|Table of Contents|

Genetic Diversity and Population Structure Analysis of Ganoderma lucidum Based on ITS and ISSR Markers

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年3
Page:
103-111
Research Field:
Publishing date:

Info

Title:
Genetic Diversity and Population Structure Analysis of Ganoderma lucidum Based on ITS and ISSR Markers
Author(s):
ZHAO Cuimin; ZHAO Cen; ZHANG Lanying; WU Qianqian; WU Ensi; LI Minmin
(Liaocheng Academy of Agricultural Sciences，Liaocheng，Shandong 252000)
Keywords:
Ganoderma lucidum; internal transcribed spacer (ITS); inter-simple sequence repeats(ISSR); genetic diversity
PACS:
S 567.3+1
DOI:
10.11937/bfyy.20232329
Abstract:
48 Ganoderma lucidum germplasm resources from different geographical regions were used as samples，and the genetic diversity of Ganoderma lucidum was studied by using ITS and ISSR molecular markers，in order to better display the genetic diversity of different Ganoderma lucidum strains by using genetic information data.The results showed that 48 strains of Ganoderma were divided into Ganoderma lucidum (35)，Ganoderma resinaceum (11)，Ganoderma sichuanense (1) and Trametes cubensis(1) by ITS sequence amplification and sequencing.A total of 53 bands were amplified by 5 ISSR primers，the percentage of polymorphic bands was 96.23%，the Nei′s gene diversity was 0.27，the Shannon′s information index was 0.43.The genetic similarity coefficient of ISSR marker was about 0.70，and 48 strains of Ganoderma were divided into three groups.The first group was 11 strains of Ganoderma lucidum，the second group was 29 ‘Penjing 1’，and the remaining strains were the third group，indicated that the combination of two markers could explain the genetic relationship between different strains in more detail.Strains 16 and 17 were both Ganoderma lucidum，and the genetic similarity coefficient of ISSR molecular markers reached 0.98，suggested that they might be the same variety，which was a synonym phenomenon in production.The ISSR primers selected for PCR amplification in this study had good polymorphism and high stability，which could effectively distinguish sessile Ganoderma lucidum from other Ganoderma lucidum，and reveal the genetic diversity and population genetic structure of the germplasm，which could provide reference basis for the protection of Ganoderma lucidum germplasm resources and the selection of high-quality germplasm materials，so as to better promote the healthy development of Ganoderma lucidum industry.

References:

［1］RAMADAN H A I，NABIH A.Biological identifications through DNA barcodes［M］.Biodiversity conservation and utilization in a diverse world.IntechOpen，2012.［2］胡佳,戴丹,彭新红,等.灵芝属9个野生菌株的分子鉴定与系统发育关系分析［J］.食用菌，2023，45(1)：29-34.［3］谭秀梅,阿地力·沙塔尔,朴春根,等.无柄灵芝遗传多样性的SRAP、ITS、TEF1-α和LSU分析［J］.微生物学通报,2016,43(12):2667-2677.［4］耿晓进,蒋秋梦,张腾洋,等.秦皇岛地区三种野生食用菌分子鉴定与系统发育分析［J］.北方园艺，2021(8)：111-116.［5］孟虎,孙国琴,睢韡,等.ISSR技术在食用菌研究上的应用［J］.北方园艺,2016(5):207-210.［6］陆欢,尚晓冬,宋春艳,等.基于ISSR标记和农艺性状的金顶侧耳种质资源遗传多样性分析［J］.食用菌学报，2022，29(6)：14-24.［7］吕锐玲,陈实,王杰,等.基于ISSR标记的我国羊肚菌栽培品种遗传多样性分析［J］.北方园艺，2023(8)：107-114.［8］郭金英,史灵燕,张慧,等.冀中南毛木耳栽培菌株的鉴定和优良菌株筛选［J］.北方园艺，2019(14)：129-134.［9］李慧,夏伟伟,胡子钰,等.利用ISSR-SCAR标记鉴定金针菇双-单杂交子［J］.北方园艺,2022(16):121-127.［10］KUMAR S,STECHER G,TAMURA K.MEGA7：Molecular evolutionary genetics analysis version 7.0 for bigger datasets［J］.Molecular Biology and Evolution，2016，33(7)：1870-1874.［11］ROHLF F J.NTSYS-pc：Microcomputer programs for numerical taxonomy and multivariate analysis［J］.The American Statistician，1987，41(4)：330.［12］CHEN C,CHEN H,ZHANG Y,et al.TBtools:an integrative toolkit developed for interactive analyses of big biological data［Z］.Molecular Plant,2020,13(8):1194-1202.［13］邢佳慧.灵芝属的物种多样性、分类与系统发育研究［D］.北京:北京林业大学，2019.［14］戚元成,张小强,高玉千,等.中国栽培糙皮侧耳品种体细胞不亲和性实验与RAPD分析结果的比较［J］.菌物学报，2010，29(3)：379-388.［15］袁滨,柯丽娜,黄艺宁,等.应用拮抗和ISSR方法综合鉴定灰树花菌株［J］.福建农业学报，2019，34(4)：393-399.［16］汪乔,王祥锋,郭玉峰,等.肺形侧耳菌株的遗传差异分析与农艺性状评价［J］.中国农学通报，2022，38(10)：46-52.［17］唐传红,唐庆九,张劲松,等.基于rDNA ITS序列和ERIC-PCR研究虫草真菌无性分离物的遗传多样性［J］.南京农业大学学报，2014，37(2)：146-152.［18］XING X K,MA X T,HART M M,et al.Genetic diversity and evolution of Chinese traditional medicinal fungus Polyporus umbellatus (Polyporales，Basidiomycota)［J］.PLoS One，2013，8(3)：e58807.［19］张宇,黄国弟,莫永龙,等.CDDP结合SRAP标记分析杧果品种的遗传多样性［J］.中国南方果树，2022，51(2)：57-63.

Memo

Memo:
-
Last Update: 2024-03-01