|Table of Contents|

Comparison of Methods for Identification of Resistance to Pear Fire Blight and Screening of Resistant Rootstock Germplasm

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年19
Page:
29-35
Research Field:
Publishing date:

Info

Title:
Comparison of Methods for Identification of Resistance to Pear Fire Blight and Screening of Resistant Rootstock Germplasm
Author(s):
JIANG YuanWEI JieWANG YanCUI LongXIE HongjiangLI Yongfeng
(Agricultural Scientific Institute of 2nd Division of Xinjiang Production and Construction Corps,Tiemenguan,Xinjiang 841005)
Keywords:
fire blightidentification methodsinoculation concentrationresistant rootstockscreening
PACS:
S 661.2
DOI:
10.11937/bfyy.20230493
Abstract:
Taking container seedling of Pyrus betulaefolia Bunge as test material,three kinds of artificial inoculation methods were used,namely dipping method,needle inoculation method and spray method,to investigate the time of incidence,incidence rate and disease index,compare the inoculation effect of Pyrus betulaefolia Bunge under different inoculation methods,screen the suitable method of pear fire blight inoculation and optimize the inoculum concentration for the best inoculation method,in order to screen the accurate and rapid identification method of pear fire blight and understand the resistance level of different rootstock germplasm to pear fire blight.The results showed that the incidence rate and disease index of the three inoculation methods were as follows,seedling dipping method>seedling spraying method>seedling needling method,and the shortest days for investigating the susceptibility and resistance were 7-9 days,12-33 days and 33-38 days.The seedling dipping method had the fastest incidence rate,good uniformity and easy operation,which could effectively shorten the inoculation time and improve the inoculation efficiency.When the OD600 value of the bacterial solution was 1.2,the incidence rate and disease index were stable over time with relatively small fluctuations,which could be used as a suitable inoculation concentration for the seedling dipping method.On this basis,five collections of Pyrus betulaefolia Bunge germplasm from different sources were identified by inoculation against fire blight at the seedling stage.The results showed that all the five germplasm were susceptible or highly susceptible to the disease,and the Pyrus betulaefolia Bunge rootstock resources of diseaseresistant need to be further explored.

References:

[1]李洪涛,张静文,盛强,等.我国20个梨品种(种质)对国外梨火疫病菌的抗病性评价[J].果树学报,2019,36(5):629-637.[2]李晓妹,韩丽丽,何亚南,等.20个苹果品种(类型)对梨火疫病菌的抗病性评价[J].植物检疫,2022,36(4):6-12.[3]王俊,高建诚,巴音克西克,等.利用电加热自动消毒修枝剪阻断梨火疫病田间传播[J].植物检疫,2022,36(2):25-28.[4]陈励坤,徐叶挺,王永鹏,等.新疆梨种质资源的火疫病抗性评价[J].中国果树,2022(8):16-22.[5]刘华威,王晓鸣,郭庆元,等.梨种质对梨火疫病的抗性研究[J].植物遗传资源学报,2008,9(2):195-200.[6]段红雁,李紫英,王兰,等.5种蔷薇科果树对梨火疫病的抗性[J].中国果树,2022(2):65-67,74.[7]张乐,石秀丽.用离体巴梨枝条测定梨火疫病菌的致病性[J].植物检疫,1993,7(2):104-105.[8]鲁晏宏,郝金辉,罗明,等.梨火疫病拮抗菌筛选及温室防效测定[J].微生物学通报,2021,48(10):3690-3699.[9]徐琳赟,古丽孜热·曼合木提,韩剑,等.香梨内生拮抗细菌的筛选及对梨火疫病的生防潜力[J].西北植物学报,2021,41(1):132-141.[10]曹玉芬,刘凤之,胡红菊,等.梨种质资源描述规范和数据标准[M].北京:中国农业出版社,2006.[11]继佐军,葛惠佳,许向阳.番茄芝麻斑病苗期抗性鉴定方法及抗病种质资源筛选[J].东北农业大学学报,2009,40(11):23-27.[12]姜翠翠,曾少敏,陈小明,等.110份梨种质资源对主要叶部病害的田间抗病性评价[J].中国南方树,2022,51(5):170-177,181.[13]朱凌丽,徐建,姚协丰,等.厚皮甜瓜种质蔓枯病抗性评价与遗传多样性分析[J].江苏农业学报,2021,37(2):454-464.[14]索欢,陈龙正,徐海,等.小白菜根肿病接种鉴定研究[J].江苏农业科学,2016,44(5):193-195.[15]王铭,臧丽丽,范凯,等.黄萎病菌毒素联合法鉴定棉花对黄萎病的抗性[J].中国农业科学,2015,48(9):1678-1688.[16]杨书青,闫宁宁,胡栓红,等.辣椒抗疫病性鉴定及辣椒疫霉药剂敏感性分析[J].北方园艺,2022(1):53-58.[17]郑坤,姜景彬,康立功,等.番茄白粉病苗期抗病性鉴定方法及抗病种质资源筛选[J].植物保护,2012,38(5):105-107.[18]何永宏,曾乙心,刘林,等.温度和品种抗性对烟草青枯病潜育期的影响[J].烟草科技,2017,50(6):16-20,32.[19]王登明,张学军,冯炯鑫,等.甜瓜枯萎病接种方法比较试验初报[J].中国瓜菜,2009(5):38-39.[20]雷晶.番茄苗期多抗性鉴定方法研究及抗病种质资源筛选[D].哈尔滨:东北农业大学,2007.

Memo

Memo:
-
Last Update: 2023-11-28