|Table of Contents|

Identification and Analysis of the Transcription Factor and Laccase Expression Regulators Based on Genome and Transcriptome Databases in Phlebopus portentosus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年16
Page:
108-116
Research Field:
Publishing date:

Info

Title:
Identification and Analysis of the Transcription Factor and Laccase Expression Regulators Based on Genome and Transcriptome Databases in Phlebopus portentosus
Author(s):
ZHOU Chenli1GUO Ting1JI Guangyan2JI Kaiping2BAO Dapeng1YANG Ruiheng1
(1.Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences/Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs/National Engineering Research Center of Edible Fungi/Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403;2.Hongzhen Agricultural Science and Technology Co.Ltd.,Jinghong,Yunnan 666100)
Keywords:
omicstranscription factorslaccaseweighted gene coexpression regulatory network
PACS:
-
DOI:
10.11937/bfyy.20224857
Abstract:
Phlebopus portentosus was used as the test materials.Genome and transcriptome were conducted to identify transcription factors,expression patterns at different developmental stages,and regulatory effects on laccases,in order to provide reference for the research on the regulation mechanism of the fruiting body development of Phlebopus portentosus.The results showed that a total of 144 transcription factor were predicted in the genome,belonging to 10 different families.The C2H2 transcription factors were the most abundant,followed by C3H transcription factors,which were involved in the regulation of catalytic activity,biological and metabolic process.The expression patterns of transcription factors in mycelium stage,primordial stage and harvest stage were different,regulating different biological processes.Six laccase genes were detected through genome annotation and domain analysis.According to the expression at three different development stages,they were divided into four different families,never expressed,highly expressed at the mycelium stage,highly expressed at the primordia and fruiting body stage and stably expressed.There were 6 transcription factors that regulate 4 laccase genes,of which SRF transcription factors control the highly expressed laccase genes at the mycelium stage with positive correlation,Sox1 transcription factors control the expression of laccase in the primordial fruiting body with negative correlation.Transcription factors and laccase played a certain role in the regulation of fruiting body development.

References:

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Last Update: 2023-09-27