|Table of Contents|

Cloning and Functional Analysis of [STBX]MIR156c[STBZ] Gene in Chrysanthemum morifolium

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年20
Page:
53-60
Research Field:
Publishing date:

Info

Title:
Cloning and Functional Analysis of [STBX]MIR156c[STBZ] Gene in Chrysanthemum morifolium
Author(s):
HU LeiCHEN XiLIU ZhenXIANG JingHUANG Zhigang
(College of Bioscience and Biotechnology,Hunan Agricultural University/Hunan Provincial Key Laboratory of Phytohormones and Growth Development,Changsha,Hunan 410128)
Keywords:
ChrysanthemumMIR156cgene cloningfunctional analysis
PACS:
S 682.1+1
DOI:
10.11937/bfyy.20231731
Abstract:
Taking Chrysanthemum ‘Jinba’ and Arabidopsis thaliana as experimental materials,the precursor sequence and secondary structure,evolutionary relationship,expression pattern,growth and development function of Chrysanthemum MIR156c gene were studied by bioinformatics and molecular biology methods,in order to provide reference for the molecular mechanism of Chrysanthemum growth and development.The results showed that the sequence length of the cloned Chrysanthemum MIR156c was 212 bp.cmio-MIR156c could form a typical stem-loop structure,which was specific at the 11th position of the mature sequence.Compared with the reported Chrysanthemum miR156,the 14th base was also different,and evolutionary analysis showed that the relationship was far.The mature sequence of miR156 was highly conserved,and the rest of its precursor sequence was quite different,especially the hairpin region.The expression of cmo-MIR156c was different in various organs of Chrysanthemum,with the highest expression in roots and tubular flowers.The heterologous expression of cmo-MIR156c in Arabidopsis changed the number of rosette leaves,the number of branches and flowering time,and affected the development of flowers and siliques.

References:

[1]OHMIYA A.Molecular mechanisms underlying the diverse array of petal colors in Chrysanthemum flowers[J].Breeding Science,2018,68(1):119-127.[2]XI L,WEN C,FANG S,et al.Impacts of strigolactone on shoot branching under phosphate starvation in Chrysanthemum (Dendranthema grandiflorum cv.Jinba)[J].Frontiers in Plant Science,2015(6):1-15.[3]WEI Q,MA C,XU Y J,et al.Control of Chrysanthemum flowering through integration with an aging pathway[J].Nature Communications,2017,8(1):1-11.[4]CHENG P,LIU Y,YANG Y,et al.[STBX]CmBES1[STBZ] is a regulator of boundary formation in Chrysanthemum ray florets[J].Horticulture Research,2020,7(1):1-12.[5]刘永平,杨静,杨明峰.植物开花调控途径[J].生物工程学报,2015,31(11):1553-1566.[6]CHO L H,YOON J,AN G.The control of flowering time by environmental factors[J].The Plant Journal,2017,90(4):708-719.[7]张翠桔,莫蓓莘,陈雪梅,等.植物miRNA作用方式的分子机制研究进展[J].生物技术通报,2020,36(7):1-14.[8]雷凯健,刘浩.植物调控枢纽miR156及其靶基因SPL家族研究进展[J].生命的化学,2016,36(1):13-20.[9]WANG J W,CZECH B,WEIGEL D.miR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana[J].Cell,2009,138(4):738-749.[10]KIM J Y,OH J E,NOH Y S,et al.Epigenetic control of juvenile-to-adult phase transition by the Arabidopsis SAGA-like complex[J].The Plant Journal,2015,83(3):537-545.[11]LITT A,IRISH V F.Duplication and diversification in the [STBX]APETALA1[STBZ]/FRUITFULL floral homeotic gene lineage:Implications for the evolution of floral development[J].Genetics,2003,165:821-833.[12]MATHIEU J,WARTHMANN N,KUTTNER F,et al.Export of FT protein from phloem companion cells is sufficient for floral induction in Arabidopsis[J].Current Biology,2007,17(12):1055-1060.[13]SCHWARZ S,GRANDE A V,BUJDOSO N,et al.The microRNA regulated SBP-box genes [STBX]SPL9 and SPL15[STBZ] control shoot maturation in Arabidopsis[J].Plant Molecular Biology,2008,67(1/2):183-195.[14]SHIKATA M,KOYAMA T,MITSUDA N,et al.Arabidopsis SBP-box genes [STBX]SPL10[STBZ],[STBX]SPL11 and SPL2[STBZ] control morphological change in association with shoot maturation in the reproductive phase[J].Plant and Cell Physiology,2009,50(12):2133-2145.[15]WANG S,WU K,YUAN Q,et al.Control of grain size,shape and quality by [STBX]OsSPL16[STBZ] in rice[J].Nature Genetics,2012,44(8):950-954.[16]STIEF A,ALTMANN S,HOFFMANN K,et al.Arabidopsis miR156 regulates tolerance to recurring environmental stress through SPL transcription factors[J].The Plant Cell,2014,26(4):1792-1807.[17]WANG L,ZHOU C M,MAI Y X,et al.A spatiotemporally regulated transcriptional complex underlies heteroblastic development of leaf hairs in Arabidopsis thaliana[J].The EMBO Journal,2019,38(8):1-12.[18]WANG Y M,LIU W W,WANG X W,et al.MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar[J].Horticulture Research,2020(7):1-12.[19]CHENG Y J,SHANG G D,XU Z G,et al.Cell division in the shoot apical meristem is a trigger for miR156 decline and vegetative phase transition in Arabidopsis[J].Proceedings of the National Academy of Sciences of the United States of America,2021,118(46):1-9.[20]陈熹.菊花花发育相关基因的挖掘与功能浅析[D].长沙:湖南农业大学,2022.[21]KUMAR S,STECHER G,LI M,et al.MEGA X:Molecular evolutionary genetics analysis across computing platforms[J].Molecular Biology and Evolution,2018,35(6):1547-1549.[22]CHEN C J,CHEN H,ZHANG Y,et al.TBtools:An integrative toolkit developed for interactive analyses of big biological data[J].Molecular Plant,2020,13(8):1194-1202.[23]秦梅,田心宇,于凯丽,等.miR156调控菊花逆境响应与开花的表达特性研究[J].西北植物学报,2023,43(3):374-381.[24]BETTI F,LADERA-CARMONA M J,WEITS D A,et al.Exogenous miRNAs induce post-transcriptional gene silencing in plants[J].Nature Plants,2021,7(10):1379-1388.[25]FU C X,SUNKAR R,ZHOU C,et al.Overexpression of miR156 in switchgrass (Panicum virgatum L.) results in various morphological alterations and leads to improved biomass production[J].Plant Biotechnology Journal,2012,10(4):443-452.[26]GAO R,GRUBER M Y,AMYOT L,et al.SPL13 regulates shoot branching and flowering time in Medicago sativa[J].Plant Molecular Biology,2018,96(1/2):119-133.[27]CAO D,LI Y,WANG J L,et al.GmmiR156b overexpression delays flowering time in soybean[J].Plant Molecular Biology,2015,89(4/5):353-363.[28]XU M L,HU T Q,ZHAO J F,et al.Developmental functions of miR156-regulated SQUAMOSA promoter-binding protein-like (SPL) genes in Arabidopsis thaliana[J].PLoS Genetics,2016,12(8):1-29.[29]ZHU Q H,HELLIWELL C A.Regulation of flowering time and floral patterning by miR172[J].Journal of Experimental Botany,2011,62(2):487-495.[30]ZHAO L,KIM Y,DINH T T,et al.miR172 regulates stem cell fate and defines the inner boundary of APETALA3 and PISTILLATA expression domain in Arabidopsis floral meristems[J].The Plant Journal,2007,51(5):840-849.[31]HUANG Z G,SHI T,ZHENG B L,et al.[STBX]APETALA2[STBZ] antagonizes the transcriptional activity of AGAMOUS in regulating floral stem cells in Arabidopsis thaliana[J].New Phytologist,2017,215(3):1197-1209.

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Last Update: 2023-11-30