|Table of Contents|

Amplification and Sequence Analysis of rbcL Gene in Hippophae rhamnoides

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年21
Page:
42-52
Research Field:
Publishing date:

Info

Title:
Amplification and Sequence Analysis of rbcL Gene in Hippophae rhamnoides
Author(s):
LIU Qingqing1; 2; YANG Kaiyu3; MA Yuhua1; SHI Hui1; YE Guisheng1; FENG Zhipeng1
(1.College of Agriculture and Animal Husbandry，Qinghai University，Xining，Qinghai 810016;2.Academy of Animal Husbandry and Veterinary Sciences，Qinghai University，Xining,Qinghai 810016；3.Chengdu Nature Reserve and Wildlife Conservation Center，Chengdu，Sichuan 610031)
Keywords:
Hippophae rhamnoides subsp.sinensis; rbcL; sequence analysis; variation; systematic classification
PACS:
S 793.6
DOI:
10.11937/bfyy.20231588
Abstract:
Taking Hippophae rhamnoides from 11 regions in Qinghai Province and Tibetan sea buckthorn and ribbed sea buckthorn from Qilian county as test materials，a genetic distance matrix and phylogenetic tree were constructed using chloroplast gene marker rbcL to study the genetic variation of Hippophae rhamnoides in different regions of Qinghai Province，in order to provide reference for the classification of sea buckthorn plants.The results showed that the length of the rbcL region of all three species of sea buckthorn plants was 1 501 bp，and there was no length variation.There were different variation sites in the rbcL gene sequences of three species of sea buckthorn plants，and there were also a small number of variation sites between the rbcL genes of Hippophae rhamnoides in different regions.Cluster analysis with some rbcL gene sequences on NCBI revealed basic consistency between morphological and molecular methods for systematic classification of sea buckthorn.This study provided new ideas and methods for future research on the phylogenetic evolution of sea buckthorn using rbcL sequences，laying the foundation for the development and utilization of sea buckthorn.

References:

［1］南吉斌,杨广环,吴天彧,等.西藏3种沙棘属植物抗旱性比较研究［J］.西北农林科技大学学报(自然科学版),2021,49(1):37-47.［2］WANG H F,LIU H，YANG M B,et al.Phylogeographic study of Chinese seabuckthorn (Hippophae rhamnoides subsp.sinensis Rousi) reveals two distinct haplotype groups and multiple microrefugia on the Qinghai-Tibet Plateau［J］.Ecology and Evolution,2014,4(22):4370-4379.［3］王汉,乔枫.沙棘属逆境生理研究进展［J］.北方园艺,2021(9):138-142.［4］YAO B N,LIAO F Y,YANG J Y,et al.Effect of sea buckthorn extract on production performance，serum biochemical indexes，egg quality，and cholesterol deposition of laying ducks［J］.Frontiers in Veterinary Science,2023,10:1127117.［5］WANG Z,ZHAO F L,WEI P P，et al.Phytochemistry，health benefits，and food applications of sea buckthorn (Hippophae rhamnoides L.):A comprehensive review［J］.Frontiers in Nutrition,2022,9:1036295.［6］马玉花,冶贵生,向前胜,等.基于ITS 序列探讨沙棘属植物的系统发育关系［J］.应用生态学报,2014,25(10):2985-2990.［7］张吉科,张小民,张国伟.中国沙棘表皮毛的形态分布和类群研究［J］.林业科学，1995(5):408-413,481-482.［8］庞海龙,苏雪,张辉,等.沙棘属植物种子微形态特征研究［J］.植物科学学报,2012，30(1):8-14.［9］潘志辉.沙棘叶片形态特征的数学分析［J］.安徽农业科学,2013,41(12):5408-5409.［10］颜新林,管中荣,温雯,等.基于SSR标记的芥菜品种鉴定技术体系建立及应用［J］.植物遗传资源学报,2021,22(3):758-770.［11］杨永虎.沙棘的鉴别和亚种分类［J］.养殖技术顾问,2014(5):91.［12］李蓉,于永涛.DNA分子标记及在沙棘遗传育种中的应用展望［J］.国际沙棘研究与开发,2008(2):19-25.［13］张秀荣,都桂芳,马占青.沙棘雌雄株间同功酶差异初探［J］.内蒙古农业科技,1988(2):34,31.［14］王柏青,王耀辉.中国沙棘的染色体核型分析［J］.北华大学学报(自然科学版)，2000(5):407-409.［15］王彬.中国沙棘染色体核型及进化研究［J］.湖北农业科学,2011,50(8):1599-1601.［16］RUAN C J，QIN P，ZHENG J W，et al.Genetic relationships among some cultivars of sea buchthorn from China,Russia and Mongolia based on RAPD analysis［J］.Scientia Horticulturae,2004,101:417-426.［17］TIAN C J，LEI Y D，SHI S H，et al.Genetic diversity of sea buckthorn populations in northeastern and northwestern China as revealed by ISSR markers［J］.New Forests，2004,27:229-237.［18］廖祥儒,朱新产,万怡震.核酸分析技术与植物的分子分类［J］.生物技术通报，1995（6）:3-5.［19］姜芳燕,杨宁,菅盼盼,等.基于rbcL和tufA基因的石莼和浒苔系统发育分析［J］.基因组学与应用生物学,2018,37(7):3012-3018.［20］PRASHANT S,ARSHIA F,ARUN K M.Molecular phylogeny and evogenomics of heterocystous cyanobacteria using rbcL gene sequence data［J］.Annals of Microbiology,2015,65(2):799-807.［21］范豫杰,黄磊,苏世达,等.基于matK+rbcL+trnH-psbA联合分析法对罂粟属的植物鉴定［J］.昆明医科大学学报,2023,44(1):12-18.［22］张晓芹,毛佳乐,王慧玉,等.基于rbcL序列对猫人参及其近缘种的分子鉴定研究［J］.山东中医药大学学报,2022,46(6):758-765.［23］冯洁.栽培丹参居群多重分子标记遗传多样性联合分析［D］.重庆：西南大学,2022.［24］TURK D T,VASCOTTO I,FRANC J,et al.Evaluation of the rbcL marker for metabarcoding of marine diatoms and inference of population structure of selected genera［J］.Frontiers in Microbiology,2023,14:1071379-1071379.［25］巩超彦,南芳茹,冯佳,等.串珠藻目植物rbcL基因的适应性进化分析［J］.海洋与湖沼,2017,48(3):527-535.［26］侯哲,娄晓鸣,李昂,等.11种唐松草属(Thalictrum)rbcL基因的密码子偏好性研究［J］.江苏农业科学,2023,51(3):46-53.［27］刘小平,郭崇炎,周蓉,等.锐尖山香圆基因组DNA提取方法比较研究［J］.南方林业科学,2022,50(3):5-9.［28］陈学林,马瑞君,孙坤,等.中国沙棘属种质资源及其生境类型的研究［J］.西北植物学报,2003(3):451-455.［29］马利.沙棘组培再生体系建立及其〖STBX〗HrFAD2〖STBZ〗基因的克隆与表达分析［D］.呼和浩特：内蒙古农业大学,2022.［30］廉永善,陈学林.沙棘属植物的系统分类［J］.沙棘,1996，9（1）:10.［31］陈金金,赵明霞,姜树,等.基于rbcL-a和ITS的枸杞鉴别|、遗传关系分析及ITS假基因的发现［J］.生物技术通报,2017,33(5):123-130.［32］张志翔,张勇,高宗庆.沙棘属和胡颓子属叶表皮形态与分类的扫描电镜研究：Ⅱ.沙棘属叶表皮形态与分类［J］.植物研究,1992(4):407-415.［33］王林,李海波,陈汉鑫,等.海拔高度对中国沙棘水碳代谢和生长的影响：以关帝山区为例［J］.生态学报,2023,43(5):1995-2004.［34］陈国娟.卧龙自然保护区不同海拔的中国沙棘(Hippophae rhamnoides subsp.sinensis)天然群体的遗传多样性分析［D］.成都：中国科学院成都生物研究所，2007.［35］刘光琇.沙棘(Hippophae L.)属植物的稳定碳同位素特征及遗传多样性研究［D］.兰州:兰州大学,2004.［36］陈纹.沙棘属几个中国特有类群的遗传多样性研究［D］.兰州:西北师范大学,2004.［37］吴琼.中国沙棘、云南沙棘表型多样性研究［D］.兰州：西北师范大学,2007.［38］李明.中国北方普通杏和西伯利亚杏遗传多样性研究［D］.杨凌：西北农林科技大学,2014.［39］李霓,蒋严妃,苏雪,等.肋果沙棘北缘居群的遗传多样性与遗传结构［J］.广西植物，2016,36(5):557-563.

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Last Update: 2023-12-05