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¡¶±±·½Ô°ÒÕ¡·[ISSN:1001-0009/CN:23-1247/S]

Issue:

2023Äê18

Page:

62-69

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Title:

Genetic Relationship Analysis of Lespedeza Based on ISSR Molecular Markers

Author(s):

LI Jinqi; ZHOU Xiaohui; WEI Jie; YAO Ruihong; JIN Mengran; SHI Baosheng

(College of Landscape Architecture and Tourism£¬Hebei Agricultural University£¬Baoding,Hebei 071000)

Keywords:

Lespedeza; ISSR; genetic relationships; genetic diversity

PACS:

S 685.99

DOI:

10.11937/bfyy.20230605

Abstract:

The genetic diversity and genetic relationship of 29 plant materials of Lespedeza were studied by inter simple sequence repeat(ISSR) molecular marker technology£¬and fingerprints were constructed£¬in order to provide reference for the introduction and breeding of Lespedeza in North China.The results showed that a total of 10 polymorphic ISSR primers were screened£¬PCR amplification was performed by polymorphic ISSR primers£¬and a total of 93 bands were amplified£¬included 92 polymorphic bands£¬and the polymorphism ratio (PPB) was 98.92%£¬and primer UBC-812 was selected to construct a fingerprint.The average effective gene number was 1.477 5 and the average Nei¡äs genetic diversity index (H) was 0.293 3.Through UPGMA cluster analysis£¬when the genetic similarity coefficient was 0.61£¬the 29 materials could be divided into 3 groups£¬which was not completely consistent with the traditional classification.In conclusion,the genetic diversity of Lespedeza was high£¬and the selected varieties of Lespedeza bicolor were not completely clustered£¬indicated that the genetic diversity of Lespedeza bicolor cultivated by cross breeding and other methods had been significantly improved.

References:

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Last Update: 2023-11-23