|Table of Contents|

Effects of BoEPXA2 Gene on Seed Germination of Kale Under Abiotic Stress

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年1
Page:
60-66
Research Field:
Publishing date:

Info

Title:
Effects of BoEPXA2 Gene on Seed Germination of Kale Under Abiotic Stress
Author(s):
YANG Weimin1; YAN Junfeng1; ZHAI Yuanyuan2; SUN Nan2; WANG Mingcheng2; LI Hongyan2
(1.Garden Center，Huanghuai University，Zhumadian，Henan 463000;2.School of Biotechnology and Food Engineering，Huanghuai University，Zhumadian，Henan 463000)
Keywords:
abiotic stress; seed germination; enzyme activity; tolerance; kale
PACS:
S 635.1
DOI:
10.11937/bfyy.20232159
Abstract:
The wild type，BoEXPA2 gene overexpression，and BoEXPA2 gene suppressed expression of kale were used as experimental materials.Three inorganic reagents，NaCl，sucrose，and mannitol were used.Low and high concentrations of NaCl (100 mmol·L-1 and 200 mmol·L-1)，sucrose (150 mmol·L-1 and 250 mmol·L-1)，and mannitol (200 mmol·L-1and 400 mmol·L-1) groups were set，respectively.The seed germination of three strains under different stresses was analyzed，and antioxidant enzyme activity，MDA content，and BoEXPA2 expression were measured for each genotype under different stresses,in order to explore the effect of BoEXPA2 gene on seed germination of kale under abiotic stress.The results showed that under abiotic stress，compared with wild type seeds，the germination rate of BoEXPA2 inhibited expression seeds was significantly reduced，while the germination rate of BoEXPA2 overexpression lines was significantly increased.Under drought stress and salt stress，the activities of superoxide dismutase (SOD)，peroxidase (POD)，catalase (CAT) and the content of malondialdehyde (MDA) in the seeds of the three genotypes were increased，but the activities of SOD，POD，CAT in the seeds of the BoEXPA2 overexpression strain were far higher than those of the control and suppression expression strains，while the activities of MDA were lower than those of the control and suppression expression strains.When the seeds were treated with NaCl，sucrose and mannitol，the expression of BoEXPA2 decreased with the increase of their content.The results indicated that abiotic stress inhibits the expression of BoEXPA2 in germinated seeds.

References:

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Last Update: 2024-01-22