|Table of Contents|

Isolation and Identification of Pathogens Causing Blueberry Rot During Postharvest Storage at Low Temperature and Screening of Biological Fungicides

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年3
Page:
85-92
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Pathogens Causing Blueberry Rot During Postharvest Storage at Low Temperature and Screening of Biological Fungicides
Author(s):
LUO Donglan1QU Guangfan1LI Jiangkuo2ZHANG Yu1YE Shengjie1CAO Sen1
(1.Guizhou Engineering Research Center for Fruit Processing,Guiyang College,Guiyang,Guizhou 550005;2.Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products/Key Laboratory of Storage of Agricultural Products,Ministry of Agriculture and Rural Affairs/National Engineering and Technology Research Center for Preservation of Agricultural Products (Tianjin),Tianjin 300384)
Keywords:
blueberry fruitpathogenetic fungiisolation and identificationbiological fungicide
PACS:
S 663.9
DOI:
10.11937/bfyy.20232685
Abstract:
Blueberry fruits(‘Powder Blue’) were used as experimental material,tissue isolation method,Koch′s law,morphological identification and rRNA-ITS sequence homology analysis methods were used to study the main rot-causing pathogen species of blueberry fruits and to screen for suitable high-efficiency and low-toxicity biocides,in order to provide reference for the prevention and control of berry fruits postharvest diseases.The results showed that the pathogens causing blueberry rot during low-temperature storage were Botrytis cinerea,Alternaria alternata,Colletotrichum gloeosporioides,Neopestalotiopsis clavispora and Neofusicoccum parvum.For the first time,Colletotrichum gloeosporioides was found to be the dominant pathogen causing fruit rot of ‘Powder Blue’ blueberry.The indoor toxicity test showed that ethylicin showed strong antibacterial activity against Colletotrichum gloeosporioides,with EC50 value of 123.16 mg·mL-1,followed by matrine,zhongshengmycin,kasugamycin and eugenol,with EC50 value of 231.23,519.55,546.29,865.26 mg·mL-1 respectively.Therefore,ethylicin could be used as an effective biocide for field control of blueberry in the later stage.

References:

[1]LI D L,LI B,MA Y,et al.Polyphenols,anthocyanins,and flavonoids contents and the antioxidant capacity of various cultivars of highbush and half-high blueberries[J].Journal of Food Composition and Analysis,2017,62:84-93.[2]吴超,杨富雁,郜帮丽.壳聚糖、山梨酸钾和ε-聚赖氨酸对蓝莓的复合涂膜保鲜效果[J].北方园艺,2022(7):99-105.[3]HOSKIN R T,XIONG J,ESPOSITO D A,et al.Blueberry polyphenol-protein food ingredients:The impact of spray drying on the in vitro antioxidant activity,anti-inflammatory markers,glucose metabolism and fibroblast migration[J].Food Chemistry,2019,280:187-194.[4]阳秀莲,王伟,林树花,等.蓝莓贮藏期病原菌的分离鉴定及植物粗提物抑菌效果研究[J].食品科技,2018,43(5):41-48.[5]王哲捷,徐十,王永平.贵州水果产业发展现状及高质量发展对策[J].北方园艺,2022(6):137-144.[6]ANGELETTI P,CASTAGNASSO H,MICELI E,et al.Effect of preharvest calcium applications on postharvest quality,softening and cell wall degradation of two blueberry (Vaccinium corymbosum) varieties[J].Postharvest Biology and Technology,2010,58(2):98-103.[7]吴文能,雷霁卿,李江阔,等.蓝莓采后病害病原菌分离鉴定及抑菌药剂筛选[J].北方园艺,2019(7):58-64.[8]王丹,张静,贾晓曼,等.蓝莓采后主要病原菌的分离鉴定及肉桂精油抑菌效果[J].食品科学,2019,40(24):167-172.[9]郜海燕,肖尚月,陈杭君,等.蓝莓采后主要病原真菌的分离鉴定与生物学特性研究[J].农业机械学报,2017,48(5):327-334.[10]崔学强,李秀玲,范继征,等.兜兰萎蔫病病原菌的分离与鉴定[J].西南农业学报,2020,33(12):2846-2851.[11]瞿光凡,巴良杰,雷霁卿,等.茉莉酸甲酯对脆红李采后病原菌的抑制作用[J].食品研究与开发,2022,43(24):95-101.[12]姜莉莉,王钦超,宗晓娟,等.草莓果实病原菌的分离鉴定及对12种杀菌剂的敏感性[J].山东农业科学,2022,54(2):129-133.[13]FU X D,LI X A,ALI M,et al.Methionine sulfoxide reductase B5 plays vital roles in tomato fruit defense response against Botrytis cinerea induced by methyl jasmonate[J].Postharvest Biology and Technology,2023,196:112165.[14]FAN L,WEI Y Y,CHEN Y,et al.Epinecidin-1,a marine antifungal peptide,inhibits Botrytis cinerea and delays gray mold in postharvest peaches[J].Food Chemistry,2023,403:134419.[15]JIANG B,LIU R L,FANG X J,et al.Botrytis cinerea infection affects wax composition,content and gene expression in blueberry fruit[J].Postharvest Biology and Technology,2022,192:112020.[16]WANG L Y,HU J P,LI D S,et al.Isolation and control of Botrytis cinerea in postharvest green pepper fruit[J].Scientia Horticulturae,2022,302:111159.[17]JAVANMARDI Z,KOUSHESH S M,NOURBAKHSH H,et al.Efficiency of nanoemulsion of essential oils to control Botrytis cinerea on strawberry surface and prolong fruit shelf life[J].International Journal of Food Microbiology,2023,384:109979.[18]LIU Y,LEI X M,DENG B,et al.Methionine enhances disease resistance of jujube fruit against postharvest black spot rot by activating lignin biosynthesis[J].Postharvest Biology and Technology,2022(190):111935.[19]LI Y C,MA Y Y,ZHANG T T,et al.Exogenous polyamines enhance resistance to Alternaria alternata by modulating redox homeostasis in apricot fruit[J].Food Chemistry,2019,301(15):125303.[20]向妙莲,吴帆,李树成,等.外源褪黑素调控活性氧代谢诱导梨果实抗采后黑斑病[J].园艺学报,2022,49(5):1102-1110.[21]吴玉珠,陈娜,胡军华,等.青脆李枯萎病致病菌的分离鉴定及药剂筛选[J].湖南农业大学学报(自然科学版),2022,48(3):298-304.[22]王正贤.广西玉林市番石榴果实病害的病原菌鉴定及黑斑病菌的生物学特性研究[D].南宁:广西大学,2021.[23]吴望,吴玉珠,胡军华,等.‘沃柑’果斑病病原菌鉴定及防治药剂筛选[J].果树学报,2021,38(3):385-393.[24]CHEN X Y L,HE B Y,LI H X,et al.First report of black rot on walnut fruits caused by Neofusicoccum parvum in China[J].Plant Disease,2019,103(12):3275.[25]ZHOU D B,JING T,CHEN Y F,et al.Biocontrol potential of a newly isolated Streptomyces sp.HSL-9B from mangrove forest on postharvest anthracnose of mango fruit caused by Colletotrichum gloeosporioides[J].Food Control,2022,135:108836.[26]EKABOTE S D,NARAYANSWAMY P.Bio-efficacy of various fungicides against Clletotrichum gloeosporioides causing anthracnose on pomegranate fruit[J].Acta Horticulturae,2019,1254:253-256.[27]徐成楠,王亚南,胡同乐,等.蓝莓炭疽病病原菌鉴定及致病性测定[J].中国农业科学,2014,47(20):3992-3998.[28]张国辉,刘德波,宋盛英,等.黔东南州蓝莓叶部病害的种类调查和病原鉴定[J].中国森林病虫,2017,36(2):42-46.[29]刘洪坤.蓝莓炭疽病的药剂防治试验[J].中国南方果树,2017,46(2):163-165.[30]张伟,于云政,刘洁,等.生物药剂对花生叶斑病的防治研究[J].花生学报,2016,45(2):62-67.[31]胡容平,石军,林立金,等.猕猴桃溃疡病高效防治药剂的筛选及田间应用评价[J].中国农学通报,2020,36(6):112-116.[32]李慧,刘保军,吴琼,等.3种生防制剂对棉花红腐病和立枯病的防效评价[J].新疆农业科学,2020,57(4):694-704.[33]赵岩,白田田,顾欣,等.宁夏地区欧李根癌病病原菌鉴定及防治药剂筛选[J].东北林业大学学报,2023,51(3):130-135.

Memo

Memo:
-
Last Update: 2024-03-01