|Table of Contents|

Identification and Biological Characteristics of Root Rot Pathogen of Ligusticum jeholense

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年14
Page:
118-124
Research Field:
Publishing date:

Info

Title:
Identification and Biological Characteristics of Root Rot Pathogen of Ligusticum jeholense
Author(s):
ZHANG Tianjing1; 2; SUN Wensong1; 2; SHEN Baoyu1; 2; LIU Kun1; 2
(1.Liaoning Research Institute of Cash Crops,Liaoyang,Liaoning 111000;2.Institute of Medicinal Plants,Liaoning Academy of Agricultural Sciences,Liaoyang,Liaoning 111000)
Keywords:
Ligusticum jeholense; root rot; Fusarium solani; biological characteristics
PACS:
-
DOI:
10.11937/bfyy.20225201
Abstract:
Taking diseased plants with typical symptoms as test materials,pathogen isolation was carried out by means of diseased tissue isolation method.Koch′s rule was used to prove the pathogenicity of fungal strain,in order to explore the main pathogens of Ligusticum jeholense root rot disease and its biological characteristics.The results showed that based on the morphological observation and sequence analyses of translation elongation factor EF-1α, Fusarium solani was identified as the pathogen.The biological characteristics showed that different media had significant effects on the growth and mycelial density of pathogen.The suitable medium for Fusarium solani growth was PDA,the colony diameter reached 85 mm in 7 days,and the colonies were dense.The growth of mycelia with sucrose as carbon source was faster than that with soluble starch，and the ability of pathogen to utilize lactose was poor.The most suitable nitrogen source was beef extract，followed by urea and potassium nitrate.The suitable temperature for the mycelium growth was 20-30 ℃，the optimum temperature was 28-30 ℃.The optimum pH was 6.The light treatment had an influence on the growth of mycelium，suitable light condition for the mycelium growth was 24 hour continuous dark.

References:

[1]于英,张永刚,刘国辉,等.辽藁本主要生物学特性研究[J].吉林农业大学学报,2005,27(4):401-404,407.[2]卢星原,孙启时,查美娜,等.辽藁本化学成分的分离与鉴别[J].沈阳药科大学学报,2010,27(6):434-439.[3]国家药典委员会.中国药典(一部)[M].北京:中国医药科技出版社,2020.[4]孙文松,李玲,于春雷,等.辽藁本生产研究现状及发展对策[J].辽宁省农业科学,2018(5):60-63.[5]刘莹,李旭,孙文松.辽藁本常见病虫害及综合防控技术[J].园艺与种苗,2021,41(4):35-36.[6]董汉松.植病研究法[M].北京:中国农业出版社,2012.[7]方中达.植病研究法[M].3版,北京:中国农业出版社,1988.[8]LESLIE J F，SUMMERELL B A.The Fusarium laboratory manual[M].USA:Blackwell Publishing,2006.[9]胡兰,姜钰,徐秀德.基于形态学和EF-1α序列特征的我国高粱子粒寄藏镰孢菌种群鉴定[J].作物杂志,2013(4):129-132.[10]COLLINS C H，LYNE P M，GRANGE J M,et al.Collins ＆ Lyne′s microbiological methods[M].Oxfod:Oxford University Press Inc,2004.[11]张雁,袁泉,刘莉莉,等.拮抗尖孢镰刀菌的蓝莓菌根真菌的筛选和鉴定[J].巢湖学院学报,2019,21(3):78-88.[12]程鸿燕,张大琪,黄斌,等.微生物菌肥对熏蒸剂处理后土壤微生态的影响研究进展[J].农药学学报,2020,22(5):734-741.[13]穆向荣,马逾英,杨枝中,等.药用植物根腐病防治的研究进展[J].中药与临床,2014,5(2):5-8.[14]姜竹,李晶.中药材土传病害生物防治研究进展[J].现代农业科技,2009(24):152-153.[15]JANG C S，LIM J H，SEO M W，et al.Direct detection of Cylindrocarpon destructans，root rot pathogen of ginseng by nested PCR from soil samples[J].Mycobiology，2010,38(1)：33-38.[16]SUMMERELL B A，SALLEH B，LESLIE J F.A utilitarian approach to Fusarium identification[J].Plant Disease，2003，87(2):117-128．[17]TEHRANI M M，NASR E M，MOUSAVI A，et al.Regulation of related genes promoting resistant in Iris against root rot disease， Fusarium oxysporum f.sp.gladioli[J].Genomics,2020,112(5)：3013.[18]高芬,任小霞,王梦亮,等.中草药根腐病及其微生物防治研究进展[J].中国中药杂志,2015,40(21)：4122-4126.[19]闫文雪,石延霞,柴阿丽,等.豇豆根腐病病原菌鉴定[J].植物保护学报,2019,46(3)：717．[20]陈为京,郭峰,陈建爱,等.连作花生根腐病镰刀菌分离与对峙培养[J].花生学报，2018，47(2):47-51.[21]罗文富,喻盛甫,贺承福,等.三七根腐病病原及复合侵染的研究[J].植物病理学报,1997,27(1)：85-91.[22]冯茜.川芎根腐病病原鉴定、生物学特性和同工酶研究[D].雅安:四川农业大学,2008.[23]李勇,刘时轮,杨成民,等.北京地区柴胡根腐病的病原菌鉴定[J].植物病理学报,2009，39(3)：314-317.[24]XIAO R F,ZHU Y J,WANG J P，et al.First report of Fusarium solani causing root rot on Levisticum officinale in China[J].Plant Disease，2017,101(4)：630.[25]连芸芸,李焕宇,李惠霞,等.甘肃省庆阳市辣椒镰孢菌根腐病病原鉴定及生物学特性[J].甘肃农业大学学报,2021,56(5):55-61,68.[26]柏玉晶,张贞明,姚玉玲,等.紫花苜蓿根腐新病原厚垣镰孢菌生物学特性研究[J].草原与草坪,2016,36(6):49-53.[27]林清洪,林光荣,曾碧玉,等.蝴蝶兰叶基腐病病原鉴定及生物学特性[J].热带作物学报,2007,28(4):93-97.[28]朱孟烽,吴伟怀,贺春萍,等.咖啡腐皮镰孢黑果病病原鉴定及其生物学特性测定[J].热带作物学报,2021,42(3):822-829.[29]耿丽华,白庆荣,王建设,等.甜瓜镰孢根腐病菌生物学特性研究[J].中国农学通报,2010,26(7):229-232.[30]贵晓荷.紫花苜蓿根腐病病原镰刀菌的生物学特性研究[D].兰州:兰州大学,2019.[31]沈丽淘,李平,王学贵,等.山药根腐病菌(Fusarium solani)的生物学特性[J].四川农业大学学报,2012,30(3):313-318.[32]刘丽云,刘晓林,刘志恒,等.辣椒根腐病菌生物学特性研究[J].沈阳农业大学学报,2007(1):54-58.[33]姚锦爱,黄鹏,陈峰,等.建兰茎腐病原菌尖孢镰刀菌的生物学特性研究[J].福建农业学报,2018,33(2)：190.[34]杨静美,陈健,罗金棠,等.番木瓜茄病镰刀菌的生物学特性研究[J].中国热带农业,2011(1)：56.[35]XU C G,ZHANG H J,ZHOU Z S,et al.Identification and distribution of Botryosphaeriaceae species associated with blueberry stem blight in China[J].European Journal of Plant Pathology,2015，143(4):737.

Memo

Memo:
-
Last Update: 2023-08-29