|Table of Contents|

Analysis on the Structure and Function of Bacterial Community in the Soil Around the Root of Handkea utriformis Based on 16S rDNA High Throughput Sequencing Technology

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年20
Page:
61-68
Research Field:
Publishing date:

Info

Title:
Analysis on the Structure and Function of Bacterial Community in the Soil Around the Root of Handkea utriformis Based on 16S rDNA High Throughput Sequencing Technology
Author(s):
YIN Shuli1; LIANG Ran2; MA Lulu1; ZHANG Junhua3; WEN Peiyao3; WANG Yiming3
(1.Biology Institute,Hebei Academy of Sciences，Shijiazhuang，Hebei 050081；2.Agricultural Technology Extension Center of Luquan District，Shijiazhuang，Hebei 050200；3.Hebei North University，Zhangjiakou，Hebei 075032)
Keywords:
Handkea utriformis; high throughput sequencing; bacterial community structure; functional groups
PACS:
Q 939.96
DOI:
10.11937/bfyy.20224757
Abstract:
Taking degraded soil and normal soil from Shuangyashan City,Heilongjiang Province as test materials,the community structure and functional differences of 16S rDNA V4-V5 bacterial communities in degraded soil and normal soil samples were studied using Illumina high-throughput sequencing method,in order to investigate the reasons why Handkea utriformis can not grow in degraded soil and provide reference for artificial domestication of Handkea utriformis.The results showed that the operational taxonomic units belong to 2 kingdoms，44 phyla，128 classes，299 orders，508 families，1 048 genera.Richness index (Chao1 and ACE) and diversity index (Shannon and InvSimpson) of degenerated soil bacterial communities all decreased.In the degenerated soil，the relative abundances of Bacteroidetes，Gemmatimonadetes，Planctomycetes，Pseudarthrobacter，RB41，Terrimonas decreased，and the relative abundances of Verrucomicrobia，Acidothermus，Rhodanobacter increased.LEfSe analysis results showed that in CK soil，12 dominant bacterias were Alphaproteobacteria，AD3，Acidobacteria，Ktedonobacterales，Rhizobiales，Gaiellales，Micropepsales，Micropepsaceae，Ktedonobacteraceae，Rhodanobacteraceae，HSB OF53-F07，Candidatus Solibacter.2 metabolic pathways of the primary functional layers were the metabolism and organic system，6 sub function layers were biosynthesis of other secondary metabolites，energy metabolism，metabolism of cofactors and vitamins，metabolism of terpenoids and polyketones，circulatory system and digestive system.Compared with normal soil，3 primary metabolic pathways and 8 secondary metabolic pathways were reduced.The structural changes of bacterial community structure in the degenerated soil and the accompanying changes in metabolic function were the important reasons for the inability of the Handkea utriformis continue to grow.

References:

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Last Update: 2023-11-30