|Table of Contents|

Effects of Different Cultivation Formulas on Growth and Extracellular Enzyme Activity of Cultured Agrocybe cylindracea

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年19
Page:
114-121
Research Field:
Publishing date:

Info

Title:
Effects of Different Cultivation Formulas on Growth and Extracellular Enzyme Activity of Cultured Agrocybe cylindracea
Author(s):
ZHOU Yu1LIU Penghu2LIU Bo1SU Rongrong1ZHOU Jiajia1
(1.Southwest Guizhou Institute of Agricultural and Forestry Sciences,Xingyi,Guizhou 562400.2.National Engineering Research Center of Juncao Technology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)
Keywords:
Agrocybe cylindraceaMiscanthus floridulusextracellular enzyme activity
PACS:
S 646
DOI:
10.11937/bfyy.20231181
Abstract:
Agrocybe cylindrace were cultivated with Miscanthus floridulus and cottonseed shells as the main raw materials,and design of cultivation formulations according to different addition ratios,the effects of five clinker cultivation formulations on mycelium growth,biological efficiency,agronomic traits and five extracellular enzyme activities of A.cylindracea were studied,in order to provide reference for improving the comprehensive utilization value of mushroom resources such as Miscanthus floridulus and promoting the sustainable development of China′s mushroom industry.The results showed that formulation P3 had the best performance on mycelial growth,agronomic characteristics,first crop yield and biological efficiency of A.cylindracea,these traits were superior to other formulations,with neat and dense mycelial growth,the shortest germination time,the best morphological characteristics of fruiting entities,the first tide mushroom yield of (317.187±1.980) g per bag,and the biological efficiency of 63.44%.The activity changed of extracellular enzymes such as CMCase,xylanase,amylase,laccase and proteinase in the whole growth period of A.cylindracea under different cultivation formulas had obvious stages,and the trend of activity of the same enzyme in different cultivar formulations was generally consistent.The correlation analysis between mycelial growth rate and five extracellular enzymes showed that CMCase,xylanase and amylase were positively correlated with mycelial growth rate,among which xylanase and amylase were highly significantly positively correlated,indicating that the cultivation formula with fast mycelial growth rate could promote the secretion of mycelial extracellular enzymes,and in order to guarantee the supply of nutrients,the mycelium secreted more CMCase,xylanase and amylase to degrade the cultivated material.Comprehensive analysis,the P3 formulation was most suitable for the growth of tea tree mushrooms.

References:

[1]邓果特,刘清波,蒋建雄,等.五节芒基因组大小测定[J].植物遗传资源学报,2013,14(2):339-341.[2]萧运峰,王锐,高洁.五节芒生态:生物学特性的研究[J].四川草原,1995(1):25-29.[3]陈慧娟,宁祖林,张卓文.五节芒生物学特性及能量生产动态变化[J].草业学报,2012,21(6):252-257.[4]周晶,林兴生,林辉,等.菌草研究与应用进展[J].福建农林大学学报(自然科学版),2020,49(2):145-152.[5]曹秀明,蔡杨星,林占熺.5种菌草草粉栽培灰树花培养基配方筛选[J].福建农业科学,2019(1):14-19.[6]胡应平,林兴生,赖由运,等.菌草混合莲子废弃物栽培平菇效果评价[J].南方农业学报,2019,50(7):1557-1564.[7]杨丽秋,范锦琳,刘欣怡,等.菌草对灵芝生长状况及营养成分的影响[J].福建农业科学,2017,32(5):508-511.[8]薛志香,曾峰,曹秀明,等.鲜菌草与干菌草灭菌前后碳氮含量及碳氮比[J].北方园艺,2018(1):150-154.[9]聂国添,黄千慧,苗娟,等.五节芒栽培糙皮侧耳的子实体质构分析[J].食用菌学报,2016,23(1):31-36.[10]戴玉成,周丽伟,杨祝良,等.中国食用菌名录[J].菌物学报,2010,29(1):1-21.[11]王谦,黄媛媛,宋长林.常压室温等离子体(ARTP)诱变技术选育柱状田头菇(茶树菇)优良菌株研究初探[J].菌物学报,2017,36(6):776-783.[12]王龙,周庆平,石国华,等.茶树菇Agrocybe cylindracea及近缘种种质资源研究现状[J].北方园艺,2021(15):134-142.[13]包辰.茶树菇多酚提取及其功能学特性的研究[D].福州:福建农林大学,2013.[14]巫伟鹏,黄碧霞,关鑫,等.茶树菇凝集素检测醋酸格拉替雷诱导小鼠的CD8+T淋巴细胞表面糖基化研究[J].现代食品科技,2016,32(5):8-13.[15]陈子涵,刘金娟.六种食用菌体外抗氧化及抗细胞增殖活性研究[J].生物技术通报,2019,35(11):104-108.[16]ZHAO H,LI J,ZHANG J,et al.Purification,in vitro antioxidant and in vivo anti-aging activities of exopolysaccharides by Agrocybe cylindracea[J].International Journal of Biological Macromolecules,2017,102:351-357.[17]CHIEN R C,TSAI S Y,LAI E Y C,et al.Antiproliferative activities of hot water extracts from culinary-medicinal mushrooms,Ganoderma tsugae and Agrocybe cylindracea (Higher Basidiomycetes) on cancer cells[J].International Journal of Medicinal Mushrooms,2015,17(5):453-462.[18]梁一,刘洪洪,关鑫,等.茶树菇Agrocybe aegerita新泛素偶联酶蛋白表达及抗肿瘤活性研究[J].现代食品科技,2015,31(12):1-5.[19]李爱臣,房磊.柱状田头菇硒提取物的提取工艺和抗疲劳效果[J].食用菌学报,2015,22(4):75-80.[20]吴圣进,王灿琴,汪茜,等.草菇生长发育过程中胞外酶活性变化[J].中国食用菌,2015,34(1):53-56.[21]曾春函,王传华.我国主要蜜环菌生物种胞外酶活性对温度的种特异性响应[J].菌物学报,2019,38(2):195-209.[22]曹永佳,马鸿飞,崔宝凯,等.不同固体发酵培养基下三种白腐真菌分泌的木质纤维素酶活性[J].菌物学报,2021,40(5):1123-1139.[23]王红,刘岩岩,李红,等.菌包后熟期高温胁迫对黑木耳生理特性及生长发育的影响[J].食用菌学报,2022,29(5):33-42.[24]陆欢,王小艳,尚晓冬,等.不同碳氮源对花脸香蘑胞外酶活性的影响[J].菌物学报,2021,40(12):3286-3303.[25]刘晓敏,陈向东,张薇薇,等.不同蜜环菌菌株的鉴定及胞外酶活性研究[J].中国农学通报,2020,36(24):99-106.[26]张职视.斑玉蕈菌包成熟度判定多尺度指标的建立与应用[D].福州:福建农林大学,2016.[27]吴雪君,安琪,戴玉成.糙皮侧耳(平菇)菌株CCEF89的生理生化研究[J].菌物学报,2015,34(4):621-631.[28]马运龙,林占熺,陈超前,等.不同栽培基质配方对银耳胞外酶活性的影响[J].福建农业科技,2021,52(11):23-27.[29]孙淑静,单书凯,李钊锋,等.不同微量元素对斑玉蕈菌丝生长及酶活的影响[J].福建农林大学学报(自然科学版),2015,44(6):639-645.[30]李治平,陈明杰,王耀冉,等.草菇子实体蛋白酶的分离纯化及其酶学性质[J].食用菌学报,2021,28(6):24-30.[31]林辉,李娜娜,李佳欢,等.斑玉蕈出菇产量与胞内外碳代谢的相关性分析[J].菌物学报,2022,41(9):1506-1518.[32]丁明辉.五节芒栽培茶薪菇试验[J].食用菌,2009,31(1):24.[33]吴亚召,张文隽,雷萍,等.茶树菇生长发育过程中几种胞外酶活性的变化[J].西北农林科技大学学报(自然科学版),2018,46(11):115-120.[34]冯作山,邹亚杰,胡清秀.白灵侧耳栽培过程中胞外酶和呼吸酶活及离子流速的研究[J].菌物学报,2014,33(2):341-354.[35]雷萍,吴亚召,张文隽,等.榆黄蘑不同生长发育期胞外酶活性变化研究[J].中国食用菌,2020,39(11):57-61.[36]程泳春,李佳欢,苏财自,等.温度对斑玉蕈后熟阶段生理活性影响[J].中国食用菌,2020,39(4):26-31.

Memo

Memo:
-
Last Update: 2023-11-29