|Table of Contents|

AtWUS Promotes High Frequency Somatic Embryogenesis Potato Onion（Allium cepa L.var.Aggregatum G.Don）

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年1
Page:
24-30
Research Field:
Publishing date:

Info

Title:
AtWUS Promotes High Frequency Somatic Embryogenesis Potato Onion（Allium cepa L.var.Aggregatum G.Don）
Author(s):
XU Qijiang1; 2; HUANG Junhui3; HE Tao2; XING Zhiyong1; 2; YANG Anni1; HUANG Yuntong1; 2
(1.Modern Industrial College of Biomedicine and Great Health，Youjiang Medical University for Nationalities，Baise，Guangxi 533000；2.School of Laboratory Medicine，Youjiang Medical University for Nationalities，Baise，Guangxi 533000；3.Baise Institute of Scientific and Technical Information，Baise，Guangxi 533000)
Keywords:
potato onion (Allium cepa var.aggregatum Don); shoot apex explants; callus; somatic embryogenesis; AtWUS gene
PACS:
S 633.2
DOI:
10.11937/bfyy.20230079
Abstract:
Taking callus induced from shoot apex explants of potato onion (Allium cepa var.aggregatum Don) as test material，the foreign gene AtWUS genetic transformation was carried out by the agrobacterium-mediated transformation method with potato onion callus.The effects of AtWUS on somatic embryogenesis (SE) of potato onion calluswas studied，in order to develop a high-frequency SE regeneration systemvia over-expressing AtWUS from in potato onion callus.The results showed that overexpression of the AtWUS gene incallus promoted，as the percentage of explants giving rise to embryogenic tissues was significantly higher (×6) when AtWUS gene was over-expressed than in the control.Bipolar somatic embryos resulted 91% complete plant regeneration.RT-PCR analysis showed that AtWUS gene was highly expressed in somatic embryos and transgenic plants.

References:

［1］ANGIOSPERM PHYLOGENY GROUP.An update of the angiosperm phylogeny group classification for the orders and families of flowering plants:APGⅢ［J］.Botanical Journal of the Linnean Society,2009,161:105-121.［2］FRITCH R M,FRIESEN N.Evolution,domestication and taxonomy［M］//RABINOWITCH H D,CURRAH L.Allium Crop Science：Recent Advances.Wallingford：CABI Publishing,2002.［3］黄云彤,娄虎,刘学,等.分蘖洋葱鳞茎总黄酮提取工艺及抗氧化活性［J］.北方园艺,2019(14):106-116.［4］RABINOWITCH H D.Onions and allied crops：Volume I：Botany，physiology，and genetics［M］.1st ed.Florida：CRC Press，1990.［5］LEINO M W,SOLBERG S ,TUNSET H M,et al.Patterns of exchange of multiplying onion (Allium cepa L.Aggregatum-Group) in Fennoscandian home gardens［J］.Economic Botany,2018,72(3):346-356.［6］徐启江,丁国华,陈典.黑龙江省分蘖洋葱病毒病原的初步鉴定［J］.西北农林科技大学学报,2003,31(2):55-58［7］徐启江,陈典,张云修.分蘖洋葱茎尖培养脱毒苗技术研究［J］.西北农林科技大学学报,2002,30(2):101-106［8］LOU H,LIU Z Q,XU Q J.Detection and elimination of shallot latent virus and onion yellow dwarf virus for potato onion (Allium cepaL.var.aggregatumDon)［J］.Annals of Applied Biology,2023,182(1):112-120.［9］王健鹂,张悦,王秀峰,等.不同培养条件对分蘖洋葱茎尖脱毒效果的影响［J］,辣椒杂志,2017(4):31-35.［10］陈典,徐启江.分蘖洋葱愈伤组织诱导及植株再生［J］.园艺学报,2001,28(4):359-360.［11］徐启江,杨守志,池春玉,等.分蘖洋葱试管鳞茎微繁技术研究［J］.植物学通报,2003,20(1):80-84.［12］宋策,陈典,梁誉,等.秋水仙素离体诱导分蘖洋葱茎尖多倍体的研究［J］.吉林农业大学学报,2016(6):675-680.［13］OSORIO-MONTALVO P,SENZ-CARBONELL L,DE-LA-PEA C.5-Azacytidine:A promoter of epigenetic changes in the quest to improve plant somatic embryogenesis［J］.International Journal of Molecular Sciences,2018,19(10):3182.［14］VON ARNOLD S,SABALA I,BOZHKOV P,et al.Developmental pathways of somatic embryogenesis［J］.Plant Cell Tissue Organ Culture,2002,69:233-249.［15］SILVA-CARDOSO I M A,MEIRA F S,GOMES A C M M,et al.Histology histochemistry and ultrastructure of pre-embryogenic cells determined for direct somatic embryogenesis in the palm tree Syagrus oleracea［J］.Physiologia Plant,2020,168(4):845-875.［16］LOYOLA-VARGAS V M.The History of somatic embryogenesis.In somatic embryogenesis：Fundamental aspects and applications［M］.Switzerland：Springer Cham，2016.［17］ZIMMERMAN J L.Somatic embryogenesis：A model for early development in higher plants［J］.Plant Cell,1993(5):1411-1423.［18］FLOREZ S L,ERWIN R L,MAXIMOVA S N,et al.Enhanced somatic embryogenesis in Theobroma cacao using the homologous BABY BOOM transcription factor［J］.BMC Plant Biology,2015(15):121.［19］MNDEZ-HERNNDEZ H A,LEDEZMA-RODRGUEZ M,AVILEZ-MONTALVO R N,et al.Signaling overview of plant somatic embryogenesis［J］.Frontiers in Plant Science,2019(10):77.［20］HORSTMAN A,BEMER M,BOUTILIER K A.Transcriptional view on somatic embryogenesis［J］.Regeneration,2017(4):201-216.［21］SALAN C,LEPINIEC L,DUBREUCQ B.Genetic and molecular control of somatic embryogenesis［J］.Plants,2021(10):1467.［22］WANG F X,SHANG G D，WANG J W.Towards a hierarchical gene regulatory network underlying somatic embryogenesis［J］.Trends Plant Sci,2022(27):1206-1217.［23］MAYER K F,SCHOOF H,HAECKER A,et al.Role of WUSCHEL in regulating stem cell fate in the Arabidopsis shoot meristem［J］.Cell,1998,95(6):805-815.［24］FLETCHER C J.The CLV-WUS stem cell signaling pathway:A roadmap to crop yield optimization［J］.Plants (Basel),2018,7(4):87.［25］RASHID S Z,YAMAJI N,KYO M.Shoot formation from root tip region：A developmental alteration by WUS in transgenic tobacco［J］.Plant Cell Report,2007(26):1449-1455.［26］ARROYO-HERRERA A,KU-GONZALEZ A,CANCHE-MOO R,et al.Expression of WUSCHEL in Coffea canephora causes ectopic morphogenesis and increases somatic embryogenesis［J］.Plant Cell Tissue and Organ Culture,2008,94:171-180.［27］SOLS-RAMOS L Y,GONZLEZ-ESTRADA T,NAHUATH-DZIB S,et al.Overexpression of WUSCHEL in C.chinense causes ectopic morphogenesi［J］.Plant Cell Tissue and Organ Culture,2009,96:279-287.［28］KLIMASZEWSKA K,PELLETIER G,OVERTON C,et al.Hormonally regulated overexpression of Arabidopsis WUS and conifer LEC1 (CHAP3A) in transgenic white spruce：Implications for somatic embryo development and somatic seedling growth［J］.Plant Cell Report,2010(29):723-734.［29］BOUCHABKE-COUSSA O,OBELLIANNE M,LINDERME D,et al.Wuschel overexpression promotes somatic embryogenesis and induces organogenesis in cotton (Gossypium hirsutum L.) tissues cultured in vitro［J］.Plant Cell Report,2013(32):675-686.［30］ZHENG W,ZHANG X,YANG Z,et al.AtWuschel Promotes formation of the embryogenic callus in Gossypium hirsutum［J］.PLoS One,2014,9(1):e87502.［31］TVOROGOVA V E,FEDOROVA Y E,POTSENKOVSKAYA E A,et al.The WUSCHEL-related homeobox transcription factor MtWOX9-1 stimulates somatic embryogenesis in Medicago truncatula［J］.Plant Cell Tissue Organ Culture,2019(3):517-527.［32］KADRI A，GRENIER de MARCH G，et al.WUSCHEL Overexpression promotes callogenesis and somatic embryogenesis in Medicago truncatula Gaertn［J］.Plants (Basel),2021,10(4):715.［33］DEBLAERE R,BYTEBIER B,de GREVE H,et al.Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants［J］.Nucleic Acids Research，1985,13(13):4777-4788.［34］WOJCIK A M,WOJCIKOWSKA B,GAJ M D.Current perspectives on the auxin-mediated genetic network that controls the induction of somatic embryogenesis in plants［J］.International Journal of Molecular Sciences,2020(21):1333.［35］SALAUN C，LEPINIEC L，DUBREUCQ B.Genetic and molecular control of somatic embryogenesis［J］.Plants (basel),2021(10):1467.［36］MOZGOV I,MUOZ-VIANA R,HENNIG L.PRC2 represses hormone-induced somatic embryogenesis in vegetative tissue of Arabidopsis thaliana［J］.PLoS Genetics,2017(13):e1006562.［37］XIAO Y,CHEN Y,DING Y,et al.Effects of GhWUS from upland cotton (Gossypium hirsutum L.) on somatic embryogenesis and shoot regeneration［J］.Plant Science,2048,270:157-165.［38］MAYER K F,SCHOOF H,HAECKER A,et al.Role of WUSCHEL in regulating stem cell fate in the Arabidopsis shoot meristem［J］.Cell,1998,95:805-815.［39］LAUX T,MAYER K F,BERGER J,et al.The WUSCHEL gene is required for shoot and floral meristem integrity in Arabidopsis［J］.Development,1996,122:87-96.［40］ZHANG T Q,LIAN H,ZHOU C M,et al.A two-step model for de novo activation of WUSCHEL during plant shoot regeneration［J］.Plant Cell,2017(29):1073-1087.［41］ELHITI M,STASOLLA C.Transduction of signals during somatic embryogenesis［J］.Plants (Basel),2022，11(2):178.［42］LOU H,HUANG Y,WANG W,et al.Overexpression of the AtWUSCHEL gene promotes somatic embryogenesis and lateral branch formation in birch (Betula platyphylla Suk.)［J］.Plant Cell Tissue Organ Culture,2022,150:371-383.［43］FAMBRINI M,USAI G,PUGLIESI C.Induction of somatic embryogenesis in plants：Different players and focus on WUSCHEL and WUS-RELATED HOMEOBOX (WOX) transcription factors［J］.International Journal of Molecular Sciences,2022,23(24):15950.

Memo

Memo:
-
Last Update: 2024-01-22