|Table of Contents|

Establishment of Tissue Culture and Rapid Propagation Technology of Chrysanthemum morifolium ‘Niukou’ (Green)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年22
Page:
60-67
Research Field:
Publishing date:

Info

Title:
Establishment of Tissue Culture and Rapid Propagation Technology of Chrysanthemum morifolium ‘Niukou’ (Green)
Author(s):
LI Peiling1YANG Xincheng2YAO Jianjun2YIN Dongmei34
(1.School of Horticulture,Xinyang Agriculture and Forestry University,Xinyang,Henan 464000;2.Shanghai Honghua Horticulture Co.Ltd.,Shanghai 201605;3.School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei,Anhui 230036;4.School of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai 201418)
Keywords:
Chrysanthemum morifolium ‘Niukou’ (green)explantstissue culturerapid propagation
PACS:
S 682.1+1
DOI:
10.11937/bfyy.20231672
Abstract:
Chrysanthemum morifolium ‘Niukou’ (Green) were used as materials.The explants were treated by different sterilization methods,and the leaf adventitious bud induction,proliferation culture and rooting culture were carried out by adding different concentrations and different kinds of plant hormones,so as to screen the best explants,disinfection methods,and the best formula of induction,proliferation and rooting medium,in order to establish an efficient regeneration system of ‘Niukou’ (Green).The results showed that the optimum disinfection method was 0.1% HgCl2 for 9 minutes after 75% alcohol for 30 seconds,the contamination rate was 6.67% and the mortality rate was 0%.The most suitable culture-medium formulations for callus and adventitious buds induced by leaf discs were,MS+1.00 mg·L-1 6-BA+0.5 mg·L-1 NAA,under such circumstances,callus induction rate was 100%,and callus showed light green,loose and granular,while adventitic bud induction rate was 91.11%,and bud clusters were dense,well grown and dark green in color.The most suitable rooting medium was adding 0.3 mg·L-1 NAA to MS.

References:

[1]王碧玉.菊花再生及遗传转化体系的研究[D].沈阳:沈阳农业大学,2017.[2]程越.菊花脑再生体系建立的研究[D].南京:南京农业大学,2014.[3]周涌.菊花再生体系的建立及根癌农杆菌介导DAD1基因转化体系的优化[D].长沙:湖南农业大学,2007.[4]林青萍.菊花高效再生体系的建立及GAI基因转化菊花的研究[D].海口:华南热带农业大学,2006.[5]梁慧.菊花高频再生体系建立及根癌农杆菌介导遗传转化体系的优化[D].重庆:西南大学,2006.[6]孙磊,张启翔.利用薄层细胞培养技术建立‘铺地金’等菊花高效再生体系的研究[J].西北农业学报,2007,16(4):148-151.[7]全英杰,任镘蓉,杨雯婷,等.地被菊‘映洁红菲’再生及遗传转化体系的建立[J].北方园艺,2021(21):72-77.[8]吴志苹,高亦珂,范敏,等.菊花‘金不凋’再生及遗传转化体系的构建[J].分子植物育种,2020,18(1):150-158.[9]陈好,莫丽文,王泽峰,等.地被菊花‘珍粉’组织培养与植株再生体系的建立[J].分子植物育种,2023,21(5):1633-1640.[10]刘钰,周胜芳,夏豫川,等.蝴蝶兰外植体(花梗)消毒效果试验研究[J].西北园艺(综合),2023(1):48-51.[11]崔乐源,盛夏薇,郭佳琳,等.茶用菊花‘金丝皇菊’的组培快繁技术研究[J].天津农业科学,2023,29(1):7-11,26.[12]高宇琼,郭春喜,田鹏.红花木莲组织培养外植体消毒方法初步研究[J].安徽农学通报,2016,22(20):17-18,112.[13]闻永慧,陈双秀,汪琼,等.红纹凤仙花离体快繁技术体系的建立[J].北方园艺,2021(15):57-64.[14]王锴乐,纪宝玉,裴莉昕,等.怀菊快繁体系及组培苗分级标准的建立[J].北方园艺,2021(24):122-128.[15]勾畅,武慧,将达,等.激素配比对不同基因型菊花再生体系的调控[J].北方园艺,2013(13):135-137.[16]路国辉,吴丹,刘盼盼,等.海南三七的组织培养和快速繁殖[J].北方园艺,2022(12):99-105.[17]李雪艳,胡新颖,王伟东,等.无病毒百合‘Sorbonne’试管内小鳞茎直接再生体系建立[J].北方园艺,2021(9):80-86.[18]刘秀杰,田騄頔,裴毅,等.桔梗的组培离体再生[J].北方园艺,2017(17):40-43.[19]桂晴,郝明灼,邹义萍,等.冬青属植物组培快繁研究进展[J].北方园艺,2022(24):115-122.[20]孟军,韩杰,祖恩普.牡丹组织培养研究进展[J].北方园艺,2007(1):153-154.[21]张家瑛.贡菊离体快繁体系优化及种苗分级标准研究[D].广州:广州中医药大学,2016.[22]曲晓慧.几种菊属野生植物再生体系的建立[D].南京:南京农业大学,2020.[23]李辛雷,陈发棣,王红,等.菊花外植体再生体系的研究[J].上海农业学报,2004,20(2):13-16.[24]肖政,范崇辉,金万梅.生长调节物质对菊花‘小金黄’叶片再生不定芽的影响[J].西北林学院学报,2016,24(6):50-53.[25]熊威.四个菊花品种再生体系的研究及CDS基因的遗传转化[D].武汉:华中农业大学,2010.[26]孙利忠,陈敬,胡秀锦,等.红叶石楠离体组培快繁技术体系研究[J].北方园艺,2022(14):49-56.[27]董凤丽.甘菊NAC基因的功能研究及对露地菊花的遗传转化[D].哈尔滨:东北林业大学,2014.[28]周洲,李永丽,姜玲,等.菊花‘绿鹦哥’的组织培养和快速繁殖[J].北方园艺,2009(10):110-112.[29]龚建国,杨雪,李婷,等.利用叶片再生进行滁菊组培扩繁[J].安徽农业科学,2013,41(18):7788-7789.

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Last Update: 2023-12-15