|Table of Contents|

Establishment of Sterile Propagation System of Pinellia ternata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年1
Page:
113-120
Research Field:
Publishing date:

Info

Title:
Establishment of Sterile Propagation System of Pinellia ternata
Author(s):
CHEN Guizhi1; LA Guixiao2; GUO Junqi3; SU Xiuhong1; 4; DONG Chengming1; 4; CHEN Suiqing1; 4
(1.College of Pharmacy,Henan University of Chinese Medicine，Zhengzhou,Henan 450046；2.Industrial Crop Research Institute，Henan Academy of Agricultural Sciences，Zhengzhou,Henan 450002；3.Qinyang Agricultural Technology Promotion Center,Qinyang,Henan 454550;4.Henan Synergistic Innovation Center of Respiratory Disease Diagnosis and New Dryg Development，Zhengzhou,Henan 450046)
Keywords:
Pinellia ternata; pant growth regulator; current stimulation; adventitious buds; rooting
PACS:
S 567.239
DOI:
10.11937/bfyy.20232531
Abstract:
The tubers of Pinellia ternata were used as experimental materials.The effects of different types and concentrations of plant growth regulators on adventitious bud induction，proliferation，rooting and the effect of current stimulation on adventitious bud proliferation were studied by tissue culture，in order to provide reference for the establishment of asexual rapid propagation system for Pinellia ternata.The results showed that the optimal medium for adventitious bud induction was MS+1.5 mg·L-1 6-BA+0.1 mg·L-1 NAA.The average number of adventitious buds per explant was 9.38 and the induction rate was 93.75%.The use of current stimulation not only increased the proliferation of adventitious buds，but also significantly promoted the growth of Pinellia ternata plants.Especially under the treatment of 20 mA and 5 minutes，the maximum proliferation of adventitious buds was 13.31 times，and the tissue culture seedlings grew robust and rooted.The optimum induction medium for rooting was 1/2 MS+0.2 mg·L-1 NAA，the rooting rate was 100%，and the number of rooting was 23.75.Asexual rapid propagation system of Pinellia ternata was established to provide reference for high-quality seedling breeding and factory production.

References:

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Last Update: 2024-01-25