|Table of Contents|

Optimization of Reflux Extraction Process and Antioxidant Activity of Total Flavonoids From Pyrrosia lingua by Response Surface Method

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年19
Page:
78-84
Research Field:
Publishing date:

Info

Title:
Optimization of Reflux Extraction Process and Antioxidant Activity of Total Flavonoids From Pyrrosia lingua by Response Surface Method
Author(s):
ZHANG Minjie; YANG Wude; YU Xiang; LUO Guoyong; ZHANG Yan; LIANG Shanshan
(College of Pharmacy,Guizhou University of Traditional Chinese Medicine/Key Laboratory of Traditional Chinese Medicine,Guiyang,Guizhou 550002)
Keywords:
Pyrrosia lingua; total flavonoids; extraction technology; response surface method; antioxidant activity
PACS:
R 284
DOI:
10.11937/bfyy.20230194
Abstract:
Taking dried leaves of Pyrrosia lingua as test materials,the extraction process of total flavonoids from dried leaves of Pyrrosia lingua was optimized by single factor experiment combined with response surface methodology,and the antioxidant activity of the extract against free radicals such as ABTS+·,DPPH· and OH· was studied,in order to provide reference for the followup extraction process and the development and utilization of antioxidant capacity.The results showed that the optimum extraction conditions of total flavonoids were as follows,ratio of material to liquid was 1∶40 g·mL-1,extraction time was 32 minutes,extraction temperature was 80 ℃.After three experiments,the content of total flavonoids was (120.41±1.25) mg·g-1 (RSD=1.37%,n=3).The in vitro test showed that the total flavonoids of Pyrrosia lingua had certain scavenging ability on ABTS+·,DPPH·,OH·.BoxBehnken response surface method could be used to optimize the extraction process of total flavonoids from Pyrrosia lingua,and the extraction process was stable and feasible.Under these conditions,the extract of total flavonoids from Pyrrosia lingua had good antioxidant activity.

References:

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Last Update: 2023-11-29