|Table of Contents|

Novel Antimicrobial Peptide on Control of Tomato Gray Mold and Its Mechanism

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年18
Page:
18-27
Research Field:
Publishing date:

Info

Title:
Novel Antimicrobial Peptide on Control of Tomato Gray Mold and Its Mechanism
Author(s):
JIA Yanli; HAN Ziwei; QIU Yan
(College of Food Science and Biology,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018)
Keywords:
antimicrobial peptides; tomato gray mold; control effect; antifungal mechanism; antioxidation
PACS:
S 641.2
DOI:
10.11937/bfyy.20230407
Abstract:
Tomato,antimicrobial peptides (AMPs) and tomato Botrytis cinerea were used as test materials.The antifungal effects of CB and D20I-39 on tomato B.cinerea and the relative mechanisms were investigated by plate experiments.The control effects of AMPs on tomato leaves infected by gray mold and the indexes of disease resistance and antioxidation were analyzed by pot experiments,in order to provide reference for AMPs to control tomato gray mold.The results showed that both CB and D20I-39 could inhibit mycelial growth and spore germination of B.cinerea dosedependently and caused leakage of intracellular nucleic acid and protein in B.cinerea.After treatment of CB and D20I-39,the mycelia of B.cinerea were flattened,shrunken and narrowed.And as the concentration increased,the mycelial folds became more severe and even rupture occurred.In pot experiments,the preventive test demonstrated the control effects of CB and D20I-39 were 96.37%±0.13% and 83.41%±0.17%,respectively and the therapeutic test exhibited the control effects of CB and D20I-39 were 14.86%±0.35% and 59.74±0.23%,respectively.AMPs could increase the activities of PAL,SOD and the levels of Pro,whereas decreased MDA content.The results indicated that the novel AMPs CB and D20I-39 inhibited B.cinerea by destorying cell membrane to exert the antifungal activity,and reduced the incidence and diseases index of tomato gray mold.CB and D20I-39 had good control effects on tomato gray mold.

References:

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Last Update: 2023-11-22