|Table of Contents|

Study on in vitro Rapid Propagation of Elsholtzia bodinieri

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年23
Page:
60-67
Research Field:
Publishing date:

Info

Title:
Study on in vitro Rapid Propagation of Elsholtzia bodinieri
Author(s):
ZHAO Jie; HOU Yuqi; ZHENG Wei; XU Xiaodan
(College of Architecture and City Planning，Kunming University of Science and Technology，Kunming，Yunnan 650500)
Keywords:
Elsholtzia bodinieri; tissue culture;  in vitro rapid propagation
PACS:
S 681.9
DOI:
10.11937/bfyy.20231147
Abstract:
The stem tips of Elsholtzia bodinieri were used as explants and different concentrations of plant growth regulators were used in tissue culture experiments.The effects of different sterilization treatments,media types，hormone concentrations and transplanting substrates on the initial culture，subculture proliferation，rooting culture and seedling transplanting of Elsholtzia bodinieri were studied.In order to lay a foundation for in vitro propagation，in vitro mutagenesis and genetic transformation of Elsholtzia bodinieri.The results showed that the optimum sterilization method for the stem tips of Elsholtzia bodinieri was 75% alcohol for 30 seconds＋0.1% HgCl2 for 6 minutes，and the survival rate was 76.67%.The optimum primary culture medium was MS＋6-BA 1.0 mg·L-1＋IAA 0.10 mg·L-1，and the induction rate was up to 88.89%.The optimum proliferation medium was MS＋6-BA 0.3 mg·L-1＋NAA 0.05 mg·L-1，the proliferation coefficient was 12.91.The best rooting medium was 1/2MS＋IBA 1.00 mg·L-1，with a rooting rate of 97.78%.After 4 days of open cover，the seedlings were transplanted into a mixed substrate of 1 humus soil∶1 perlite∶1 peat，with a survival rate of 91.11%.

References:

［1］中国科学院中国植物志编辑委员会.中国植物志-第六十六卷［M］.北京：科学出版社，1977.［2］邓芸，盖琼辉.紫苏·香紫苏·东紫苏的比较研究［J］.北方园艺，2010(4)：218-220.［3］王春梅，龙平，许利嘉，等.凤尾茶的来源及近代研究［J］.中国现代中药，2013，15(3):250-255.［4］赵明霞.东紫苏的药用成分及功能分析［J］.中国民族民间医药，2009，18(21)：15.［5］唐仁华，杨礼攀，张红霞，等.云南省15个居群的东紫苏叶挥发油化学型研究［J］.中药材，2020，43(4)：886-890.［6］YANG L，DU J，LI R，et al.Bodiniosides S-Y，seven new triterpenoid saponins from Elsholtzia bodinieri and their anti-influenza activities［J］.Molecules，2021，26(21)：6535.［7］昆明市卫生局.昆明民间常用草药［M］.昆明:昆明市卫生局，1970.［8］温敏,吴润芳,李吉涛.东紫苏挥发油成分及其应用研究进展［J］.中国中医药现代远程教育,2020,18(23):149-151.［9］张丽梅，王桥，龙华，等.滇东东紫苏资源的调查与评价［J］.黑龙江农业科学，2013(4)：83-85.［10］桂晴,郝明灼,邹义萍,等.冬青属植物组培快繁研究进展［J］.北方园艺,2022(24):115-122.［11］姚永宏，周正科，侯渝嘉，等.福鼎大白茶外植体选择及愈伤组织诱导条件初探［J］.现代农业科学，2009，16(4)：37-38,84.［12］吴斌.山茶‘耐冬’愈伤组织诱导与植株再生的研究［D］.成都：四川师范大学，2013.［13］孙利忠,陈敬,胡秀锦,等.红叶石楠离体组培快繁技术体系研究［J］.北方园艺,2022(14):49-56.

Memo

Memo:
-
Last Update: 2023-12-20