|Table of Contents|

Application Progress of KASP Marker Technology on Crop Gene Location

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年19
Page:
122-127
Research Field:
Publishing date:

Info

Title:
Application Progress of KASP Marker Technology on Crop Gene Location
Author(s):
ZHAO Yue1; SUN Yufeng1; XU Lei2; WANG Pan1; BIAN Jing1; WANG Xiaonan1
(1.Daqing Branch,Heilongjiang Academy of Sciences，Daqing，Heilongjiang 163319;2.Daqing Branch,Heilongjiang Academy of Agricultural Sciences，Daqing，Heilongjiang 163319)
Keywords:
single nucleotide polymorphism (SNP); kompetitive allele specific PCR (KASP); crop traits; genetic map; gene location
PACS:
S 188
DOI:
10.11937/bfyy.20231080
Abstract:
Based on next generation sequencing (NGS)，single nucleotide polymorphism (SNP) of the third-generation molecular marker was developed.Mutations of a single nucleotide cause polymorphism in nucleic acid sequences.It has the characteristics of good genetic stability，high density and secondary genotype，and is easy to realize high throughput and automatic detection.Kompetitive allele specific PCR (KASP) is a genotyping technology which is a gel-free fluorescent polymerase chain reaction based on SNP and has been widely used in genetic breeding of animals and plants.In this study，the research progresses of KASP technology in fine mapping of genes related to important traits such as crop yield，quality and resistance were reviewed from experimental population selection and specific application strategies in crop gene location.And its advantages and disadvantages were summarized.Suggestions such as crop KASP typing technology system optimization and KASP database of functional gene polymorphic loci established were put forward to further promote its application,in order to provide reference for the genetic regulation of crop traits and accelerate the transformation from traditional experience breeding to precision breeding.

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Last Update: 2023-11-29