO C,et al.Establishment of tissue culture regeneration system for Medicago ruthenica L.cv.‘zhilixing’［J］.Legume Research-an International Journal,2021(2):45.［28］POPKO J,HNSCH R,MENDEL R R,et al.The role of abscisic acid and auxin in the response of poplar to abiotic stress［J］.Plant Biology,2010,12(2):242-258.［29］IONKOVA I,MOMEKOV G,PROKSCH P.Effects of cycloartane saponins from hairy roots of Astragalus membranaceus Bge.,on human tumor cell targets［J］.Fitoterapia,2010,81(5):447-451.［30］张弘弛,刘瑞,高志慧,等.恒山黄芪毛状根遗传转化体系的建立及活性成分含量测定［J］.北方园艺,2022(9):93-99.［31］银喆,温奇龙,蔡丹昭.三叶青多糖的提取与抗氧化活性的研究［J］.北方园艺,2020(22):96-103.

Memo

Memo:
-
Last Update: 2024-04-11