|Table of Contents|

Analysis on Bacterial Diversity of Alfalfa Rhizosphere Soil in Saline-alkali Land

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年23
Page:
75-82
Research Field:
Publishing date:

Info

Title:
Analysis on Bacterial Diversity of Alfalfa Rhizosphere Soil in Saline-alkali Land
Author(s):
WANG Xiaochun; YANG Weidi; GAO Ting
(Animal Sciences Institute，Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750000)
Keywords:
saline-alkali land; alfalfa; rhizosphere soli; bacterial community; high-throughput sequencing
PACS:
S 963.22+3.3
DOI:
10.11937/bfyy.20231313
Abstract:
Take the rhizosphere soil of alfalfa as research object，the rhizosphere and non-rhizosphere (control) soil were collected，and high-throughput sequencing technology of 16S rRNA was used to study the rhizosphere bacterial community characteristics of alfalfa in severe saline-alkali land of Ningxia′ north，in order to provide reference for improving the saline-alkali land by planting alfalfa and using microbial resources of saline-alkali land.The results showed that,soil enzymes activities and microbial diversity in the rhizosphere of alfalfa planted in saline-alkali land.The pH of the sampling area was 8.57，and the salt concentration was 669.51 mg·L-1，which belonged to the middle and severe saline-alkali land.The activities of phosphatase,sucrase and urease in alfalfa rhizosphere soil were significantly higher than those in non-rhizosphere soil.A total of 10 488 OTUs were obtained by high-throughput sequencing,distributed in 94 phyla，191 classes，391 orders,549 families，910 genus and 448 species.The dominant phylum were Proteobacteria,unidentified_Bacteria，Bacteroidota,Acidobacteria and Gemmatimonadetes.The dominant genus were Halomonas，Pseudomonas，unidentified_Actinomarinales and Azoarcus.The relative abundance of dominant bacterial genera Halomonas,Pseudomonas,and Azoarcus was significantly higher in rhizosphere soil than in non rhizosphere soil.

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Last Update: 2023-12-20