|Table of Contents|

Genetic Diversity and Population Structure of Different Populations in Artemisia argyi Germplasm

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年2
Page:
94-100
Research Field:
Publishing date:

Info

Title:
Genetic Diversity and Population Structure of Different Populations in Artemisia argyi Germplasm
Author(s):
XU Lanjie; YANG Qing; YU Yongliang; AN Sufang; DONG Wei; LIANG Huizhen
(Institute of Chinese Herbel Medicines，Henan Academy of Agricultural Sciences，Zhengzhou,Henan 450002)
Keywords:
Artemisia argyi germplasm; population; genetic diversity; population structure
PACS:
R 282.2
DOI:
10.11937/bfyy.20232723
Abstract:
Twelve populations of Artemisia argyi and 22 SCoT primers were used as test material，the genetic diversity and population structure characteristics of the 12 populations were studied by methods of NTSYS-pc 2.1,Popegen 1.32,in order to provide reference for the breeding of new Ai varieties.The results showed that 87 alleles were detected by 22 SCoT markers，with an average of 3.95 alleles for each primers.The average PIC，I and H were 0.690，0.483 and 0.317 that showed high genetic diversity.The average value of Na，Ne，I and He per locus were 1.444，1.419，0.349 and 0.238，Anyang Tangyin population was the highest.Genetic similarity and the genetic distance were 0.795-0.975 and 0.025-0.229，with average of 0.909 and 0.097.Jiyuan and Huanggang Qichun populations had the highest and nearest genetic similarity coefficient and genetic distance.Gst of SCoT was 0.293，indicated a low level of genetic diversity among populations，and genetic differences for 9% was attributable to inter-population.Nm was 2.022 among 12 populations indicated that gene flow was lower among populations，resulting genetic difference was small among populations.Four classes were clustered at the genetic similarity coefficient of 0.914 including 8，2，1 and 1 population，respectively.

References:

［1］聂韡，刘畅，单承莺.艾草的本草考证及资源分布［J］.中国野生植物资源，2019，38(4):93-95,105.［2］庾韦花，石前，张向军，等.广西不同产地艾挥发性成分的分析［J］.食品工业，2020，41(12):310-312.［3］董鹏鹏，梅全喜，戴卫波.不同产地艾叶总黄酮、重金属和硒元素的含量比较研究［J］.时珍国医国药，2016，27(1):74-76.［4］李超，杨成敏，高丽，等.不同表型艾叶中矿物元素和主要药用成分差异分析［J］.河南农业科学，2021，50(4):48-55.［5］国家药典委员会.中华人民共和国药典(一部)［M］.北京：中国医药科技出版社，2020.［6］何柳，王云鹏，谢卫红，等.艾叶水提物和酸提物的抗氧化及抗菌活性比较［J］.现代食品科技，2021，37(10):205-213.［7］谢利，刁本恕，刁灿阳，等.扶正减毒抗癌方联合艾灸对非小细胞肺癌放射治疗增效减毒作用的临床研究［J］.辽宁中医杂志，2016，43(4):762-764.［8］黄亚蓉,耿文,李清,等.针灸对类风湿关节炎的免疫调节作用研究进展［J］.国际中医中药杂志,2022,44(1):114-121.［9］詹振吉,王健.艾灸对机体免疫系统的影响［J］.中医学报,2016,31(3):449-452.［10］赵博宇，董诚明.河南艾不同种质资源形态特征比较［J］.中医学报，2019，34(5):1024-1029.［11］陈昌婕，罗丹丹，苗玉焕，等.基于农艺性状和叶片表型性状的艾种质资源多样性分析［J］.中国中药杂志，2021，46(11):2773-2782.［12］陈昌婕，罗丹丹，苗玉焕，等.艾种质资源挥发性成分分析与评价［J］.中国中药杂志，2021，46(15):3814-3823.［13］陈昌婕，罗丹丹，苗玉焕，等.不同艾种质资源叶片品质的分析与评价［J］.中国实验方剂学杂志，2021，27(9):129-136.［14］陈昌婕，苗玉焕，方艳，等.不同艾种质资源矿质元素含量及其与品质的关系［J］.中国中药杂志，2022，47(4):880-888.［15］许兰杰，梁慧珍，余永亮，等.基于农艺和品质性状的宛艾种质综合评价［J］.河南农业科学，2022，51(8):55-56.［16］FENG S G，HE R F，JIANG M Y，et al.Genetic diversity and relationships of medicinal Chrysanthemum morifolium revealed by start codon targeted (SCoT) markers［J］.Scientia Horticulturae，2016，201(30):118-123.［17］卢家仕，李先民，黄展文，等.基于SCoT分子标记的金花茶组植物种质资源遗传多样性分析［J］.中草药，2021，52(20):6357-6364.［18］尚小红，严华兵，曹升，等.广西地方葛根种质资源遗传多样性的SCoT分析［J］.核农学报,2019,33(7):1311-1317.［19］王惠君，卢诚，黎明，等.传统中药艾种质资源的ISSR遗传多样性分析［J］.贵州农业科学，2015，43(8):1-5.［20］COLLARD B C Y，MACKILL D J.Start codon targeted (SCoT) polymorphism：A simple，novel DNA marker technique for generating gene-targeted markers in plants［J］.Plant Molecular Biology Reporter，2009，27(1)：86-93.［21］冯俊彦，康乐，郎涛，等.基于SCoT分子标记的甘薯及其野生种遗传多样性分析［J］.华北农学报，2021，36(1):18-26.［22］王梦梦,孙德岭,陈锐,等.花椰菜核心种质的构建与评价［J］.园艺学报,2023,50(2):421-431.［23］张瑜,韦廷舟,邹大方，等.白芨SCoT分子标记技术体系的建立及其在快繁苗遗传稳定分析中的应用［J］.北方园艺，2018(4):152-159.［24］查美琴.新疆枸杞和枣种质资源遗传多样性分析［D］.乌鲁木齐：新疆农业大学，2016.［25］刘新梅，梁慧珍，许兰杰，等.红花SCoT-PCR反应体系的建立与优化［J］.分子植物育种，2022，20(3):895-901.［26］李文秀，李进良，贺军军，等.基于SSR标记的砂仁种质资源遗传多样性与群体结构分析［J］.中国中药杂志，2022，47(17):4618-4626.［27］LIU J Q，DUAN Y W，HAO G，et al.Evolutionary history and underlying adaptation of alpine plants on the Qinghai-Tibet Plateau［J］.Journal Sytem Evol，2014，52(3):241-249.

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Last Update: 2024-02-08