|Table of Contents|

Optimization of Protoplast Formation of Stropharia rugosoannulate by Response Surface Method

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年10
Page:
115-120
Research Field:
Publishing date:

Info

Title:
Optimization of Protoplast Formation of Stropharia rugosoannulate by Response Surface Method
Author(s):
LYU Ruiling; XU Siqi; QIN Lihao; CUI Gaoqin; ZHENG Yongliang
(College of Biology and Agricultural Resources,Huanggang Normal University/Hubei Key Laboratory for the Improvement of Economic Forest Germplasm and Comprehensive Utilization of Resources/Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains,Huanggang,Hubei 438000)
Keywords:
Stropharia rugosoannulate; protoplast; response surface method
PACS:
-
DOI:
10.11937/bfyy.20223993
Abstract:
The mycelium of Stropharia rugosoannulate was used as the research material.The reaction conditions were optimized by response surface method based on the single factor test with protoplast yield as response value,in order to provide reference for genetic improvement of S.rugosoannulata in the later stage.The results showed that after the optimization of response surface method,the preparation conditions for the protoplasts were obtained as follows,enzymolysis time was 2.5 hours,enzymolysis temperature was 32.2 ℃,and the mycelial age was 3.8 days,the maximum protoplast yield was 35.8×106 CFU?mL-1,Compared with the theoretical yield (35.15×106 CFU?mL-1),the deviation was small.Therefor,the optimum preparation conditions based on response surface method can obtain higher protoplast yield.

References:

[1]黄年来.大球盖菇的分类地位和特征特性[J].食用菌,1995,17(6):11.[2]于慧萍，胡思，黄文，等.干制方式对大球盖菇滋味物质的影响[J].食品工业科技,2021,42(9):251-256.[3]CHEN WC,LI W,WU D,et al.Characterization of novel umami-active peptides from Stropharia rugosoannulata mushroom and in silico study on action mechanism[J].Journal of Food Composition and Analysis,2022,110:104530.[4]洪琦,赵勇,陈明杰,等.大球盖菇菌渣原位还田对土壤有机质、酶活力及细菌多样性的影响[J].食用菌学报,2022,29(1):27-35.[5]刘炼,赵宁,林茂,等.不同基质及配比对大球盖菇生长和营养成分的影响[J].贵州农业科学,2022,50(3):61-67.[6]SELVAKUMAR P,RAJASEKAR S,BABU A G,et al.Improving biological efficiency of pleurotus strain through protoplast fusion between P.ostreatus var.florida and P.djamor var.roseus[J].Food Science and Biotechnology,2015,24(5):1741-1748.[7]宋莹,刘俊杰,张敏,等.采用原生质体单核体杂交技术创新香菇种质资源[J].北方园艺,2017(19):165-169.[8]刘昆昂,刘萌,张根伟,等.金针菇遗传育种研究进展[J].江苏农业科学,2019,47(14):18-22.[9]李光环,索肖园,史灵燕,等.鲍鱼菇原生质体的制备与再生研究[J].江苏农业科学,2019,47(4):111-113.[10]XIN Y,YANG M,YIN H,et al.Improvement of ethanol tolerance by inactive protoplast fusion in Saccharomyces cerevisiae[J].Biomed Research International,2020,10(2):1-10.[11]冯婷婷,宋天磊,春霞,等.基于响应面法的蟹味菇原生质体制备与再生条件优化[J].北方农业学报,2021,49(1):104-110.[12]李元文.姬菇原生质体技术的研究[D].长沙:湖南农业大学,2006.[13]刘文芳,郭成金.白灵菇原生质体制备与再生研究[J].天津师范大学学报(自然科学版),2012,32(1):88-92.[14]刘士旺,梁宗琦,刘爱英.羊肚菌原生质体制备及其再生[J].西南农业学报,1997,16(4):76-81.[15]江力,刘国庆,窦伟.茶树菇原生质体制备及融合研究[J].食品科学,2008,28(9):348-351.[16]许梅,胡顺珍,贾乐.大球盖菇原生质体制备与再生条件研究[J].生物技术,2006,16(6):57-60.[17]朱静娴.大球盖菇耐高温菌株的选育研究[D].泰安:山东农业大学,2018.[18]张亚从.大球盖菇细胞工程育种及半野生栽培研究[D].保定:河北大学,2011.[19]王谦,刘敏,张亚从.大球盖菇原生质体制备及紫外诱变[J].河北大学学报(自然科学版),2011,31(4):413-417.

Memo

Memo:
-
Last Update: 2023-07-12