|Table of Contents|

Cluster Analysis and Fingerprint Construction of 33 Germplasm Resources of Bletilla striata Rchb.f.Based on SRAP

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年5
Page:
97-103
Research Field:
Publishing date:

Info

Title:
Cluster Analysis and Fingerprint Construction of 33 Germplasm Resources of Bletilla striata Rchb.f.Based on SRAP
Author(s):
GUI Tengqin1; 2; WU Zhongliang1; 2; LI Chunyan1; 2; WEI Yan1; 2
(1.School of Biology and Chermistry，Minzu Normal University of Xingyi，Xingyi，Guizhou 562400；2.Research and Development of Biological Resources，Key Laboratory of National Medicine，Xingyi，Guizhou 562400)
Keywords:
SRAP markers; cluster analysis; DNA fingerprints; Bletilla striata Rchb.f.; germplasm resource
PACS:
S 664.1
DOI:
10.11937/bfyy.20233313
Abstract:
A total of 33 wild Bletilla striata Rchb.f.from six different province were studied with 13 pairs of SRAP combining primers，clustering analysis and fingerprint construction of 33 germplasm resources of Bletilla striata Rchb.f.was carried out，in order to provide reference of plant resourceses protection and mixed falsify for the appraisal.The results showed that 13 SRAP combining primers produced 182 bands，included 152 polymorphic bands，with a polymorphism ratio of 83.52%.The average Shannon′s information index and the average gene diversity index were 0.484 6 and 0.324 3.Based on UPGMA，six populations gathered in two clusters.The cluster Ⅰ included three populations.Three populations were respectively taken from Guangdong province，Guangxi province and Hunan province.The another three populations which came from Sichuan province，Guizhou province and Yunan province belonged to the cluster Ⅱ.Mantel test revealed significant positive correlation between genetic distances and geographic distance (r=0.624 6;P＜0.001).The DNA fingerprinted of 33 germplasm resources of Bletilla striata Rchb.f.were constructed by with four core SRAP combining primers.

References:

［1］扬春澍.药用植物学［M］.上海：上海科学技术出版社,1998.［2］国家药典委员会.中国药典［M］.北京：中国医药科技出版社，2015.［3］李慧峰，冉昆，王涛.利用SRAP 标记构建山东省苹果资源指纹图谱［J］.沈阳农业大学学报，2020,51（4）：470-475.［4］郭艳春，张力岚，陈思远，等.黄麻应用核心种质的DNA分子身份证构建［J］.作物学报,2021，47（1）:80-93.［5］靳玲兵.基于SSR标记的核桃不同品种DNA指纹图谱构建及在亲子鉴定中的应用［D］.杨凌：西北农林科技大学，2022.［6］徐宗大,赵兰勇,张玲,等.玫瑰SRAP 遗传多样性分析与品种指纹图谱构建［J］.中国农业科学,2011,44（8）:1662-1669.［7］孙泽硕，蒋冬月，柳新红，等.基于SSR 标记的42份樱花品种的聚类分析及DNA指纹图谱构建［J］.园艺学报，2023，50(3):657-668.［8］王黎明，焦少杰，姜艳喜，等.142份甜高粱品种的分子身份证构建［J］.作物学报，2011，37（11）：1975-1983.［9］秦瑞英，许学，张立平，等.小麦SSR指纹图谱及品种身份证的构建-基于毛细管电泳分析［J］.中国农学通报，2017，33（34）：46-55.［10］李志强，吴超，贺熙勇，等.基于SSR标记的澳洲坚果种质资源DNA指纹图谱的构建［J］.果树学报，2022，39（11）:2028-2035.［11］李超，杨英，陈伟，等.西州密瓜系列甜瓜SSR 指纹图谱构建及聚类分析［J］.园艺学报，2022,49（3）:622-632.［12］郑海燕，粟建光,戴志刚,等.利用ISSR和RAPD标记构建红麻种质资源分子身份证［J］.中国农业科学,2010，43(17):3499-3510.［13］黎君.白及的遗传多样性分析与DNA指纹图谱研究［D］.汉中：陕西理工大学，2017.［14］孙宇龙，侯北伟，耿丽霞，等.基于SRAP 标记的白芨遗传多样性和遗传结构评价［J］.药学学报，2016，51（1）：147-152.［15］桂腾琴，伍伟，卢鹏，等.荷花品种不同部位DNA 提取的比较研究［J］.种子，2016，35(7):41-44.［16］邵雪花，刘传滨，匡石滋，等.橄榄种质资源遗传多样性分析及指纹图谱构建［J］.江苏农业科学，2023，51(5):94-102.［17］黄健婷，莫怿，陶亮，等.基于EST-SSR 分子标记的澳洲坚果分子指纹图谱构建［J/OL］.基因组学与应用生物学,(2023-03-16)［2023-09-01］.https://kns.cnki.net/kcms/detail/45.1369.Q.20230315.1544.002.html.［18］NIU J，ZHAO G，MI Z，et al.Denovo sequencing of Bletilla striata (Orchidaceae) transcriptme and identification of genes involved in polysaccharide biosynthesis［J］.Genetics and Molecularbiology，2020,43(3):136-141.［19］黎君，杨恒，周天华.白芨SSR 引物筛选及群体遗传多样性研究［J］.西北植物学报，2016，36（7）:1343-1350.［20］HU D C，ZHANG P，HAO W.SRAP analysis on the genetic relationships of 24 mulberry (Morus L.) accessions in the lower regions of the Yellow River［J］.Genet Resour Crop Evol，2015，62（1）:13-19.［21］LI G，QURIOS C F.Sequence related amplified polymorphism(SRAP) a new marker system based on a simple PCR reaction:Its application to mapping and gene tagging in Brassica［J］.Theoretical and Applied Genetics，2001,103:455-461.［22］CAI X Y，FENG Z Y，ZHANG X X，et al.Genetic diversity and population structure of an endangered Orchid (Drndrobium loddigesii Rolfe) from China revealed by SRAP markers［J］.Science Horticulture，2011，129:877-881.［23］王晶，李建，史根生，等.利用SRAP 分子标记研究山西省野生山丹的遗传多样性［J］.山西农业大学学报（自然科学版），2020,40（6）：46-53.［24］蒋小刚，王华，张美德.白术种质资源遗传多样性的SRAP分析［J］.北方园艺，2022（1）：109-115.［25］HAJARJ E，HANWEG K，NONYANE D，et al.Sex determination in Kei-apple(Dovyalis caffra)using molecular markers and flow cytometry［J］.South African Journal of Botany，2019(127):278-283.［26］李佳奇，于卓，杨东升，等.基于SRAP分子标记的冰草遗传连锁图谱构建［J］.西北植物学报，2019,39（1）：76-83.［27］王忠华.DNA 指纹图谱技术及其在作物品种资源中的应用［J］.分子植物育种，2006，4（3）:425-430.［28］高源，王昆，王大江，等.利用TP-M13-SSR标记构建苹果栽培品种的分子身份证［J］.园艺学报，2016,43（1）：25-37.［29］HUANG X F,WANG K,WANG D J，et al.Development and genetic diversity analysis of genomic SSR markers in Lyciumrutenicum Murr［J］.Journal of Northwest A&F University (Natural Science),2016,43（1）:25-37.

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Last Update: 2024-03-28