|Table of Contents|

Optimization of Deproteinization Process and Biological Activity of Adiantum capillus-veneris Polysaccharide by Response Surface Methodology

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年20
Page:
77-85
Research Field:
Publishing date:

Info

Title:
Optimization of Deproteinization Process and Biological Activity of Adiantum capillus-veneris Polysaccharide by Response Surface Methodology
Author(s):
YANG Lijun; LUO Wei; CUI Chenxu; GAO Han; HE Dingxuan; DONG Cui
(College of Pharmaceutical Engineering，Xinyang Agriculture and Forestry University，Xinyang，Henan 464000)
Keywords:
Adiantum capillus-veneris; polysaccharide; Sevage method; lowering blood sugar; antioxidant
PACS:
TS 241
DOI:
10.11937/bfyy.20230239
Abstract:
Taking Adiantum capillus-veneris as test material,the optimization method of combining single factor experiments and response interviews was used to optimize the deproteinization process of polysaccharides from Adiantum capillus-veneris.The effects of polysaccharides from Adiantum capillus-veneris before and after deproteinization were measured α-glucasidase inhibitory activity of glucosidase and its ability to scavenge DPPH free radicals were expected,in order to provide reference for the comprehensive utilization of polysaccharides from Adiantum capillus-veneris.The results showed that the optimal deproteinization process conditions of Adiantum capillus-veneris polysaccharides were as follows,the volume ratio between polysaccharide solution and Sevage reagent was 2∶1，the deproteinization time was 10 minutes，the deproteinization time was 5 times，the protein removal rate could reach 73.41%，and the polysaccharide loss was 25.92%.The IC50 of undeproteinized Adiantum capillus-veneris polysaccharides for α-glucosidase and DPPH radicals were 0.31,0.81 mg·mL-1，respectively.The IC50 of clematis Adiantum capillus-veneris polysaccharides for α-glucosidase and DPPH radicals were 0.26,0.59 mg·mL-1，respectively，which increased by 0.05,0.22 mg·mL-1 compared with undeproteinized proteins，respectively，which proved that the removal of protein by Sevage method could effectively improve the ability of Adiantum capillus-veneris polysaccharides to inhibit α-glucosidase and antioxidant capacity.

References:

［1］汪金梅，于旭东，蔡泽坪，等.铁线蕨属植物的研究进展［J］.分子植物育种，2019，17(1):255-263.［2］国家中医药管理局《中华草本》编委会，中华草本（第二册）［M］.上海:上海科学技术出版社，1999.［3］KHAN M F，TANG H，LYLES J T，et al.Antibacterial properties of medicinal plants from pakistan against multidrug-resistant ESKAPE pathogens［J］.Frontiers in Pharmacology，2018，9(2):815.［4］呼凤兰，胡诗琪.吕梁红枣多糖的提取及其抗氧化性研究［J］.北方园艺，2022(22):91-96.［5］KASABRI V，ALHALLAQ E K，BUSTANJI Y K，et al.Antiobesity and antihyperglycaemic effects of Adiantum capillus-veneris extracts:In vitro and in vivo evaluations［J］.Pharmaceutical Biology，2016，9(1):164-172.［6］何伟，孙自学，王光策.磁振磁电治疗仪联合前列倍喜胶囊治疗慢性前列腺炎/慢性骨盆疼痛综合征的临床疗效观察［J］.中华男科学杂志，2020，26(5):452-456.［7］牛小花，陈洪源.3种荷叶铁线蕨提取物抑菌活性研究［J］.内蒙古中医药，2017，36(10):130.［8］迪那拉·恰热甫汗，太力艾提·吐尔洪，热孜艳木·亚森，等.复方铁线蕨颗粒中总黄酮和总酚的提取及其对宫颈癌细胞的作用［J］.食品安全质量检测学报，2020，11(2):409-416.［9］YU Y，SHEN M，SONG Q，et al.Biological activities and pharmaceutical applications of polysaccharide from natural resources:A review［J］.Carbohydr Polym.2018，183(1):91-101.［10］王歆彤，李朋月，吴兰芳，等.知母多糖复合酶提取工艺优化及其免疫活性［J］.食品工业科技，2022，43(11):218-227.［11］LI W，HU X，WANG S，et al.Characterization and anti-tumor bioactivity of astragalus polysaccharides by immunomodulation［J］.Int J Biol Macromol，2020，145(15):985-997.［12］李文娟，初悦雷，潘雨欣，等.白扁豆多糖通过HPA轴介导降血糖的作用机制［J］.食品工业科技，2022，43(7):361-367.［13］杨庆伟，王芃，全迎萍.灰树花子实体多糖硫酸酯化及抗凝血活性研究［J］.北京联合大学学报，2022，36(2):52-56.［14］ZHOU S，HUANG G.Preparation，structure and activity of polysaccharide phosphate esters［J］.Biomed Pharmacother，2021，144:112332.［15］赵丽平，刘琦，蔡静，等.刺槐花多糖蛋白质脱除工艺优化及生物活性测试［J］.中国食品添加剂，2022，33(9):63-68.［16］王莹，邢晓玲，李屿君，等.香菇多糖脱蛋白工艺及其抗氧化活性研究［J］.食品研究与开发，2020，41(14):98-103.［17］亓希武，房海灵，陈泽群，等.桃胶多糖脱蛋白工艺研究［J］.现代食品，2021(8):104-107.［18］华波.重庆特有植物荷叶铁线蕨Adiantum reniforme var.sinense多糖和黄酮的提取、抗氧化及抑菌特性研究［D］.重庆:西南大学，2011.［19］殷凯，李湘洲，张胜，等.长沙青皮竹多糖脱蛋白质方法研究［J］.食品与机械，2013，29(6):176-178.［20］蔡月琴，刘艺敏，凡莉莉，等.响应面法优化巴戟天多糖提取工艺［J］.森林与环境学报，2019，39(3):303-309.［21］陈越，宋振康，张海悦.三氯乙酸法脱除龙葵果多糖中蛋白质的工艺优化［J］.食品与发酵工业，2020，46(24):198-203.［22］尹延霞，朱奇峰，刘汉杰，等.中心组合实验设计响应面法优化α淀粉酶抑制剂筛选条件［J］.西南师范大学学报（自然科学版），2015，40(4):83-88.［23］GHANI U，NUR-E-ALAM M，YOUSAF M，et al.Natural flavonoid α-glucosidase inhibitors from Retama raetam:Enzyme inhibition and molecular docking reveal important interactions with the enzyme active site［J］.Bioorg Chem，2019，87:736-742.［24］李美萍，侯健，刘燕，等.细叶韭花醇提物体外抗氧化及对α-葡萄糖苷酶抑制作用的研究［J］.中国调味品，2022，47(2):30-34.［25］胡文兵，杨占威，陈慧，等.Plackett-Burman和Box-Behnken试验设计优化超声波-酶法提取青钱柳多糖工艺及结构初探［J］.天然产物研究与开发，2017，29(4):671-679.［26］ZHANG R，MA C，WEI Y，et al.Isolation，purification，structural characteristics，pharmacological activities，and combined action of Hedyotis diffusa polysaccharides:A review［J］.Int J Biol Macromol，2021，183(31):119-131.［27］张勇，周丽明，黄章平.比清除率衡量Sevage法脱茶籽多糖蛋白的效果［J］.南方农业学报，2016，47(1):107-111.［28］华波.重庆特有植物荷叶铁线蕨Adiantum reniforme var.sinense多糖和黄酮的提取、抗氧化及抑菌特性研究［D］.重庆:西南大学，2011.

Memo

Memo:
-
Last Update: 2023-11-30