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Study on the Proliferation and Differentiation of Rhizomes in the Hybrid Progeny of Hybrid Orchid ‘Hongmeiren’×Cymbidium kanran ‘Yangguifei’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年14
Page:
63-70
Research Field:
Publishing date:

Info

Title:
Study on the Proliferation and Differentiation of Rhizomes in the Hybrid Progeny of Hybrid Orchid ‘Hongmeiren’×Cymbidium kanran ‘Yangguifei’
Author(s):
YANG Songmin1; 2; 3; TANG Ruonan1; 2; 3; CHENG Mengya1; 2; 3; NIU Muqi1; 2; 3; CHEN Heming4; PENG Donghui1; 2; 3
(1.College of Landscape Architecture and Arts,Fujian Agriculture and Forestry University，Fuzhou,Fujian 350002； 2.Fujian Engineering Research Center of Ornamental Plant Germplasm Resources Innovation and Application，Fuzhou，Fujian 350002；3.Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization，Fujian Agriculture and Forestry University，Fuzhou,Fujian 350002;
Keywords:
hybrid orchid; tissue culture; rhizome; proliferation; differentiation
PACS:
-
DOI:
10.11937/bfyy.20224732
Abstract:
Taking hybrid orchid ‘Hongmeiren’×Cymbidium kanran ‘Yangguifei’ of rhizomes as experimental material，using L9(34) orthogonal experiment method，the effects of 6-BA，NAA，TDZ on different photoperiod and basic culture medium of rhizomes proliferation，leaf bud differentiation and flower bud differentiation were studied，in order to explore the hybrid laingen stems proliferation and differentiation of suitable conditions.The results showed that the best formula of rhizome proliferation,2.0 g?L-1 Huabao 1+1.0 g?L-1 Huabao 2+1.0 mg?L-1 TDZ+1.0 mg?L-1 NAA+0.1g?L-1 activated carbon+40.0 g?L-1 sucrose+7.0 g?L-1 agar+6.0 g?L-1 proteinpeptone+40.0 g?L-1 banana+40.0 g?L-1 potato;the proliferation coefficient was highest at 12 hours/12 hours (light/dark) and the fastest growth rate at 10 hours/14 hours (light/dark).The best formula of leaf bud differentiation,2.0 g?L-1 Huabao 1+1.0 g?L-1 Huabao 2+5.0 mg?L-1 6-BA+1.0 mg?L-1 NAA+0.1 g?L-1activated carbon+40.0 g?L-1 sucrose+7.0 g?L-1 agar+6.0 g?L-1 peptone+50.0 mg?L-1 coconut milk.In MS+5.0 mg?L-1 6-BA+1.0 mg?L-1 NAA+0.1 g?L-1 activated carbon+40.0 g?L-1 sucrose+7.0 g?L-1 agar+6.0 g?L-1 proteinpeptone+40.0 g?L-1 banana+40.0 g?L-1 potato，the rhizome produced flower buds.

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Last Update: 2023-08-29