|Table of Contents|

Genetic Diversity Analysis of Phallus impudicus Strains Based on ISSR Molecular Markers

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年14
Page:
133-139
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of Phallus impudicus Strains Based on ISSR Molecular Markers
Author(s):
LU Yingying1; 2; 3; 4; LIU Hongyu1; 2; 3; 4; HUANG Wanbing1; 2; 3; 4; GUI Yang1; 2; 3; 4; ZHU Guosheng1; 2; 3; 4
(1.Institute of Crop Germplasm Resources，Guizhou Academy of Agricultural Sciences，Guiyang，Guizhou 550006； 2.Guizhou Key Laboratory of Edible Fungi Breeding，Guiyang，Guizhou 550006;3.Resource Breeding Function Laboratory of Guizhou Modern Agricultural Industry Technology System of Edible Fungi，Guiyang，Guizhou 550006;4.Resource Breeding Laboratory of Guizhou Engineering Research Center of Edible Fungi，Guiyang，Guizhou 550006)
Keywords:
Phallus impudicus; ISSR; genetic diversity
PACS:
-
DOI:
10.11937/bfyy.20224705
Abstract:
The genetic diversity of 20 Phallus impudicus species from different regions was analyzed using Inter simple sequence repeat (ISSR) molecular markers.From 78 primers，the primers with clear bands，high repeatability and high specificity were screened and amplified in the tested strains of Phallus impudicus.The UPGMA method of NTSYS 2.10e cluster analysis software was used for cluster analysis，and the ISSR statistics of 20 Phallus impudicus samples were analyzed by PopGen32,in order to evaluate the genetic diversity of Phallus impudicus germplasm resources by molecular markers and provide high quality materials for genetic breeding.The results showed that a total of 24 ISSR primers with polymorphism were screened out，and 788 ISSR bands were amplified by PCR，included 768 polymorphism bands，with the proportion of polymorphic loci reaching 97.5%；genetic diversity analysis showed that the average allele (Na) was 1.991 3，the average effective allele number (Ne) was 1.585 6，the average Nei′s gene diversity index (He) was 0.343 8，and the average Shannon information index (I) was 0.516 1.When the genetic similarity coefficient was 0.67，the 20 strains could be divided into three groups.In conclusion，there were abundant genetic diversity and complex genetic relationship among the 20 species.Due to the frequent circulation of germplasm from different sources，the genetic relationship was complicated，and the selection and purification cycle of breeding parents was long.

References:

[1]郑秀艳,孟繁博,邬彩灵,等.黔产白鬼笔不同部位品质分析与评价[J].现代食品科技,2021,37(12):23-32.[2]李文力,黎璐,汤洪敏.黔产白鬼笔不同部位提取物的成分分析[J].食品科学,2016,37(2):72-76.[3]桂阳,龚光禄,卢颖颖,等.白鬼笔出菇特性研究[J].食用菌,2013,35(3):12-14.[4]宋笛.响应面优化微波辅助提取白鬼笔多糖及其抗氧化和抗糖化能力[J].食品研究与开发,2021,42(20):130-136.[5]易雪倩,徐宁,王小艳,等.一种鬼笔属真菌分离鉴定和挥发性成分分析[J].中国蔬菜,2022(7):78-84.[6]李永齐,王芬,廖头根,等.白鬼笔菌托多糖作为烟草保润剂的性能评价[J].食药用菌,2019,27(6):383-389.[7]UKO V,AKUNOVICH A,STOWSKI A,等.白鬼笔蘑菇菌丝体多糖：免疫调节和伤口愈合特性（英文）[J].现代食品科技,2019,35(9):30-37.[8]武海月,赵爽,刘宇,等.榆黄菇遗传多样性的ISSR和SRAP综合分析[J].生物技术通报,2018,34(4):121-126.[9]杨和川,苏文英,谭一罗,等.基于ISSR分子标记技术的杏鲍菇种质资源评价[J].西南农业学报,2019,32(7):1480-1486.[10]宋莹,刘俊杰,刘岩岩,等.辽宁省主栽香菇菌株ISSR遗传差异性分析[J].北方园艺,2017(5):82-85.[11]罗燕娜,王静静,李旭东,等.双孢蘑菇A15菌株的ISSR分子标记聚类分析[J].分子植物育种,2022,20(2):473-478.[12]柯丽娜,黄艺宁,袁滨,等.主栽毛木耳菌株农艺性状及ISSR遗传分析[J].福建农业学报,2021,36(7):771-778.[13]张彬彬,王震,刘保卫,等.30个野生灵芝菌株遗传多样性及农艺性状评价[J].江苏农业科学,2022,50(2):108-114.[14]汪茜,吴圣进,韦仕岩,等.金福菇菌株遗传多样性的ISSR分析[J].西南农业学报,2014,27(2):768-771.[15]王波,鲜灵.金针菇菌株遗传多样性的ISSR分析[J].西南农业学报,2013,26(4):1593-1599.[16]杨杰,张进强,周涛,等.蜜环菌和冬荪菌侵染与降解菌材的比较研究[J].中国中药杂志,2020,45(3):472-477.[17]张进强,江维克,郭兰萍,等.天麻种植旧菌材的资源再利用探索[J].中国中药杂志,2022,47(9):2309-2314.[18]刘鹏,曹永直,彭成江,等.利用天麻空窝和废菌材栽培白鬼笔技术[J].食用菌,2022,44(1):49-51.[19]王鸿磊,丁强,吕蔚,等.我国双孢菇菌株与国外引进菌株间亲缘关系的ISSR分析[J].北方园艺,2014(11):96-99.[20]贾培松,努尔孜亚?亚力买买提,罗影,等.新疆塔城地区野生阿魏菇菌株的遗传多样性分析[J].新疆农业科学,2016,53(10):1900-1906.[21]陈雪凤,郎宁,韦仕岩,等.8个反季节秀珍菇菌株栽培性状评价与遗传差异分析[J].西南农业学报,2019,32(8):1898-1903.[22]杨和川,苏文英,谭一罗,等.基于ISSR分子标记技术的杏鲍菇种质资源评价[J].西南农业学报,2019,32(7):1480-1486.[23]杨燕,田鸿,张小平,等.基于ITS和ISSR的羊肚菌种质资源遗传多样性分析[J].西南农业学报,2018,31(10):2004-2009.[24]谭艳,王波,赵瑞琳.金针菇菌株农艺性状评价与遗传差异分析[J].西南农业学报,2016,29(12):2965-2970.[25]王晓敏,吕瑞娜,李长田.应用SRAP、ISSR和TRAP标记构建金针菇分子遗传连锁图谱[J].分子植物育种,2020,18(13):4377-4383.[26]赵光辉,方洪枫,陈剑,等.20株草菇遗传多样性的分析[J].江苏农业科学,2020,48(16):194-197.

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Last Update: 2023-08-29