|Table of Contents|

Identification of Pathogen and Screening of Controlling Agents for Gray Mold Disease of Epimedium sagittatum （Sieb.et Zucc.） Maxim.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年4
Page:
89-95
Research Field:
Publishing date:

Info

Title:
Identification of Pathogen and Screening of Controlling Agents for Gray Mold Disease of Epimedium sagittatum （Sieb.et Zucc.） Maxim.
Author(s):
WANG Xiaojie1; 2; HOU Wenchuan1; 2; YANG Linlin1; 2; DONG Chengming1; 2; ZHONG Hua3; CHU Leixia1; 2
（1.School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou，Henan 450046；2.Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials,Zhengzhou，Henan 450046;3.Pingyu County Rural Agriculture Bureau,Zhumadian，Henan 463400）
Keywords:
Epimedium sagittatum（Sieb.et Zucc.）Maxim.; gray mold disease; identification of pathogenic bacteria; drug screening
PACS:
S 435.67
DOI:
10.11937/bfyy.20231968
Abstract:
The diseased plants of Epimedium sagittatum in the standardized planting base of Epimedium sagittatum in Zhumadian City，Henan Province were used as experimental materials.The tissue separation method was carried out to isolate and purify pathogenic bacteria.Pathogen was identified by pathogenicity testing，morphological identification，and combined phylogenetic analysis based on ITS，PRB2 and HSP60 genes for molecular biology identification.The drug containing tablet method was used to screen the prevention and controlling agents.The types of pathogenic bacteria and efficient control agents of Epimedium sagittarius gray mold disease were studied，in order to provide reference for the prevention and control of Epimedium sagittarius gray mold disease.The results showed that the pathogen of leaf gray mold of Epimedium sagittatum was Botrytis cinerea.Oxime bacteria · tebuconazole，Flusilazole，Pythapyr，Azimeramide，and Fludioxonil all had strong inhibitory effects on the growth of pathogen.Among them，Oxime bacteria · tebuconazole had the best inhibitory effect on pathogenic bacteria with the lowest EC50 value.

References:

［1］周晓洁，姚辛敏，周妍妍.淫羊藿的药理作用研究进展［J］.中医药学报，2022，50(11)：112-115.［2］华辞怡，史有阳，谢颖，等.淫羊藿有效成分治疗乳腺癌作用机制的研究进展［J］.中草药，2022，53(20)：6593-6600.［3］齐芸，郑永先.淫羊藿苷抗肿瘤作用机制研究进展［J］.中医药导报，2022，28(8)：153-157.［4］崔欣萍，闫滨滨，万修福，等.药用淫羊藿栽培研究现状与展望［J］.中国现代中药，2022，24(4)：705-714.［5］肖姬玲，熊毅，梁志怀，等.尖孢镰刀菌F-1对草莓灰霉病防控效果及机制初探［J］.中国生物防治学报，2022，38(4)：840-845.［6］贾爽爽，黄晓庆，孔繁芳，等.黑龙江和云南葡萄产区灰霉病菌对多菌灵和异菌脲的抗性检测［J］.植物保护，2021，47(4)：239-243.［7］黄鑫，郑丽宁，顾学虎，等.生防菌D25与嘧环·咯菌腈复配对番茄灰霉病防治的增效作用［J］.中国生物防治学报，2022，38(4)：860-867.［8］陈炳蔚，李勇，王蓉，等.我国人参灰霉病菌对3种化学杀菌剂的抗药性［J］.中国现代中药，2021，23(12)：2115-2119.［9］ZHANG D，GAO F，JAKOVLIC〖JX-+0.8mm〗〖KG-*2〗＇I，et al.PhyloSuite：An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies［J］.Mol Ecol Resour，2020，20(1)：348-355.［10］WEIR B S，JOHNSTON P R，DAMM U.The Colletotrichum gloeosporioides species complex［J］.Stud Mycol，2012，73(1)：115-180.［11］STAATS M，van BAARLEN P，VAN KAN J A.Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity［J］.Mol Biol Evol，2005，22(2)：333-346.［12］张双艳，孟静，韩洋琳，等.云南地区月季灰霉病病原菌的分离鉴定［J］.浙江大学学报(农业与生命科学版)，2019，45(6)：692-698.［13］LAND M，HAUSER L，JUN S R，et al.Insights from 20 years of bacterial genome sequencing［J］.Funct Integr Genomics，2015，15(2)：141-161.［14］FERRADA E E，LATORRE B A，ZOFFOLI J P，et al.Identification and characterization of Botrytis blossom blight of japanese plums caused by Botrytis cinerea and B.prunorum sp.nov.in Chile［J］.Phytopathology，2016，106(2)：155-165.［15］金华，杨相波，植汉成，等.淫羊藿病虫害及防治［J］.农技服务，2017，34(5)：8-9.［16］韩冲冲.戊唑醇·肟菌酯在水稻群体内的沉积分布及其在水稻环境中的残留行为［D］.南昌：江西农业大学，2019.［17］张正炜，陈秀，石小媛，等.我国农作物灰霉病杀菌剂的应用现状［J］.中国蔬菜，2021(2)：41-46.［18］唐涛，王帆帆，段媛媛，等.13种生物源杀菌剂对华重楼灰霉病的防治［J］.农药，2021，60(4)：297-300.［19］郭成瑾，陈杭，王喜刚，等.育苗基质添加复配微生物菌剂对番茄生长及根腐病发生的影响［J］.北方园艺，2022(16)：52-57.

Memo

Memo:
-
Last Update: 2024-03-15